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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10540 [old locus tag: SAUSA300_1922 ]
  • pan locus tag?: SAUPAN005023000
  • symbol: SAUSA300_RS10540
  • pan gene symbol?: sak
  • synonym:
  • product: staphylokinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10540 [old locus tag: SAUSA300_1922 ]
  • symbol: SAUSA300_RS10540
  • product: staphylokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2088425..2088916
  • length: 492
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGCTCAAAAGAGGTTTATTATTTTTAACTGTTTTATTGTTATTATTCTCATTTTCTTCA
    ATTACTAATGAGGTAAGTGCATCAAGTTCATTCGACAAAGGAAAATATAAAAAAGGCGAT
    GACGCGAGTTATTTTGAACCAACAGGCCCGTATTTGATGGTAAATGTGACTGGAGTTGAT
    GGTAAAGGAAATGAATTGCTATCCCCTCATTATGTCGAGTTTCCTATTAAACCTGGGACT
    ACACTTACAAAAGAAAAAATTGAATACTATGTCGAATGGGCATTAGATGCGACAGCATAT
    AAAGAGTTTAGAGTAGTTGAATTAGATCCAAGCGCAAAGATCGAAGTCACTTATTATGAT
    AAGAATAAGAAAAAAGAAGAAACGAAGTCTTTCCCTATAACAGAAAAAGGTTTTGTTGTC
    CCAGATTTATCAGAGCATATTAAAAACCCTGGATTCAACTTAATTACAAAGGTTATTATA
    GAAAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    492

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS10540 [old locus tag: SAUSA300_1922 ]
  • symbol: SAUSA300_RS10540
  • description: staphylokinase
  • length: 163
  • theoretical pI: 7.63429
  • theoretical MW: 18474.1
  • GRAVY: -0.302454

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SAUSA300_1922
  • PFAM:
    Ubiquitin (CL0072) Staphylokinase; Staphylokinase/Streptokinase family (PF02821; HMM-score: 111.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.976782
    • TAT(Tat/SPI): 0.001652
    • LIPO(Sec/SPII): 0.019831
    • Cleavage Site: CS pos: 27-28. VSA-SS. Pr: 0.8969
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASYFEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKEFRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVIIEKK

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]