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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0883 [new locus tag: SAUSA300_RS04760 ]
  • pan locus tag?: SAUPAN003124000
  • symbol: SAUSA300_0883
  • pan gene symbol?:
  • synonym:
  • product: putative surface protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0883 [new locus tag: SAUSA300_RS04760 ]
  • symbol: SAUSA300_0883
  • product: putative surface protein
  • replicon: chromosome
  • strand: +
  • coordinates: 970111..970545
  • length: 435
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATTAAAATCATTTATAACTGTAACTTTGGCACTGGGCATGATCGCAACGACTGGC
    GCTACTGTGGCAGGTAATGAGGTATCTGCAGCAGAAAAGGACAAACTACCGGCAACTCAA
    AAAGCTAAAGAAATGCAAAATGTTCCATATACAATTGCAGTAGATGGCATTATGGCTTTC
    AATCAATCTTACTTAAATTTACCAAAAGATAGCCAATTATCATATTTAGATTTAGGAAAT
    AAAGTTAAAGCTTTGTTATATGATGAACGCGGTGTAACACCTGAGAAGATTCGAAATGCA
    AAATCTGCCGTTTACACGATTACTTGGAAAGATGGTAGTAAAAAAGAAGTGGATCTTAAG
    AAAGATAGCTACACAGCAAACTTGTTTGATTCAAATTCAATTAAACAAATTGATATTAAT
    GTAAAAACTAAATAA
    60
    120
    180
    240
    300
    360
    420
    435

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0883 [new locus tag: SAUSA300_RS04760 ]
  • symbol: SAUSA300_0883
  • description: putative surface protein
  • length: 144
  • theoretical pI: 9.84615
  • theoretical MW: 15838.1
  • GRAVY: -0.342361

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Similar to cell surface protein Map-w
  • PFAM:
    Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 121.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: NEVSAAEK
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.973909
    • TAT(Tat/SPI): 0.003194
    • LIPO(Sec/SPII): 0.020514
    • Cleavage Site: CS pos: 30-31. VSA-AE. Pr: 0.7117
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKEMQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKSAVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]