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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
  • pan locus tag?: SAUPAN001187000
  • symbol: SAUSA300_0286
  • pan gene symbol?: esaE
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
  • symbol: SAUSA300_0286
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 341159..341833
  • length: 675
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGATGTTAAGCGAATAGATTATTTTTCTTACGAAGAATTAACAATTTTAGGTGGT
    AGTAAATTGCCTCTCGTAAATTTTGAATTGTTTGATCCATCAAATTTTGAAGAAGCTAAA
    GCTGCTTTAATTGAAAAGGAATTAGTAACAGAGAATGACAAGTTAACTGATGCAGGTTTT
    AAAGTGGCTACATTAGTCAGAGAGTATATTAGCGCCATTGTAAATATTCGAATTAATGAT
    ATGTATTTTGCACCATTTAGCTATGAAAAAGATGAATATATTTTGTTAAGCCGGTTTAAA
    AATAATGGGTTTCAAATACGAATTATCAATAAAGACATTGCATGGTGGTCGATTGTACAA
    TCATATCCTTTATTGATGAGACAAGAAAAGTCCAATGATTGGGACTTTAAACAAATTGAC
    GATGAAACATTGGAGAACTTAAATAATGAAAGTATCGATACGATTGGGCGTGTTTTAGAA
    ATTGAAATATACAATCATCAAGGTGACCCTCAACAAAGTTTATATAACATTTATGAACAA
    AATGATTTGTTATTCATTCGATACCCATTAAAAGATAAAGTGCTGAATGTTCATATTGGT
    GTCATTAATACATTTATACGAGAATTATTTGGATTCGATACTGATGAAAATCATATTAAT
    AAAGCAGAGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
  • symbol: SAUSA300_0286
  • description: hypothetical protein
  • length: 224
  • theoretical pI: 4.33817
  • theoretical MW: 26411.6
  • GRAVY: -0.394643

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • SAV0291 homolog within ESAT-6 gene cluster
    Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  SAV0291 homolog within ESAT-6 gene cluster
  • PFAM:
    no clan defined DUF5081; Domain of unknown function (DUF5081) (PF16887; HMM-score: 285.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014497
    • TAT(Tat/SPI): 0.000187
    • LIPO(Sec/SPII): 0.000337
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALIEKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNNGFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLEIEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENHINKAEE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Mark Anderson, Yi-Hsing Chen, Emily K Butler, Dominique M Missiakas
EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.
J Bacteriol: 2011, 193(7);1583-9
[PubMed:21278286] [WorldCat.org] [DOI] (I p)
Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)