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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1969 [new locus tag: SAUSA300_RS10810 ]
  • pan locus tag?: SAUPAN005125000
  • symbol: SAUSA300_1969
  • pan gene symbol?:
  • synonym:
  • product: phi77 ORF011-like protein, phage transcriptional repressor

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1969 [new locus tag: SAUSA300_RS10810 ]
  • symbol: SAUSA300_1969
  • product: phi77 ORF011-like protein, phage transcriptional repressor
  • replicon: chromosome
  • strand: +
  • coordinates: 2123236..2123949
  • length: 714
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCA
    AATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACTTTAAGCGAT
    TATTTAAATTTACGTTCCAACCCCTCTCACGGCGTTATACAAAGGATAGCTGATGTTTTC
    GAGGTTGGTAAAAGCGACATAGATACTACATACAAAGACGATAACGACATCACTTCCATA
    TACAACAAACTCACACCTCCCCGCCAAGAAAACGTACTTAACTATGCAAATGAACAATTG
    GAAGAACAGAATTCTAAAGGAGATAACGTTGTAGATATTAATTCATATAAACAGGAGAAA
    ACTCCAGTTAACGTCAATGGTTGCGTCTCTGCTGGTGTAGGAGAACGTTTACACGATGAA
    ACGCTATTTACTGAAATGGTTAAAGGACCTATCCCCACACACGATTTAGCGTTAAAAGTA
    AATGGTGATTCTATGGAACCTATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACT
    CACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAG
    AAAGTCTTTGTTGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAT
    CTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    714

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1969 [new locus tag: SAUSA300_RS10810 ]
  • symbol: SAUSA300_1969
  • description: phi77 ORF011-like protein, phage transcriptional repressor
  • length: 237
  • theoretical pI: 5.05491
  • theoretical MW: 27017.4
  • GRAVY: -0.528692

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 41.6)
    Signal transduction Regulatory functions DNA interactions repressor LexA (TIGR00498; EC 3.4.21.88; HMM-score: 41.6)
    and 9 more
    signal peptidase I (TIGR02228; EC 3.4.21.89; HMM-score: 26)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking signal peptidase I (TIGR02227; EC 3.4.21.89; HMM-score: 24.4)
    Cellular processes Cellular processes Detoxification nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 16.7)
    Genetic information processing Protein fate Protein modification and repair nickel-type superoxide dismutase maturation protease (TIGR02754; EC 3.4.21.-; HMM-score: 16.7)
    ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 15.9)
    Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
    Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
    Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 15.3)
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 12.5)
  • TheSEED  :
    • Phage repressor protein cI
  • PFAM:
    Peptidase_SF (CL0299) Peptidase_S24; Peptidase S24-like (PF00717; HMM-score: 60)
    and 14 more
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 42.8)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 29.3)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 24.2)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 21.8)
    HNF-1_N; Hepatocyte nuclear factor 1 (HNF-1), N terminus (PF04814; HMM-score: 20.7)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 16.9)
    HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 14.9)
    PELOTA (CL0101) TSNR_N; Thiostrepton-resistance methylase, N terminus (PF04705; HMM-score: 13.4)
    PDDEXK (CL0236) PDDEXK_10; PD-(D/E)XK nuclease superfamily (PF07788; HMM-score: 13.2)
    HTH (CL0123) CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 13.1)
    YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 13)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 12.2)
    MarR; MarR family (PF01047; HMM-score: 12.1)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004779
    • TAT(Tat/SPI): 0.000511
    • LIPO(Sec/SPII): 0.000535
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNLRSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEEQNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKVNGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDYDDLHFYRNESVRLIGKVIL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]