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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11495 [old locus tag: SAUSA300_2088 ]
  • pan locus tag?: SAUPAN005430000
  • symbol: SAUSA300_RS11495
  • pan gene symbol?: luxS
  • synonym:
  • product: S-ribosylhomocysteine lyase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11495 [old locus tag: SAUSA300_2088 ]
  • symbol: SAUSA300_RS11495
  • product: S-ribosylhomocysteine lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 2251700..2252170
  • length: 471
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACAAAAATGAATGTTGAAAGTTTCAATTTAGATCATACTAAGGTGGTTGCCCCATTT
    ATTAGATTAGCGGGAACGATGGAAGGATTAAACGGAGATGTCATTCACAAATACGACATT
    CGTTTCAAACAACCAAACAAAGAACATATGGATATGCCCGGACTACATTCATTAGAACAT
    TTAATGGCTGAAAATATTAGAAATCATAGTGACAAAGTTGTTGATTTAAGTCCTATGGGT
    TGCCAAACTGGTTTCTATGTATCATTTATTAATCATGATAATTATGATGATGTATTAAAT
    ATTGTTGAAGCAACTTTAAATGATGTGCTAAATGCTACTGAAGTGCCTGCTTGTAATGAA
    GTACAATGTGGCTGGGCAGCAAGTCATTCATTAGAAGGTGCTAAAACTATCGCTCAAGCA
    TTTCTAGACAAACGAAACGAATGGCATGATGTTTTCGGTACAGGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS11495 [old locus tag: SAUSA300_2088 ]
  • symbol: SAUSA300_RS11495
  • description: S-ribosylhomocysteine lyase
  • length: 156
  • theoretical pI: 5.45928
  • theoretical MW: 17513.7
  • GRAVY: -0.355128

Function[edit | edit source]

  • reaction:
    EC 4.4.1.21?  ExPASy
    S-ribosylhomocysteine lyase S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
    EC 3.13.1.-?  ExPASy
  • TIGRFAM:
  • TheSEED: see SAUSA300_2088
  • PFAM:
    Peptidase_ME (CL0094) LuxS; S-Ribosylhomocysteinase (LuxS) (PF02664; HMM-score: 186.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Fe cation
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005651
    • TAT(Tat/SPI): 0.000362
    • LIPO(Sec/SPII): 0.001327
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQPNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIVEATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]