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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0099 [new locus tag: SAUSA300_RS00515 ]
  • pan locus tag?: SAUPAN000858000
  • symbol: plc
  • pan gene symbol?: plc
  • synonym:
  • product: 1-phosphatidylinositol phosphodiesterase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_0099 [new locus tag: SAUSA300_RS00515 ]
  • symbol: plc
  • product: 1-phosphatidylinositol phosphodiesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 108993..109979
  • length: 987
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAGTGTATTAAGACTTTGTTTTTAAGTATCATTTTAGTAGTGATGAGTGGTTGG
    TATCATTCAGCACATGCGTCAGATTCGTTGAGTAAAAGTCCAGAAAATTGGATGAGTAAA
    CTTGATGATGGAAAACATTTAACTGAGATTAATATACCGGGTTCACATGATAGTGGCTCA
    TTCACTTTAAAGGATCCAGTAAAATCAGTTTGGGCAAAGACTCAAGATAAAGATTACCTT
    ACCCAAATGAAGTCGGGAGTCAGGTTTTTTGATATTAGAGGTAGAGCAAGTGCTGATAAT
    ATGATTTCAGTTCATCACGGCATGGTTTATTTGCATCATGAATTAGGAAAATTTCTCGAT
    GATGCTAAATATTACTTGAGTGCTTATCCAAACGAAACAATTGTGATGTCTATGAAAAAG
    GACTACGATAGCGATTCTAAAGTTACGAAGACATTTGAAGAAATTTTTAGAGAATATTAT
    TATAATAACCCGCAATATCAGAATCTTTTTTACACAGGAAGTAATGCGAATCCTACTTTA
    AAAGAAACGAAAGGTAAAATTGTCCTATTCAATAGAATGGGGGGTACGTACATAAAAAGT
    GGTTATGGTGCTGACACGTCAGGTATTCAATGGGCAGACAATGCGACATTTGAAACGAAA
    ATTAATAATGGTAGCTTAAATTTAAAAGTACAAGATGAGTATAAAGATTACTATGATAAA
    AAAGTTGAAGCTGTTAAAAATTTATTGGCTAAAGCTAAAACGGATAGTAACAAAGACAAT
    GTATATGTGAATTTCTTGAGTGTAGCGTCTGGAGGCAGCGCATTTAATAGTACTTATAAC
    TATGCATCACATATAAATCCTGAAATTGCAAAAACGATTAAAGCAAATGGGAAAGCTAGA
    ACGGGTTGGCTGATTGTTGACTATGCAGGATATACGTGGCCTGGATATGATGATATCGTA
    AGTGAAATTATAGATAGTAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

Protein[edit source | edit]

Protein Data Bank: 3V16
Protein Data Bank: 3V18
Protein Data Bank: 3V1H
Protein Data Bank: 4F2B
Protein Data Bank: 4F2T
Protein Data Bank: 4F2U
Protein Data Bank: 4I8Y
Protein Data Bank: 4I90
Protein Data Bank: 4I9J
Protein Data Bank: 4I9M
Protein Data Bank: 4I9T
Protein Data Bank: 4RV3
Protein Data Bank: 4S3G

General[edit source | edit]

  • locus tag: SAUSA300_0099 [new locus tag: SAUSA300_RS00515 ]
  • symbol: Plc
  • description: 1-phosphatidylinositol phosphodiesterase
  • length: 328
  • theoretical pI: 8.62785
  • theoretical MW: 37086.4
  • GRAVY: -0.555488

Function[edit source | edit]

  • reaction:
    EC 4.6.1.13?  ExPASy
    Phosphatidylinositol diacylglycerol-lyase1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
  • TIGRFAM:
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsPathogenicity islandsListeria Pathogenicity Island LIPI-1 extended Phosphatidylinositol-specific phospholipase C (EC 4.6.1.13) 
  • PFAM:
    PLC (CL0384) PI-PLC-X; Phosphatidylinositol-specific phospholipase C, X domain (PF00388; HMM-score: 65.7)
    Peptidase_CD (CL0093) Raptor_N; Raptor N-terminal CASPase like domain (PF14538; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.623
    • Ymax_pos: 27
    • Cmax: 0.494
    • Cmax_pos: 27
    • Smax: 0.918
    • Smax_pos: 18
    • Smean: 0.797
    • D: 0.691
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDGKHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFNSTYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]