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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02500 [old locus tag: SAUSA300_0466 ]
  • pan locus tag?: SAUPAN002225000
  • symbol: SAUSA300_RS02500
  • pan gene symbol?: rsmI
  • synonym:
  • product: rRNA (cytidine-2'-O-)-methyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02500 [old locus tag: SAUSA300_0466 ]
  • symbol: SAUSA300_RS02500
  • product: rRNA (cytidine-2'-O-)-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 524664..525503
  • length: 840
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGTATTATATTTAGTGGGCACACCAATTGGTAATTTAGCAGATATTACTTATAGA
    GCAGTTGATGTATTGAAACGTGTTGATATGATTGCTTGTGAAGACACTAGAGTAACTAGT
    AAACTGTGTAATCATTATGATATTCCAACTCCATTAAAGTCATATCACGAACATAACAAG
    GATAAGCAGACTGCTTTTATCATTGAACAGTTAGAATTAGGTCTTGACGTTGCGCTCGTA
    TCTGATGCTGGATTGCCCTTAATTAGTGATCCTGGATACGAATTAGTAGTGGCAGCCAGA
    GAAGCTAATATTAAAGTAGAGACCGTGCCTGGACCTAATGCTGGGCTGACGGCTTTGATG
    GCTAGTGGATTACCTTCATATGTATATACATTTTTAGGATTTTTGCCACGAAAAGAGAAA
    GAAAAAAGTGCTGTATTAGAGCAACGTATGCATGAAAATAGCACATTAATTATATACGAA
    TCACCGCATCGTGTGACAGATACATTAAAAACAATTGCAAAGATAGATGCAACACGACAA
    GTATCACTAGGGCGTGAATTAACTAAGAAGTTCGAACAAATTGTAACTGATGATGTAACA
    CAATTACAAGCATTGATTCAGCAAGGCGATGTACCATTGAAAGGCGAATTCGTTATCTTA
    ATTGAAGGTGCTAAAGCGAACAATGAGATATCGTGGTTTGATGATTTATCTATCAATGAG
    CATGTTGATCATTATATTCAAACTTCACAGATGAAACCAAAACAAGCTATTAAAAAAGTT
    GCTGAAGAACGACAACTTAAAACGAATGAAGTATATAATATTTATCATCAAATAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS02500 [old locus tag: SAUSA300_0466 ]
  • symbol: SAUSA300_RS02500
  • description: rRNA (cytidine-2'-O-)-methyltransferase
  • length: 279
  • theoretical pI: 5.62169
  • theoretical MW: 31274.6
  • GRAVY: -0.208961

Function[edit | edit source]

  • reaction:
    EC 2.1.1.198?  ExPASy
    16S rRNA (cytidine1402-2'-O)-methyltransferase S-adenosyl-L-methionine + cytidine1402 in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (TIGR00096; EC 2.1.1.198; HMM-score: 274.2)
    and 6 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-4 C11-methyltransferase (TIGR01465; EC 2.1.1.133; HMM-score: 25.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen-III C-methyltransferase (TIGR01469; EC 2.1.1.107; HMM-score: 23.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-2 C(20)-methyltransferase (TIGR01467; EC 2.1.1.130; HMM-score: 20.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-3B C17-methyltransferase (TIGR01466; EC 2.1.1.131; HMM-score: 17.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogenase MoFe cofactor biosynthesis protein NifE (TIGR01283; HMM-score: 14.1)
  • TheSEED: see SAUSA300_0466
  • PFAM:
    no clan defined TP_methylase; Tetrapyrrole (Corrin/Porphyrin) Methylases (PF00590; HMM-score: 116.7)
    and 2 more
    Hexosaminidase (CL0546) Glycohydro_20b2; beta-acetyl hexosaminidase like (PF14845; HMM-score: 15.4)
    P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 12.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025341
    • TAT(Tat/SPI): 0.000726
    • LIPO(Sec/SPII): 0.004956
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCNHYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREANIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYESPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFVILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYHQIS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]