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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04740 [old locus tag: SAUSA300_0879 ]
  • pan locus tag?: SAUPAN003116000
  • symbol: SAUSA300_RS04740
  • pan gene symbol?:
  • synonym:
  • product: 2-isopropylmalate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS04740 [old locus tag: SAUSA300_0879 ]
  • symbol: SAUSA300_RS04740
  • product: 2-isopropylmalate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 966420..967565
  • length: 1146
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007793 (966420..967565) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATTCGAATTCAAGATAATACAATAAGGGATGGTATGCAACAAAGTAATGTTCGAAAA
    AGTCTAATTATAAAAAAAGAAGTATTGAAACAAATTAACAAGTTAAATATAAATTCTGTT
    GAAGTAGGCATGTGTACAACTATCGAGGATGAATTTAATATTCATCAATTCAGAGACATT
    TTAAGTCCTGAAAAAGAATTAGTAGTATTGACTAGGCTTAATGAAAAAGAAATAAAAAAA
    ATAGTCAAATTGAAAATTCATAATTTAGTGGTAAAAATACTATTGCCAATATCTGACTTG
    CATATAAAAGAAAAGCTTAATTTTTCAAATAAATATTATATTCAGAAAATCAAAGACTGC
    TTGGATATATTAAAGAAAGATAAAAAAGGAGTAGATATTTGTTTTGAAGATGGAACAAGG
    ACTTCTAGAGAAAAATTGAAAGAATACATGGAAATTATTTCAAAATATCAAGTTAGAACA
    GTTACATTTGCGGACACTGTAGGATGTTCGACACCATTAGAATACGGAGATATTTTTAAT
    TACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGG
    TTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACA
    TTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTG
    ACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACTAGTATT
    AATATTTCTAAAATTCTCGATTTTCAAATATCAGAAAACAAACCTATAATTGGTGAAAAT
    ATATTTAAACATGAATCAGGAATTCATCAAGATGGTACTAAAAAAAATATAAATATGTAT
    CAATATTTAGTTCCTAGTGATTTAGGATTGAAAAATTCACAAGTTGTTCAGTTTCCAATA
    AGTAATATTTCTAGTAAGAAAATCTTGCACAATAAATTTAAATCAATAGTTAACACTGAA
    GAAATTGATGAAAATATTTCTTTCTATAAACTTGTGAACAAGTTTTCACCTGAAGTAGCA
    CCTGAAGATACAGTGGATTTACTTCAGATAATAAAAAGGAGGAGTAAAGATGGAAATTTT
    AAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS04740 [old locus tag: SAUSA300_0879 ]
  • symbol: SAUSA300_RS04740
  • description: 2-isopropylmalate synthase
  • length: 381
  • theoretical pI: 9.29629
  • theoretical MW: 43773.5
  • GRAVY: -0.320735

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 222.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 217.3)
    MetabolismCentral intermediary metabolismNitrogen fixationhomocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 217.3)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 197.4)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 168.5)
    Unknown functionGeneralcitramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 105)
    MetabolismEnergy metabolismOther4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 61.1)
    MetabolismAmino acid biosynthesisPyruvate family2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 52.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsoxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.6)
    MetabolismEnergy metabolismOtheroxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 26.6)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 184.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 38
    • Cmax: 0.105
    • Cmax_pos: 51
    • Smax: 0.206
    • Smax_pos: 30
    • Smean: 0.121
    • D: 0.123
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446542020 NCBI
  • RefSeq: WP_000619366 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS04740 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]