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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
  • pan locus tag?: SAUPAN003237000
  • symbol: SAUSA300_RS05055
  • pan gene symbol?:
  • synonym:
  • product: ABC transporter substrate-binding protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
  • symbol: SAUSA300_RS05055
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1029745..1030704
  • length: 960
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGAATAGGAATATCGTTAAACTAGTTGTGTTCATGCTAATTTTAGTTGTAGCAGTAGCG
    GGTTGTGGTCAAAAAGATACTGAAGAGAAAACTGAAATGACGACAATAAAAGATGAATTA
    GGAACTGAAAAAATTAAGAAAAATCCTAAACGTGTTGTTGTATTAGAATATAGTTTTGCT
    GATTATTTAGCAGCATTAGATATGAAACCTGTTGGTATTGCAGATGATGGCAGCACTAAA
    AATATAACAAAGTCAGTAAGAGATAAGATTGGGGCATATGAATCGGTTGGATCTAGACCG
    CAACCGAATATGGAAGTGATAAGTAAATTAAAACCGGATTTGATCATTGCAGATGTTAGC
    AGACATAAGAAAATCAAATCAGAATTGAGCAAAATTGCTCCGACAATCATGTTAGTTAGC
    GGTACGGGAGATTATAATGCAAATATTGAAGCATTTAAAACAGTCGCTAAAGCAGTAGGC
    AAAGAGAAAGAAGGCGAGAAGCGTCTGGAAAAGCATGATAAAATATTAGCGGAGATTAGA
    AAGAAAATTGAACAGAGTACGTTAAAATCTGCATTTGCATTCGGTATCTCAAGAGCAGGT
    ATGTTTATTAATAATGAAGATACATTTATGGGACAATTCTTAATTAAAATGGGTATTCAA
    CCTGAAGTCACAAAAGACAAAACTACGCATGTTGGTGAACGCAAGGGTGGTCCTTATATA
    TATTTAAATAATGAAGAACTTGCCAATATCAATCCAAAAGTTATGATTTTAGCCACTGAC
    GGAAAAACGGACAAAAATAGAACGAAATTCATTGATCCTGCAGTTTGGAAATCATTAAAA
    GCTGTGAAAGATAACAAAGTTTATGACGTTGACCGAAATAAGTGGTTGAAATCAAGGGGG
    ATTATCGCAAGTGAAAGTATGGCAGAAGATTTAGAAAAAATTGCAGAAAAAGCAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS05055 [old locus tag: SAUSA300_0941 ]
  • symbol: SAUSA300_RS05055
  • description: ABC transporter substrate-binding protein
  • length: 319
  • theoretical pI: 10.0458
  • theoretical MW: 35566.2
  • GRAVY: -0.410658

Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 92.9)
    and 2 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 30.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lipoprotein, PulS/OutS family (TIGR01004; HMM-score: 11.8)
  • TheSEED: see SAUSA300_0941
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 115.9)
    and 2 more
    LppaM (CL0421) LPAM_2; Prokaryotic lipoprotein-attachment site (PF13627; HMM-score: 15.2)
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 14.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.81
    • Cellwall Score: 0.09
    • Extracellular Score: 0.1
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 21 aa
    • SP(Sec/SPI): 0.000485
    • TAT(Tat/SPI): 0.000074
    • LIPO(Sec/SPII): 0.999197
    • Cleavage Site: CS pos: 21-22. VAG-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEKIKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQPNMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVGKEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMGIQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVWKSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]