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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
  • pan locus tag?: SAUPAN002857000
  • symbol: SAUSA300_RS04365
  • pan gene symbol?:
  • synonym:
  • product: primase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
  • symbol: SAUSA300_RS04365
  • product: primase
  • replicon: chromosome
  • strand: +
  • coordinates: 887405..889777
  • length: 2373
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007793 (887405..889777) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    GTGGCAATTAAGAAAAAAGATAGAATTATTGGAGTTAAAGAATTAGAAATACCGCAAGAA
    TTAAAATTAGTGCCCAATTGGGTATTATGGCGAGCTGAATGGAACGAGAAGCAACAAAAT
    TTTGGAAAAGTACCATATAGTATTAATGGTTACAGAGCTAGTACAACCAATAAAAAAACA
    TGGTGTGACTTTGAAAGTGTAAGTATTGAATATGAAGTTGATGAGCAATATAGCGGTATA
    GGTTTTGTATTAAGTGATGGTAATAATTTTGTTTGCCTAGATATTGATAATGCAATTGAT
    AAAAAAGGACAAATCAATTCTGAATTAGCACTAAAAATGATGCAACTCACATATTGTGAA
    AAGTCTCCAAGTGGTACAGGATTACATTGTTTCTTTAAAGGTAAACTACCAGATAACCGT
    AAAAAGAAAAGAACAGATTTAGACATAGAGTTATATGATTCAGCAAGGTTTATGACTGTT
    ACAGGATGCACAATTGGTCAAAGTGATATTTGTGATAATCAAGAAGTATTAAATACTCTA
    GTTGATGAATATTTTAAAGAGAATTTGCCAGCAAATGAGGTTGTGAGAGAGGAATCTAAT
    ACCAATATACAATTATCTGATGAAGATATTATAAACATTATGATGAAATCTAAACAAAAA
    GATAAAATTAAAGATCTATTACAAGGCACATATGAATCATATTTTGAGAGTTCAAGCGAA
    GCAGTACAAAGCTTATTACACTACTTAGCGTTTTACACAGGTAAAAACAAACAGCAAATG
    GAGCGTATATTTTTAAACTACAATAATCTTACGGATAAATGGGAAAGTAAACGAGGTAAT
    ACGACTTGGGGACAGCTTGAGTTAGATAAAGCTATAAAGAATCAAAAGACAATTTATACT
    AAATCGATAGATGAATTTAATGTTATACCACAGGGGAGTAAAGATGTTAAACAGTTGTTG
    AATCAATTGGGGCATGAAGAAAGAACAAAAATGGAAGAAAATTGGATTGAAGAAGGAAAA
    CGAGGGCGAAAGCCTACAACAATTAGCCCTATAAAATGTGCATATATTTTGAATGAGCAT
    TTAACATTTATACTTTTTGATGATGAAGAAAATACTAAGTTAGCTATGTATCAATTTGAT
    GAAGGGATATATACACAGAACACTACAATTATAAAACGAGTGATTTCCTATTTAGAGCCC
    AAACATAATAGCAACAAAGCTGATGAAGTTATTTATCATTTAACCAATATGGTAGATATA
    AAAGAGAAAACTAACTCACCATACTTAATACCAGTTAAAAATGGTGTATTTAACCGTAAA
    ACGAAGCAACTAGAATCATTTACACCTGATTATATATTTACCAGTAAAATAGATACATCG
    TATGTAAGGCAAGATATAGTACCTGAAATAAATGGCTGGAATATAGATCGGTGGATAGAA
    GAGATTGCTTGTAATGATAATCAGGTTGTTAAATTATTGTGGCAAGTAATTAATGACTCA
    ATGAATGGTAACTACACTCGTAAAAAGGCTATTTTCTTTGTTGGTGATGGAAATAATGGT
    AAAGGGACGTTTCAAGAATTATTGTCTAATGTAATAGGTTATAGCAATATTGCTAGCTTA
    AAAGTGAATGAGTTTGATGAACGTTTTAAATTGAGTGTGTTAGAGGGCAAGACAGCAGTA
    ATTGGAGACGATGTACCAGTTGGTGTGTATGTCGATGATTCTTCAAACTTTAAAAGTGTA
    GTTACTGGCGATCCAGTGTTGGTTGAGTTTAAAAATAAACCCTTATATAGAGCGACTTTT
    AAGTGTACAGTTATTCAATCAACGAATGGAATGCCTAAATTTAAAGACAAAACAGGCGGG
    ACTTTAAGAAGGTTATTGATAGTACCGTTTAATGCCAATTTTAATGGCATTAAAGAGAAT
    TTTAAAATCAAAGAAGACTATATAAAAAATCAGCAAGTGCTAGAGTATGTGCTTTATAAA
    GCAATTAATTTAGATTTTGAAACCTTTGACATTCCTGATGCATCTAAAAAAATGCTTGAA
    GTATTTAAAGAAGATAACGATCCAGTTTATGGGTTTAAAGTAAATATGTTTGATCAATGG
    ACTATTAGAAAAGTGCCGAAATATATTGTATACGCATTTTATAAAGAATATTGTGATGAA
    AATGGCTATAATGCATTGAGTTCAAACAAGTTTTATAAACAATTTGAACATTATTTAGAG
    AATTATTGGAAAACTGATGCACAGCGAAGATATGACAATGAAGAACTTGCTAAGAGGATA
    TACAACTTTAATGACAATAGAAATTACATTGAACCTATTGAAAGTGGAAAAAACTATAAA
    TCGTATGAAAAGGTGAAGCTAAAAGCAATATAG
    60
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    2373

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS04365 [old locus tag: SAUSA300_0809 ]
  • symbol: SAUSA300_RS04365
  • description: primase
  • length: 790
  • theoretical pI: 6.08403
  • theoretical MW: 92004.7
  • GRAVY: -0.631646

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairphage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 370.7)
    HipA N-terminal domain (TIGR03071; HMM-score: 14.1)
  • TheSEED:
  • PFAM:
    HTH (CL0123) Pox_D5; Poxvirus D5 protein-like (PF03288; HMM-score: 54.3)
    no clan definedD5_N; D5 N terminal like (PF08706; HMM-score: 39.9)
    HTH (CL0123) RFX_DNA_binding; RFX DNA-binding domain (PF02257; HMM-score: 16.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.119
    • Ymax_pos: 11
    • Cmax: 0.113
    • Cmax_pos: 33
    • Smax: 0.217
    • Smax_pos: 1
    • Smean: 0.127
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446907979 NCBI
  • RefSeq: WP_000985235 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKVPYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKKGQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTVTGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSKQKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWESKRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEENWIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIKRVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPDYIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRKKAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVPVGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRRLLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVFKEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLENYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS04365 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]