NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS07475 [old locus tag: SAUSA300_1369 ]
• pan locus tag^?: SAUPAN003944000
• symbol: SAUSA300_RS07475
• pan gene symbol^?: —
• synonym:
• product: thioredoxin reductase

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS07475 [old locus tag: SAUSA300_1369 ]
• symbol: SAUSA300_RS07475
• product: thioredoxin reductase
• replicon: chromosome
• strand: -
• coordinates: 1535678..1536664
• length: 987
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (1535678..1536664) NCBI
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGCAAAAAGTTGAAAGTATCATAATTGGTGGAGGGCCATGCGGATTAAGTGCGGCTATT
  GAACAAAAAAGAAAAGGTATTGATACCTTAATTATTGAAAAGGGTAATGTCGTTGAATCA
  ATCTACAATTATCCTACTCACCAAACATTTTTCTCATCAAGTGATAAATTAAGTATTGGG
  GACGTACCGTTTATCGTTGAAGAAAGTAAACCAAGACGTAATCAAGCGCTAGTTTATTAC
  CGAGAAGTTGTAAAACATCATCAATTAAAAGTAAATGCATTTGAAGAAGTATTAACTGTT
  AAAAAAATGAATAATAAATTTACTATTACTACGACGAAAGATGTTTATGAATGTCGATTT
  TTAACAATCGCGACAGGCTATTATGGTCAGCATAATACATTAGAAGTTGAAGGTGCGGAT
  TTACCTAAAGTGTTCCATTATTTTAAAGAGGCACATCCGTATTTTGATCAAGATGTTGTA
  ATTATCGGTGGTAAGAATTCGGCTATCGATGCTGCTTTGGAGTTGGAAAAAGCTGGTGCT
  AACGTGACGGTTCTATATCGTGGTGGAGATTATTCGCCTTCAATTAAACCGTGGATACTT
  CCAAATTTCACAGCATTAGTAAATCATGAAAAAATTGACATGGAATTTAATGCTAATGTT
  ACCCAAATAACTGAAGATACTGTGACTTATGAAGTAAATGGTGAAAGTAAAACGATACAC
  AATGATTATGTATTTGCGATGATTGGTTATCATCCCGATTATGAATTTTTAAAATCTGTA
  GGCATTCAAATTAATACAAATGAATTTGGAACAGCGCCTATGTATAATAAAGAAACATAC
  GAAACAAATATCGAAAATTGCTATATTGCAGGTGTAATTGCTGCAGGGAACGATGCGAAT
  ACCATTTTTATTGAAAATGGTAAATTCCACGGGGGCATTATTGCTCAAAGCATGCTAGCT
  AAGAAACAAACGCCCTTAGAATCATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  987
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS07475 [old locus tag: SAUSA300_1369 ]
• symbol: SAUSA300_RS07475
• description: thioredoxin reductase
• length: 328
• theoretical pI: 5.39888
• theoretical MW: 36736.3
• GRAVY: -0.271646

⊟Function

• reaction:
  EC 1.8.1.9?  ExPASy
  Thioredoxin-disulfide reductaseThioredoxin + NADP⁺ = thioredoxin disulfide + NADPH
• TIGRFAM:
  Unknown functionEnzymes of unknown specificityputative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 457.2)
  MetabolismEnergy metabolismElectron transportthioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 87.3)
  Cellular processesCellular processesDetoxificationCoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 61.7)
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 59.3)
  Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 50.8)
  Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 50.8)
  Cellular processesCellular processesDetoxificationmercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 49.4)
  putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 48.8)
  putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 48.2)
  MetabolismAmino acid biosynthesisGlutamate familyglutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 38.6)
  flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 35.1)
  Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 34.1)
  MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 29.5)
  squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 25.6)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis enzyme (TIGR00292; HMM-score: 25.5)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 25.2)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 23.3)
  MetabolismEnergy metabolismOther4-hydroxybenzoate 3-monooxygenase (TIGR02360; EC 1.14.13.2; HMM-score: 20.8)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin 3B synthase CobZ (TIGR02485; HMM-score: 20.3)
  pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 20.2)
  mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 19.2)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 19)
  MetabolismCentral intermediary metabolismNitrogen metabolismnitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 18.4)
  glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 18.2)
  MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 18.1)
  MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 17.1)
  MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, monomeric form (TIGR01377; HMM-score: 16.8)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 16.8)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherphytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 16.7)
  MetabolismEnergy metabolismTCA cyclesuccinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 16.4)
  MetabolismEnergy metabolismAnaerobicsuccinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 16.4)
  MetabolismEnergy metabolismAerobicsuccinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 16.4)
  thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 16)
  lycopene cyclase family protein (TIGR01790; HMM-score: 15.8)
  MetabolismEnergy metabolismTCA cyclesuccinate dehydrogenase, flavoprotein subunit (TIGR01816; HMM-score: 15)
  MetabolismEnergy metabolismElectron transportflavocytochrome c (TIGR01813; HMM-score: 14.9)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02028; HMM-score: 12.8)
  FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 12.4)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 12.2)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 11.2)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesL-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 11.1)
  Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 9.4)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamineglycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 8)
• TheSEED:
• PFAM:
  NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 328)
  Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 92.7)
  Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 39.6)
  FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 38.6)
  FAD_binding_3; FAD binding domain (PF01494; HMM-score: 35.4)
  FAD_binding_2; FAD binding domain (PF00890; HMM-score: 34.1)
  HI0933_like; HI0933-like protein (PF03486; HMM-score: 30.7)
  Thi4; Thi4 family (PF01946; HMM-score: 28.2)
  GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 25.9)
  NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 24.8)
  Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 17.3)
  FMO-like; Flavin-binding monooxygenase-like (PF00743; HMM-score: 16.9)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.7)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 16.2)
  ACT (CL0070) ALS_ss_C; Small subunit of acetolactate synthase (PF10369; HMM-score: 11.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.138
  • Ymax_pos: 27
  • Cmax: 0.129
  • Cmax_pos: 19
  • Smax: 0.253
  • Smax_pos: 18
  • Smean: 0.133
  • D: 0.136
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447097004 NCBI
• RefSeq: WP_001174260 NCBI
• UniProt:

⊟Protein sequence

• MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNYPTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKKMNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVVIIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNANVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYNKETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS07475 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.