From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • pan locus tag?: SAUPAN005915000
  • symbol: narK
  • pan gene symbol?: narK
  • synonym:
  • product: nitrite extrusion protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • symbol: narK
  • product: nitrite extrusion protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2508369..2509538
  • length: 1170
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTACAAAACAAAAGGTGGCTTTCAACTTACATTACAAACATTAAGTTTAGTGGTTGGG
    TTTATGGCTTGGAGTATAATTGCGCCATTAATGCCCTTTATTAAACAAGATGTCAATGTT
    ACTGAAGGTCAAATATCAATCATTTTAGCGATACCAGTTATTTTGGGATCGGTGCTCCGT
    GTGCCATTTGGTTATTTAACAAACATTGTTGGCGCTAAATGGGTATTCTTTACTAGTTTT
    ATCGTATTGTTATTCCCGATATTTTTCTTAAGCCAAGCACAAACACCGGGTATGTTAATG
    GCTTCAGGATTTTTCCTTGGTGTAGGTGGTGCAATTTTCTCAGTTGGTGTTACATCAGTT
    CCTAAATATTTCCCTAAAGAAAAAGTAGGTCTAGCAAATGGTATTTATGGTATGGGAAAT
    ATCGGTACAGCAGTTTCTTCATTTTTAGCACCACCGATAGCGGGTATTATTGGTTGGCAA
    ACAACAGTTAGAAGTTACTTAATTATTATCGCTTTATTTGCATTAATTATGTTCATTTTT
    GGTGACACACAAGAACGTAAAATTAAAGTACCATTAATGGCTCAAATGAAAACATTATCT
    AAAAACTACAAATTATATTACTTAAGTTATTGGTATTTTATTACTTTTGGTGCTTTTGTA
    GCATTTGGTATTTTCTTACCTAACTACTTAGTAAATCATTTTGGAATTGACAAAGTAGAT
    GCTGGTATTCGATCAGGTGTATTCATTGCGCTGGCAACATTCTTAAGACCAATAGGTGGC
    ATTTTAGGTGATAAATTTAATGCAGTTAAAGTATTGATGATTGATTTTGTTGTTATGATT
    ATCGGTGCCATTATTTTAGGTATTTCAGACCATATCGCATTATTCACTGTAGGCTGTTTA
    ACAATAAGTATTTGTGCAGGTATTGGTAACGGCTTAATCTTCAAATTAGTACCATCATAC
    TTCTTAAATGAAGCGGGATCCGCAAATGGTATCGTATCAATGATGGGTGGTTTAGGAGGA
    TTCTTCCCACCACTAGTAATCACGTACGTAGCTAATTTAACAGGATCAAGTCATTTAGCA
    TTTATTTTCTTAGCGGTATTTGGATGTATTGCATTATTTACCATGCGTCATTTATACCAA
    AAAGAATATGGCTCATTGAAAAACGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1170

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2333 [new locus tag: SAUSA300_RS12890 ]
  • symbol: NarK
  • description: nitrite extrusion protein
  • length: 389
  • theoretical pI: 9.92595
  • theoretical MW: 42148.2
  • GRAVY: 0.921851

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsnitrite transporter (TIGR00886; HMM-score: 197.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 58.2)
    MetabolismTransport and binding proteinsOthercyanate transporter (TIGR00896; HMM-score: 58)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 50.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 44.7)
    multidrug resistance protein (TIGR00880; HMM-score: 36.3)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 22.1)
    oxalate/formate antiporter (TIGR04259; HMM-score: 21.7)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 15.3)
  • TheSEED:  
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryNitrate and nitrite ammonification Nitrate/nitrite transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 150.3)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 19)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 11.5)
    MFS (CL0015) MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 8.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 36
    • Cmax: 0.127
    • Cmax_pos: 36
    • Smax: 0.291
    • Smax_pos: 27
    • Smean: 0.216
    • D: 0.19
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMASGFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDFVVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • narK no polycistronic organisation predicted

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]