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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS05405 [old locus tag: SAUSA300_1004 ]
  • pan locus tag?: SAUPAN003329000
  • symbol: SAUSA300_RS05405
  • pan gene symbol?:
  • synonym:
  • product: membrane protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS05405 [old locus tag: SAUSA300_1004 ]
  • symbol: SAUSA300_RS05405
  • product: membrane protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1099705..1100124
  • length: 420
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAATAGAAAACCTGAATTAATCATGGCTTGGATTGCAAATAGTATTAGTATTATTTAT
    TTATTAGTTATGGGGCTATCCTATTTTTCTTTAAAAAGTGGTAATGCATCACAACGTGAA
    GAATTAGCGAAGCAATTATCTCAGAACGGTGGCAAGGTTTCTTTAGATATGCTTCAGACA
    ACAATGGGTGCATTAGCAATTATTTTATTAATTTCAACACTTTATGGTATATTTGCGACA
    ATTTGTATTAAAGGACGTAGAAAATTATCGATTATACTTTTTGTTATCGCGATAATTGTA
    AGTTTGATGGCTCTTAATTTAATTGCAATTGTCTTATGGGTTATCGTGATGATTATGTTG
    ATTTCTAAAAAAGAATCAAAAGAAACAACACATAAGGACGATGAGTATATTTATCATTAA
    60
    120
    180
    240
    300
    360
    420

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS05405 [old locus tag: SAUSA300_1004 ]
  • symbol: SAUSA300_RS05405
  • description: membrane protein
  • length: 139
  • theoretical pI: 9.90462
  • theoretical MW: 15576.8
  • GRAVY: 0.682014

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides oligosaccharide repeat unit polymerase (TIGR04370; HMM-score: 15.2)
  • TheSEED: see SAUSA300_1004
  • PFAM:
    Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 71.6)
    and 13 more
    no clan defined GWT1; GWT1 (PF06423; HMM-score: 17.8)
    DUF2663; Protein of unknown function (DUF2663) (PF10864; HMM-score: 15.1)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 13.8)
    no clan defined P2X_receptor; ATP P2X receptor (PF00864; HMM-score: 11.1)
    Ninjurin; Ninjurin (PF04923; HMM-score: 11.1)
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 9.6)
    EphA2_TM; Ephrin type-A receptor 2 transmembrane domain (PF14575; HMM-score: 8.9)
    Tmemb_9; TMEM9 (PF05434; HMM-score: 8)
    DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 7.4)
    TMEM154; TMEM154 protein family (PF15102; HMM-score: 7.2)
    Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 6.6)
    Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 6.4)
    no clan defined PRA1; PRA1 family protein (PF03208; HMM-score: 6.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.156691
    • TAT(Tat/SPI): 0.003661
    • LIPO(Sec/SPII): 0.031929
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAKQLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSLMALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]