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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
  • pan locus tag?: SAUPAN006007000
  • symbol: pnbA
  • pan gene symbol?: pnbA
  • synonym:
  • product: para-nitrobenzyl esterase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
  • symbol: pnbA
  • product: para-nitrobenzyl esterase
  • replicon: chromosome
  • strand: +
  • coordinates: 2580823..2582175
  • length: 1353
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAGATTAATACTACAGGTGGTCAAATTCATGGTATTACACAAGATGGTTTAGATATC
    TTCTTAGGCATTCCTTATGCAGAACCACCAGTTCATGACAATCGCTTTAAACATTCTACG
    TTAAAAACACAATGGTCAGAGCCAATTGATGCAACTGAAATACAACCCATCCCACCGCAA
    CCAGACAACAAATTAGAAGATTTTTTCTCCTCACAATCTACAACTTTTACTGAACATGAA
    GACTGTTTATATCTAAATATTTGGAAACAACATAATGATCAGACGAAGAAACCTGTCATC
    ATTTATTTTTATGGTGGTAGTTTTGAAAATGGTCATGGTACAGCCGAACTCTATCAACCG
    GCACATTTAGTACAAAATAACGACATTATCGTTATTACATGCAATTATCGTTTAGGCGCA
    TTAGGATATTTAGACTGGTCATATTTTAATAAAGATTTTCATTCCAATAATGGCCTTTCA
    GATCAAATCAATGTCATAAAATGGGTGCATCAATTTATTGAATCCTTCGGTGGCGACGCT
    AATAACATTACTTTAATGGGTCAGTCTGCAGGCAGTATGAGCATTTTGACTTTACTTAAA
    ATACCTGACATTGAGCCATACTTCCATAAAGTCGTTCTACTAAGTGGCGCACTACGATTA
    GACACCCTTGAGAGTGCACGCAATAAAGCACAACATTTCCAAAAAATGATGCTCGATTAT
    TTAGATACAGATGATGTTACATCATTATCGACAAATGATATTCTTATGCTGATGGCGAAG
    CTAAAACAATCTCGAGGACCTTCTAAAGGGCTTGATTTAATATATGCGCCTATTAAAACA
    GATTATATACAAAATAATTATCCAACAACGAAACCAATTTTTGCATGTTATACAAAAGAT
    GAAGGCGATATTTATATTACTAGTGAACAGAAAAAATTATCGCCGCAACGCTTTATCGAC
    ATTATGGAATTAAATGATATTCCTTTAAAATACGAAGATGTTCAGACGGCGAAGCAACAA
    TCTTTAGCGATTACACATTGTTATTTCAAACAGCCGATGAAGCAATTTTTACAACAACTC
    AATATACAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCT
    AGCGCACACTATCGCAGTGCTTACCATATTCTAGATATGGTATTTTGGTTCGGAAACTTA
    CAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAA
    ATGCAAAATGATTTAGCTAATTTTGCTAAAAGCGGTAAAATGCCTTGGCCGATGTATCAT
    AATGAACGTCGCTATTATCGCACATATCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1353

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2396 [new locus tag: SAUSA300_RS13270 ]
  • symbol: PnbA
  • description: para-nitrobenzyl esterase
  • length: 450
  • theoretical pI: 6.50843
  • theoretical MW: 51968.5
  • GRAVY: -0.461111

Function[edit | edit source]

  • reaction:
    EC 3.1.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • Carboxylesterase type B (EC 3.1.1.-)
  • PFAM:
    AB_hydrolase (CL0028) COesterase; Carboxylesterase family (PF00135; HMM-score: 253.6)
    and 4 more
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 27.3)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 20.5)
    Say1_Mug180; Steryl acetyl hydrolase (PF10340; HMM-score: 17.6)
    SGNH_hydrolase (CL0264) ALGX; SGNH hydrolase-like domain, acetyltransferase AlgX (PF16822; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01266
    • TAT(Tat/SPI): 0.001294
    • LIPO(Sec/SPII): 0.002138
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQWSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIYFYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLSDQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGALRLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYAPIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDVQTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILDMVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]