NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0650 [new locus tag: SAUSA300_RS03485 ]
• pan locus tag^?: SAUPAN002544000
• symbol: SAUSA300_0650
• pan gene symbol^?: pitA
• synonym:
• product: phosphate transporter family protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0650 [new locus tag: SAUSA300_RS03485 ]
• symbol: SAUSA300_0650
• product: phosphate transporter family protein
• replicon: chromosome
• strand: +
• coordinates: 724867..725874
• length: 1008
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914090 NCBI
• RefSeq: YP_493353 NCBI
• MicrobesOnline: 1292165 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCATATATAATCATCGTCACTATAGCTGTAGTTATTTTCTCGCTGATATTTGACTTT
  ATCAATGGATTCCATGATACAGCCAATGCAGTAGCTACTGCTGTATCTACTAGAGCGTTA
  ACGCCTAAAACGGCAATTTTAATGGCAGCAGTGATGAACTTTATAGGTGCTTTAACATTT
  ACGGGCGTTGCAGGCACCATTACTAAAGACATTGTCGATCCATTTAAATTGGAAAATGGA
  TTAGTTGTTGTGTTAGCTGCAATACTTGCGGCTATTATTTGGAATTTAGCTACTTGGTTT
  TACGGAATTCCAAGTTCGTCTTCACATGCACTTATAGGTTCAATTGCGGGTGCAGCAATC
  GCATCTGAAGGCTCATTTGGAGTGTTACATTACCAAGGTTTCACAAAAATTATTATTGTA
  TTAATCGTTTCACCGATTATCGCATTTTGTGTTGGTTTCTTGATGTATTCAATTTTTAAA
  GTTATCTTTAAAAATGCAAATTTAACAAGAGCGAATCGTAACTTTAGATTTTTCCAAATT
  TTCACAGCAGCGTTACAATCATTCTCTCACGGTACGAATGATGCGCAAAAATCAATGGGT
  ATTATTACGTTGGCATTGATTGTCGCTAATGTACAGAATGATGGCAGTGTTGAACCACAG
  TTATGGGTAAAATTTGCCTGTGCGACAGCAATGGGGCTTGGTACTGCAATTGGTGGCTGG
  AAAATTATCAAAACTGTAGGTGGTAATATTATGAAAATACGTCCAGCAAATGGTGCTGCG
  GCCGATTTATCATCTGCATTAACAATTTTTGTTGCATCATCGCTACATTTCCCATTATCA
  ACAACTCACGTTGTGTCATCATCAATCTTAGGTGTTGGTGCTTCTAACCGAGCTAAAGGT
  GTAAAATGGAGCACTGCGCAACGAATGATCATTACATGGGTGATTACATTACCTATTTCA
  GCATTGTTAGCAGGTTTACTATTCTATATACTTAACTTATTTTTCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0650 [new locus tag: SAUSA300_RS03485 ]
• symbol: SAUSA300_0650
• description: phosphate transporter family protein
• length: 335
• theoretical pI: 10.3947
• theoretical MW: 35587.8
• GRAVY: 0.891045

⊟Function

• reaction:
• TIGRFAM:
• TheSEED:  
  Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Probable low-affinity inorganic phosphate transporter 
• PFAM:
  no clan definedPHO4; Phosphate transporter family (PF01384; HMM-score: 305.4)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.374
  • Ymax_pos: 31
  • Cmax: 0.317
  • Cmax_pos: 31
  • Smax: 0.573
  • Smax_pos: 18
  • Smean: 0.377
  • D: 0.375
• predicted transmembrane helices (TMHMM): 7

⊟Accession numbers

• GI: 87161402 NCBI
• RefSeq: YP_493353 NCBI
• UniProt: A0A0H2XH71 UniProt

⊟Protein sequence

• MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKTAILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYGIPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFKVIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVEPQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLHFPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLFF

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0649 > SAUSA300_0650

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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