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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
  • pan locus tag?: SAUPAN000034000
  • symbol: SAUSA300_0022
  • pan gene symbol?: walH
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
  • symbol: SAUSA300_0022
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 27536..28819
  • length: 1284
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAGTGTCGTATTGACATATATGGTATGGAACTTTTCTCCTGATATTGCAAATGTCGAC
    AATACAGATAGTAAGAAGAGTGAAACGAAACCTTTAACGACACCTATGACAGCCAAAATG
    GATACAACTATTACGCCATTTCAGATTATTCATTCGAAAAATGATCATCCAGAAGGAACG
    ATTGCGACGGTATCTAATGTGAATAAACTGACGAAACCTTTGAAAAATAAAGAAGTGAAG
    TCCGTGGAACATGTTCGTCGTGATCATAACTTGATGATTCCTGATTTGAACAGTGATTTT
    ATATTATTCGATTTTACGTATGATTTACCGTTATCAACATATCTTGGTCAAGTACTGAAC
    ATGAATGCGAAAGTACCAAATCATTTCAATTTCAATCGTTTGGTCATAGATCATGATGCT
    GATGATAATATCGTGCTTTATGCTATAAGCAAAGATCGCCACGATTACGTAAAATTAACA
    ACTACAACGAAAAATGATCATTTTTTAGATGCATTAGCAGCAGTGAAAAAAGATATGCAA
    CCATACACAGATATCATCACAAACAAAGATACAATTGATCGTACGACGCATGTTTTTGCA
    CCAAGTAAACCTGAAAAGTTAAAAACATATCGCATGGTATTTAACACGATTAGTGTTGAG
    AAAATGAATGCTATACTATTTGACGATTCAACCATCGTTCGTAGTTCAAAGAGTGGTGTT
    ACAACCTACAACAATAATACAGGTGTCGCAAACTATAACGATAAAAATGAAAAATATCAT
    TATAAAAACCTGTCCGAAGATGAAGCGAGTTCCAGCAAAATGGAAGAAACGATTCCAGGA
    ACCTTTGATTTTATTAATGGTCATGGTGGTTTCTTAAACGAAGACTTTAGATTGTTTAGT
    ACGAATAATCAGTCAGGCGAGTTAACATATCAACGTTTCCTTAATGGTTATCCAACGTTT
    AATAAAGAAGGTTCTAATCAAATTCAAGTCACTTGGGGTGAAAAAGGCGTCTTTGACTAT
    CGTCGTTCGTTATTACGCACCGACGTTGTTTTAAATAGTGAGGATAATAAATCGTTGCCG
    AAATTAGAGTCTGTACGTTCAAGCTTAGCGAACAATAGTGATATTAATTTTGAAAAAGTA
    ACAAACATCGCTATCGGTTACGAAATGCAGGATAATTCAGATCATAATCACATTGAAGTG
    CAGATTAACAGTGAACTCGTACCGCGTTGGTATGTAGAATATGATGGCGAATGGTATGTT
    TATAACGATGGGAGGCTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1284

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_0022 [new locus tag: SAUSA300_RS00115 ]
  • symbol: SAUSA300_0022
  • description: hypothetical protein
  • length: 427
  • theoretical pI: 5.44435
  • theoretical MW: 49007.3
  • GRAVY: -0.641452

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsTetrapyrrolesCPO analysis FIG011501: YycH protein 
  • PFAM:
    YycI_YycH (CL0285) YycH; YycH protein (PF07435; HMM-score: 427.4)
    no clan definedGly_acyl_tr_N; Aralkyl acyl-CoA:amino acid N-acyltransferase (PF06021; HMM-score: 11)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.244
    • Ymax_pos: 20
    • Cmax: 0.184
    • Cmax_pos: 20
    • Smax: 0.57
    • Smax_pos: 17
    • Smean: 0.294
    • D: 0.264
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSVVLTYMVWNFSPDIANVDNTDSKKSETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHVRRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDNIVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAPSKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKYHYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGYPTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDINFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Olivier Poupel, Mati Moyat, Julie Groizeleau, Luísa C S Antunes, Simonetta Gribaldo, Tarek Msadek, Sarah Dubrac
Transcriptional Analysis and Subcellular Protein Localization Reveal Specific Features of the Essential WalKR System in Staphylococcus aureus.
PLoS ONE: 2016, 11(3);e0151449
[PubMed:26999783] [WorldCat.org] [DOI] (I e)