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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2071 [new locus tag: SAUSA300_RS11405 ]
  • pan locus tag?: SAUPAN005408000
  • symbol: SAUSA300_2071
  • pan gene symbol?: prmC
  • synonym:
  • product: HemK family modification methylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2071 [new locus tag: SAUSA300_RS11405 ]
  • symbol: SAUSA300_2071
  • product: HemK family modification methylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2232834..2233586
  • length: 753
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTAGATGTATTTCAATGGACGCGTACGGACTTTGTAGTCCACATGCATGATGATATG
    CCGAAAGCGATGATTATGAAGTTCGACTTAGCATTACAACGTATGTTATTAGGAGAGCCT
    ATACAGTATATAGTTGGCTTTGCCTCATTTTATGGTAGAACGTTTGATGTAAACTCAAAT
    TGTTTGATACCAAGACCTGAAACTGAAGAAGTAATGTTGCATTTCTTACAACAGTTAGAA
    GATGATGCAACAATCGTAGATATCGGAACGGGTAGTGGTGTACTTGCAATTACTTTGAAA
    TGTGAAAAGCCGGATTTAAATGTTATTGCTACTGATATTTCACTTGAAGCAATGAATATG
    GCTCGTAATAATGCTGAGAAGCATCAATCACAAATACAATTTTTAACAGGGGATGCATTA
    AAGCCCTTAATTAATGAAGGTATCAAGTTGAACGGCTTGATATCTAATCCACCATATATA
    GATGAAAAAGATATGGTTACGATGTCTCCAACGGTTACGAGATTCGAACCACATCAGGCA
    TTGTTTGCAGATAACCATGGATATGCTATTTATGAATCAATCATTGAAGATTTACCTCAC
    GTTATGGAAAAAGGCAGCCCAGTTGTTTTTGAAATTGGTTACAATCAAGGTGAGGCACTT
    AAATCAATAATTTTAAATAAATTTCCTGACAAAAAAATCGACATTATTAAAGATATAAAT
    GGCCACGATCGAATCGTCTCATTTAAATGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2071 [new locus tag: SAUSA300_RS11405 ]
  • symbol: SAUSA300_2071
  • description: HemK family modification methylase
  • length: 250
  • theoretical pI: 4.69126
  • theoretical MW: 28341.5
  • GRAVY: -0.1072

Function[edit | edit source]

  • reaction:
    EC 2.1.1.297?  ExPASy
    Peptide chain release factor N5-glutamine methyltransferase S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 253.6)
    and 21 more
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 201)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 114.9)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 71.9)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 47.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 33.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 30.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 29)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 27.6)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 27)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 23.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 23.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 21)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 18.9)
    Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 15.4)
    Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 14.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 14.1)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 14)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 13.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification N2,N2-dimethylguanosine tRNA methyltransferase (TIGR00308; EC 2.1.1.-; HMM-score: 12.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 11.9)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 11.1)
  • TheSEED  :
    • Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2
    RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2
  • PFAM:
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 58)
    and 19 more
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 34.6)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 34.2)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 33.7)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 32)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 26.2)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 24.1)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 24.1)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 22.2)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 21.2)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 19.9)
    Methyltransf_2; O-methyltransferase (PF00891; HMM-score: 19.7)
    Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 19.3)
    GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 17.7)
    CmcI; Cephalosporin hydroxylase (PF04989; HMM-score: 17.1)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.9)
    TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 16.2)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 14.1)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 12.9)
    CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 11.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001482
    • TAT(Tat/SPI): 0.000211
    • LIPO(Sec/SPII): 0.000177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

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Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]