NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• pan locus tag^?: SAUPAN005980000
• symbol: SAUSA300_2377
• pan gene symbol^?: —
• synonym:
• product: glycerate kinase

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• symbol: SAUSA300_2377
• product: glycerate kinase
• replicon: chromosome
• strand: +
• coordinates: 2556402..2557544
• length: 1143
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913966 NCBI
• RefSeq: YP_495012 NCBI
• MicrobesOnline: 1293892 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGCAATTACAAAAAATTGTCATCGCTCCTGACTCATTTAAGGAAAGTATGACCGCACAG
  CAAGTTGGCAATATTATAAAACAGGCTTTTACTAATGTTTATGGGAATACCCTTCATTAT
  GATATCATTCCGATGGCTGATGGTGGTGAAGGTACCACAGATGCTTTAATGCATGCAACA
  GGTGCCACTAAGTATACAGTCATCGTTAATGACCCTTTAATGCGACCTATTGAAGCATGT
  TATGCACGCGCAGACGAACAACAAATTGCAATTATTGAAATGGCGGCAGCGTCAGGTTTG
  GATTTATTAGAAAAAGAGGAACGTAATCCTTTATACACATCATCATATGGTACCGGTGAA
  CTAATTAAAGATGCATTAAATCATGGTGCTAAGACCATTATTTTAGGGATTGGTGGCAGT
  GCAACAAATGATGGTGGTACAGGTATGCTAAGTGCACTAGGCGTAAAGTTTACTGATGTA
  AACGGGGACTTATTACAAATGAATGGTGCTAATCTTGCTCACATTGCACAAATCGATATA
  ACCAATCTAGATTCGCGATTAAAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAAT
  CCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCA
  AAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGC
  ACAGGAAAGTCCGTTAATCAAATACCAGGTTCTGGTGCAGCTGGCGGTATGGGCGCAGCA
  TTATTAGCGTTTTGTGAGACAACTTTAACAAAAGGTATTGATGTCGTCTTTGACATTACA
  GATTTTCATCAAAGAATTAAAGATGCAGACCTCGTTATTACTGGAGAAGGACGCATGGAT
  TATCAGACCATCTTTGGTAAAACACCCGTAGGCGTTGCGTTAGCTGCAAAACAATATCAT
  ATTCCTGTCATCGCGATTTGTGGCAGTCTAGGCGAAAATTATCAACATGTTTACGATTTC
  GGTATTGATAGTGCCTATTCTATAATCTCTTCACCTAGCACTTTAGAAGATGTCCTACAA
  AATAGCGAACAAAATTTATTAAACACTGCAACTGACATTGCTCGTATTCTGAAATTACAA
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1143
  

⊟Protein

⊟General

• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• symbol: SAUSA300_2377
• description: glycerate kinase
• length: 380
• theoretical pI: 4.76396
• theoretical MW: 40660.1
• GRAVY: -0.02

⊟Function

• reaction:
  EC 2.7.1.31?  ExPASy
  Glycerate 3-kinaseATP + D-glycerate = ADP + 3-phospho-D-glycerate
• TIGRFAM:
  MetabolismEnergy metabolismOtherglycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 576.3)
• TheSEED:  
  Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Glycerate kinase (EC 2.7.1.31) 
  CarbohydratesMonosaccharidesD-galactarate, D-glucarate and D-glycerate catabolism - gjo Glycerate kinase (EC 2.7.1.31) 
  MonosaccharidesD-galactarate, D-glucarate and D-glycerate catabolism Glycerate kinase (EC 2.7.1.31) 
  One-carbon MetabolismSerine-glyoxylate cycle Glycerate kinase (EC 2.7.1.31) 
  Organic acidsGlycerate metabolism Glycerate kinase (EC 2.7.1.31) 
  Fatty Acids, Lipids, and IsoprenoidsPhospholipidsGlycerolipid and Glycerophospholipid Metabolism in Bacteria Glycerate kinase (EC 2.7.1.31) 
• PFAM:
  no clan definedGly_kinase; Glycerate kinase family (PF02595; HMM-score: 484.1)
  NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)
  no clan definedT6SS_VipA; Type VI secretion system, VipA, VC_A0107 or Hcp2 (PF05591; HMM-score: 11.6)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.146
  • Ymax_pos: 11
  • Cmax: 0.123
  • Cmax_pos: 25
  • Smax: 0.257
  • Smax_pos: 3
  • Smean: 0.227
  • D: 0.177
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161860 NCBI
• RefSeq: YP_495012 NCBI
• UniProt: A0A0H2XHY3 UniProt

⊟Protein sequence

• MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIPMADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDLLEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDVNGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGADAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVVFDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENYQHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2377 > SAUSA300_2378

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.