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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1239 [new locus tag: SAUSA300_RS06725 ]
  • pan locus tag?: SAUPAN003717000
  • symbol: tkt
  • pan gene symbol?: tkt
  • synonym:
  • product: transketolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1239 [new locus tag: SAUSA300_RS06725 ]
  • symbol: tkt
  • product: transketolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1356635..1358512
  • length: 1878
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGCTTACACTTTGTGGACACGTCATCTGAATTTTAATCCACAATCTAAAGATTACTTC
    AATAGAGACCGTTTCGTATTATCTGCAGGGCATGGTTCAGCATTATTGTATAGCTTGTTA
    CATGTTTCTGGTAGTTTAGAATTAGAAGAATTAAAGCAATTTAGACAATGGGGTTCTAAA
    ACACCAGGTCATCCTGAATACAGACATACAGATGGTGTAGAAGTTACTACCGGACCACTT
    GGACAAGGTTTTGCTATGTCAGTAGGATTAGCTTTAGCAGAAGATCACCTAGCAGGGAAA
    TTTAATAAAGAAGGATATAATGTTGTAGATCATTACACATATGTATTAGCTTCTGACGGT
    GATTTAATGGAAGGTATATCGCATGAAGCAGCTTCATTTGCTGGACATAATAAATTAAGT
    AAATTAGTTGTTTTATACGATTCAAATGATATTTCATTAGATGGCGAATTAAACAAAGCT
    TTTTCTGAAAACACAAAAGCTCGTTTTGAAGCATATGGTTGGAATTACTTACTAGTTAAA
    GATGGTAATGATTTAGAAGAAATTGATAAAGCGATTACTACAGCTAAATCTCAAGAAGGA
    CCAACGATTATTGAAGTTAAAACAACAATCGGATTTGGTTCACCGAATAAAGCAGGAACT
    AATGGTGTTCATGGGGCACCTTTAGGTGAAGTTGAAAGAAAATTAACATTCGAAAATTAC
    GGTTTAGATCCTGAAAAACGTTTTAATGTTTCAGAAGAGGTATACGAAATTTTCCAAAAT
    ACTATGTTAAAACGTGCTAATGAAGATGAATCTCAATGGAATTCATTATTAGAAAAATAT
    GCAGAAACATATCCTGAATTAGCAGAAGAATTTAAATTAGCGATTAGTGGTAAATTGCCT
    AAAAATTATAAGGATGAATTACCACGTTTTGAACTGGGTCATAATGGTGCATCTCGTGCT
    GATTCTGGTACTGTTATTCAAGCAATCAGTAAAACTGTCCCTTCATTCTTTGGTGGATCA
    GCAGACCTTGCTGGTTCAAACAAATCCAATGTAAATGATGCAACTGATTATAGTTCTGAA
    ACACCTGAAGGTAAAAATGTGTGGTTTGGTGTACGTGAATTTGCTATGGGTGCTGCTGTA
    AATGGTATGGCTGCACATGGAGGTTTACATCCATATGGTGCAACATTCTTCGTATTTAGT
    GATTATTTAAAACCAGCGTTACGTTTATCATCAATTATGGGATTAAATGCAACGTTCATC
    TTCACACATGATTCAATTGCAGTAGGTGAAGATGGTCCTACTCATGAACCAATTGAGCAA
    TTAGCTGGATTAAGAGCCATTCCAAATATGAATGTTATCCGTCCTGCTGATGGTAATGAA
    ACAAGAGTAGCATGGGAAGTTGCCTTAGAATCTGAATCTACACCTACTTCATTAGTATTG
    ACACGTCAAAACTTACCGGTATTAGATGTACCAGAAGATGTAGTTGAAGAAGGCGTTCGA
    AAAGGTGCCTATACAGTTTATGGCTCTGAAGAGACACCAGAATTCCTATTATTAGCTTCA
    GGTTCAGAAGTTAGTCTTGCAGTTGAAGCTGCTAAAGATCTTGAAAAACAAGGTAAATCA
    GTACGTGTTGTTTCAATGCCTAACTGGAATGCATTTGAACAACAATCTGAAGAATATAAA
    GAATCAGTTATTCCATCAAGCGTAACAAAACGTGTTGCGATTGAAATGGCTTCACCGCTT
    GGATGGCATAAATATGTAGGTACTGCAGGTAAAGTTATTGCTATTGACGGCTTTGGCGCA
    AGTGCACCTGGCGATTTAGTAGTTGAAAAATATGGATTTACAAAAGAAAATATCTTAAAC
    CAAGTTATGAGCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1878

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1239 [new locus tag: SAUSA300_RS06725 ]
  • symbol: Tkt
  • description: transketolase
  • length: 625
  • theoretical pI: 4.71462
  • theoretical MW: 68358.9
  • GRAVY: -0.3384

Function[edit source | edit]

  • reaction:
    EC 2.2.1.1?  ExPASy
    TransketolaseSedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismPentose phosphate pathwaytransketolase (TIGR00232; EC 2.2.1.1; HMM-score: 860.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 71.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 71.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 71.3)
    pyruvate dehydrogenase (acetyl-transferring), homodimeric type (TIGR00759; EC 1.2.4.1; HMM-score: 34.3)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 21)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 17.7)
    alpha-ketoglutarate dehydrogenase (TIGR03186; HMM-score: 15.7)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPentose phosphate pathway Transketolase (EC 2.2.1.1) 
  • PFAM:
    THDP-binding (CL0254) Transketolase_N; Transketolase, thiamine diphosphate binding domain (PF00456; HMM-score: 411.4)
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 135.3)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 38.6)
    THDP-binding (CL0254) DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 25.1)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 17.9)
    E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 14.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, thiamine diphosphate
  • effectors:
  • protein partners:
    SAUSA300_1640(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_2201(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SAUSA300_0527(rpoB)DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_1305(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0658LysR family transcriptional regulator  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.135
    • Ymax_pos: 37
    • Cmax: 0.112
    • Cmax_pos: 48
    • Smax: 0.222
    • Smax_pos: 32
    • Smean: 0.145
    • D: 0.139
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAYTLWTRHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHPEYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLMEGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKDGNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFENYGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISGKLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDATDYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIMGLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALESESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVEAAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVGTAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]