NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1899 [new locus tag: SAUSA300_RS10395 ]
• pan locus tag^?: SAUPAN004953000
• symbol: SAUSA300_1899
• pan gene symbol^?: pncA
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1899 [new locus tag: SAUSA300_RS10395 ]
• symbol: SAUSA300_1899
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2065707..2066267
• length: 561
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914700 NCBI
• RefSeq: YP_494551 NCBI
• MicrobesOnline: 1293414 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  541
  
  ATGACACATCGCGCACTATTAGTTGTTGACTATTCATATGACTTTATCGCAGACGACGGC
  TTACTAACATGCGGTAAACCTGGACAAAATATTGAAGATTTTATTGTTTCTCGTATCAAT
  GACTTTAATTATTATCAAGACCATATATTCTTTTTAATGGATTTACATTATTTACATGAC
  ATTCATCATCCTGAAAGTAAATTATTCCCACCACACAATATCGTAGATACAAGTGGTAGA
  GAATTATACGGTAAAGTAGGTAAATTATACGAAACAATTAAAGCGCAACCTAATGTACAT
  TTCATTGATAAAACGCGCTATGATTCGTTCTTTGGTACCCCGCTTGATAGTTTATTGAGA
  GAAAGAAGTATTAATCAAGTCGAAATCGTTGGTGTATGTACCGATATTTGCGTGTTACAT
  ACAGCAATTTCTGCATACAACTTAGGTTATAAAATTTCAGTACCTGCTGAGGGAGTGGCT
  TCATTTAATCAAAAAGGGCATGAATGGGCACTTGCACATTTCAAAAACTCATTAGGTGCA
  GAGGTAGAACAACACGTTTAA
  60
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  561
  

⊟Protein

⊟General

• locus tag: SAUSA300_1899 [new locus tag: SAUSA300_RS10395 ]
• symbol: SAUSA300_1899
• description: hypothetical protein
• length: 186
• theoretical pI: 6.05943
• theoretical MW: 21198.8
• GRAVY: -0.187097

⊟Function

• TIGRFAM:
  pyrimidine utilization protein B (TIGR03614; HMM-score: 41.5)
• TheSEED:  
  Cofactors, Vitamins, Prosthetic Groups, PigmentsNAD and NADPNAD and NADP cofactor biosynthesis global Nicotinamidase (EC 3.5.1.19) 
  and 1 more
  Stress ResponseOxidative stressRedox-dependent regulation of nucleus processes Nicotinamidase (EC 3.5.1.19) 
• PFAM:
  no clan definedIsochorismatase; Isochorismatase family (PF00857; HMM-score: 107.1)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.106
  • Ymax_pos: 31
  • Cmax: 0.115
  • Cmax_pos: 31
  • Smax: 0.117
  • Smax_pos: 21
  • Smean: 0.076
  • D: 0.094
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162153 NCBI
• RefSeq: YP_494551 NCBI
• UniProt: A0A0H2XK13 UniProt

⊟Protein sequence

• MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNYYQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFIDKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVASFNQKGHEWALAHFKNSLGAEVEQHV

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1899 > ppaC

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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