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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2271 [new locus tag: SAUSA300_RS12555 ]
  • pan locus tag?: SAUPAN005805000
  • symbol: SAUSA300_2271
  • pan gene symbol?: glvR
  • synonym:
  • product: phosphosugar-binding transcriptional regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2271 [new locus tag: SAUSA300_RS12555 ]
  • symbol: SAUSA300_2271
  • product: phosphosugar-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2441889..2442653
  • length: 765
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTTTTTAGATGAACACATTAATCGAAACTTTGATAAACTTAATGATAATGATTTACAT
    ATCGCTCACTTTATCAATACACATATAGATGAATGTAAAAATATGAAAATACAAGATTTA
    GCGCAATTCACACATGCCTCGAATGCAACCATTCACAGATTTACACGCAAATTAGGTTTT
    GACGGTTATAGTGATTTTAAATCGTACCTTAAATTTGAAAGTAATAAGACACACCAATTA
    CCTTCTAACTCTATCGATAGTTTCAAACAAGAAATCGATAGTACATTCAGTTATTTAGAA
    CGCGTTGATTACCAGCTTCTTACTAAAAAAATGCATACAGCTTCAACAATATATTTGTAT
    GGTACAGGGCGTGCACAGTTAAACGTTGCTTCAGAGGCACAGCGTATATTGTTAACGTTA
    CATAATCATGTCATTGTACTTCATGATGAACATGAACTTAAAATGATTTTGAACAAGAGT
    GAACCTAATGATTTATTCTTTATTATTTCATTATCAGGTGAAACACATGAATTATTAGAA
    GTAACAAACTTGCTTCAACTTAGACAAAAATACTTTATTTCTGTTACTACAATGAAAGAT
    AATACCCTTGCTCAAAAAGCAAATTACAACGTCTATGTATCCAGTAACACATTCTACTTA
    ACTGATGGTACAGACTATTCTAGCTTCATCAGTTATCATATATTCTTTGAAACACTTGTA
    AGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2271 [new locus tag: SAUSA300_RS12555 ]
  • symbol: SAUSA300_2271
  • description: phosphosugar-binding transcriptional regulator
  • length: 254
  • theoretical pI: 6.56496
  • theoretical MW: 29784.3
  • GRAVY: -0.423622

Function[edit | edit source]

  • TIGRFAM:
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 47.3)
    and 4 more
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 26.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 19.4)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 19.4)
    bifunctional phosphoglucose/phosphomannose isomerase (TIGR02128; EC 5.3.1.9; HMM-score: 13.2)
  • TheSEED  :
    • Transcriptional regulator, RpiR family
  • PFAM:
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 64.3)
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 60.6)
    and 3 more
    SIS_2; SIS domain (PF13580; HMM-score: 14.4)
    HTH (CL0123) HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 14.3)
    MarR; MarR family (PF01047; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Maltose-6-phosphate
  • gene regulated by GlvR*, TF important in Maltose utilizationRegPrecise
    activation

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004508
    • TAT(Tat/SPI): 0.000748
    • LIPO(Sec/SPII): 0.00093
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFTHASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERVDYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKSEPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTFYLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]