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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1456 [new locus tag: SAUSA300_RS07950 ]
  • pan locus tag?: SAUPAN004042000
  • symbol: SAUSA300_1456
  • pan gene symbol?: malA
  • synonym:
  • product: alpha-D-1,4-glucosidase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1456 [new locus tag: SAUSA300_RS07950 ]
  • symbol: SAUSA300_1456
  • product: alpha-D-1,4-glucosidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1609651..1611300
  • length: 1650
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAAGCAATGGTGGAAAGAAGCAGTAGCATATCAAGTATATCCAAGAAGTTTTAAT
    GATAGTAATCACGATGGTATTGGGGATTTACCTGGAATGATTGATAAATTGGACTACTTA
    AAAGATTTCGGTATCGATGTCATTTGGCTCAGTCCAATGTTTAAATCACCTAATGATGAC
    AATGGTTATGATATTAGTGACTACCAAGAGATTATGGATGAATTTGGAACGATGGAAGAC
    TTTGATCGTTTATTAAAAGGTGTTCATGATAGAGGCATGAAGCTTATTTTAGATTTAGTT
    GTAAATCATACATCTGATGAACATCCTTGGTTTATAGAATCCAAATCTAGTAAAGACAAT
    CCCAAACGTGATTGGTACATTTGGCAAGATCCAAAGCCAGATGGCTCTGAACCTAACAAC
    TGGGAAAGTATATTTAATGGATCTACATGGGAATATGATGCTAATACTGAGCAATATTAT
    TTCCATTTATTCAGTAAAAAACAACCTGATTTGAATTGGGGTAATCCGGAAGTTAGAGAT
    GCTGTATTTGAAATGATGAACTGGTGGTTTGATAAAGGCATTGATGGATTTAGAGTAGAT
    GCAATTACGCATATTAAGAAGACGTTTGAAGCGGGTGACTTACCTGTACCTGAGGGTAAA
    ACATATGCCCCAGCATTTGATGTAGATATGAATCAGCCAGGTATACAAACTTGGTTACAA
    GAGATGAAAGATCGCTCATTAAGTAAGTATGACATTATGACTGTTGGTGAAGCGAATGGT
    GTAAGCCCTGATGATGCTGATGACTGGGTCGGGGAAGAAAATGGTAAATTTAATATGATA
    TTCCAATTTGAACATTTGGGACTGTGGAATAGTGGTGATTCTCACTTTGATGTAAATTCG
    TATAAATCTGTATTAAATAGATGGCAAAAACAACTTGAAAATAAAGGTTGGAATGCGTTG
    TTTATTGAAAATCACGACCAACCACGACGTGTATCGACGTGGGGTGACGATGACAAGTAT
    TGGTATGAATCAGCAACAAGTCATGCAGCTGTTTATTTCTTGCAACAAGGTACGCCATTC
    ATTTATCAAGGTCAAGAAATTGGTATGACGAATTATCCATTTGAAAGTATTGAAACGTTT
    AACGATGTTGCTGTTAAAAATGACTATCAAATAGTGAAAGCTCAAGGTGGAGATGTAGAC
    GCTTTACTTGCGAAATATAAAGATGAGAACCGAGATAATTCTCGCACACCAATGCAATGG
    GATGATACGTTAAATGGAGGATTTACAAATGGTGAACCGTGGTTCCCAGTGAATCCGAAT
    TATAAAACTATCAATGTTGCACAACAATTAGAAGATGAGCATTCAGTATTACAATTTTAT
    AAAGATTTAATTCAATTAAGAAAGTCTAATGATGTATACGTATATGGTCAATTTGATTTA
    GTAGATGCTGAAAATTCACAAGTTTTTGCGTACACGAGAACATTAAATGAAAAGCAAGTT
    CTTATTGTAGGTAATCTTACTAACCACGAAGCAGAATTAACTGTACCATTTGATTTAAGC
    CATGGAGAAGTAAAGCTATTTAATTATGATGCCAAAGTTAATTTAAAACAGTTACGTCCA
    TATGAAGCATGTGTTATCGAACTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1650

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1456 [new locus tag: SAUSA300_RS07950 ]
  • symbol: SAUSA300_1456
  • description: alpha-D-1,4-glucosidase
  • length: 549
  • theoretical pI: 4.30515
  • theoretical MW: 63905.2
  • GRAVY: -0.736066

Function[edit | edit source]

  • reaction:
    EC 3.2.1.1?  ExPASy
    Alpha-amylase Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
    EC 3.2.1.20?  ExPASy
    Alpha-glucosidase Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 597.7)
    and 9 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 346.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 66.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 62.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 29.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 25.7)
    pullulanase, extracellular (TIGR02102; HMM-score: 24.4)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 22.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme (TIGR01531; HMM-score: 12.8)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 12.4)
  • TheSEED  :
    • Alpha-glucosidase (EC 3.2.1.20)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Alpha-glucosidase (EC 3.2.1.20)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 380.6)
    and 4 more
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 34.7)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 17.8)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.5)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007678
    • TAT(Tat/SPI): 0.000178
    • LIPO(Sec/SPII): 0.000843
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFGIDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVNHTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYYFHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPEGKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGKFNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTWGDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIVKAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQLEDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTNHEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: CcpA regulon, MalR (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    MalR(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]