From AureoWiki
Jump to: navigation, search
04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
  • pan locus tag?:
  • symbol: SAUSA300_1410
  • pan gene symbol?:
  • synonym:
  • product: virulence-associated protein E

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
  • symbol: SAUSA300_1410
  • product: virulence-associated protein E
  • replicon: chromosome
  • strand: -
  • coordinates: 1575087..1577534
  • length: 2448
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTTAGACAAAGTCACTCAAATAGAAACAATTAAATATGATCGTGATGTCTCATATTCT
    TATGCTGCTAGTCGTTTATCCACATATTGGACTAATCACAATATGGCTTGGTCTGACTTT
    ATGCAGAAGCTAGCACAAACAGTTAGAACTAAAGAAGATTTAACTGAGTACAATAAAATG
    TCTAAGTCTGAACAAGCAGATATAAAAGATGTTGGCGGATTTGTCGGTGGATATTTAAAA
    GAAGGCAAACGACGTGCTGGTCAAGTCATGAATCGTTCAATGCTAACACTTGATATCGAT
    TATGCTGCTCAAGATATGACTGACATATTATCTATGTTTTATGATTTTGCATATTGTTTA
    TATTCAACACATAAGCATAGAGAGATAAGTCCAAGACTGCGTTTAGTGATTCCTTTAAAA
    CGAAATGTAAATGCAGATGAGTATGAAGCTATTGGGCGTAAAGTCGCAGATATCGTTGGC
    ATGGATTACTTCGATGATACAACTTATCAACCACATAGGTTAATGTATTGGCCTTCAACT
    AGCAATGATGCAGAATTTTTCTTTACCTATGAAGATTTACCTTTGTTAGATCCAGATAAA
    ATATTAAATGAATATGTTGATTGGACTGACACATTAGAATGGCCAACGTCTTCAAAGGAA
    GAGAGTAAGACTAAAAGATTAGCAGATAAGCAAGGTGACCCAGAAGAAAAGCCGGGAATT
    GTTGGCGCATTTTGTAGAGCCTATACGATAGAAGAAGCTATATCAACTTTTATTCCTGAC
    TTATACGAAAAACATTCTACTAACCGTTATACCTATCATGAAGGTTCAACTGCAGGTGGA
    TTGGTGTTATACGAAAATAACAAGTTTGCCTATTCTCATCATAATACGGATCCCGTTAGC
    GGTATGCTTGTGAACAGTTTTGATTTAGTACGCATACACTTATATGGTGCTCAAGATGAA
    GACGCTAAAACAGATACTCCGGTTAATCGACTACCTAGTTATAAAGCAATGCAGCAAAGA
    GCGCAAAATGATGAAGTTGTTAAAAAGCAATTAATTAACGACAAAATGTCTGATGCAATG
    CAGGATTTCGATGAAATAGTAAATAGCGATGATGCATGGTCTGAGACGTTAGAAATTACT
    TCGAAAGGTACTTTCAAAGCTAGTATCCCAAATATAGAAATTATATTGCGTAATGATCCA
    AATTTAAAAGGAAAAATAGCATTTAATGAATTTACAAAACAAATTGAATGCTTAGGGAAA
    GTGCCATGGAATACTAATTTTAAGACACGTCAATGGCAAGACGGTGATGATAGCAGTTTA
    AGAAGTTATATCGAAAAGATTTATGACATACACCATTCAGGTAAAACAAAAGATGCCATT
    ATAAGCGTAGCAATGCAAAATGCTTATCATCCAGTAAGGGATTATCTAAATAAAATATCG
    TGGGATGGACATAAACGTCTTGAAAAGTTATTTATCAAATACTTAGGTGTTGAAGATACT
    GAAGTGAATAGAACAACTACCAAAAAAGCATTGACTGCTGGAATTGCTCGAGTAATGGAG
    CCTGGATGTAAATTTGACTATATGCTTACACTTTATGGTCCTCAAGGTGTAGGTAAATCT
    GCTTTGCTAAAAAAATTAGGTGGTGCATGGTTTTCTGACAGTTTAGTTTCTGTTACAGGT
    AAAGAAGCCTATGAGGCCTTACAAGGCGTTTGGCTAATGGAAATGGCAGAACTTGCAGCT
    ACAAGAAAAGCTGAAGTTGAAGCTATTAAGCATTTCATATCTAAACAAGTTGACCGGTTT
    CGTGTTGCTTATGGACATTATATTGAAGATTTTCCAAGGCAATGTATTTTCATTGGTACA
    ACTAATAAAGTTGATTTCTTAAGAGATGAAACTGGTGGAAGACGTTTTTGGCCAATGACT
    GTAAATCCAGAGAGAGTTGAAGTGAACTGGTCTAAACTAACCAAAGAAGAGATCGACCAA
    ATTTGGGCAGAAGCTAAATATTATTATGAACAAGGAGAAGAGTTATTCCTCAACCCTGAA
    CTAGAAGAAGAAATGCGTTCAATACAAAGCAAACATACTGAGGAATCTCCATATACAGGC
    ATTATTGATGAATATCTTAACACACCAATTCCTAGCAATTGGGATGACTTAACTATCTTT
    GAACGAAGACGATTTTATCAAGGTGATGTTGATATGTTACCAACAGGAAATGTAGATTAC
    GTTGAAAGAAATAAGGTCTGTGCGCTTGAAGTGTTTGTTGAATGTTTTGGTAAAGATAAG
    GGAGATAGTAGAGGATCTATGGAAATTAGAAAGATTTCAAACATCTTAAGACAATTAGAC
    AATTGGTCTGTATATGATGGTAATAAAAGTGGGAAAATTCGATTTGGAAAAGATTATGGT
    GTACAGATAGCTTATGTAAGAGATGAAAGTTTAGAAGATTTAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2448

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1410 [new locus tag: SAUSA300_RS07695 ]
  • symbol: SAUSA300_1410
  • description: virulence-associated protein E
  • length: 815
  • theoretical pI: 5.02446
  • theoretical MW: 94187.4
  • GRAVY: -0.61362

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    DNA primase, phage associated 
  • PFAM:
    P-loop_NTPase (CL0023) VirE; Virulence-associated protein E (PF05272; HMM-score: 289.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.3)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.4)
    AAA_22; AAA domain (PF13401; HMM-score: 13)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.8)
    NACHT; NACHT domain (PF05729; HMM-score: 12.1)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 11.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 11.8)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 11.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.142
    • Ymax_pos: 39
    • Cmax: 0.209
    • Cmax_pos: 39
    • Smax: 0.157
    • Smax_pos: 35
    • Smean: 0.092
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKLAQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYAAQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVGMDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSSKEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELEEEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDYVERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKDYGVQIAYVRDESLEDLI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]