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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13030 [old locus tag: SAUSA300_2360 ]
  • pan locus tag?: SAUPAN005949000
  • symbol: SAUSA300_RS13030
  • pan gene symbol?: mdeA
  • synonym:
  • product: drug:proton antiporter

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13030 [old locus tag: SAUSA300_2360 ]
  • symbol: SAUSA300_RS13030
  • product: drug:proton antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2535779..2537182
  • length: 1404
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTGATGGGTGGATTTTTTGGATTATTAAATGAAACACTATTAGTGACGGCTTTACCA
    AGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTT
    TTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACA
    AGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTG
    AGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCGTTAGGCGCAGGTATT
    ATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCACATGAACGCGGTAAA
    TATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCA
    GGTTACCTTGTTGAATATTTTAACTGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCA
    GCTGTGACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGAACTACAATTAAAGTA
    CCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGA
    ACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGA
    GGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTATCAACACCATTATTA
    AATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATTATTATGGGTGTCACA
    ATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCAT
    CGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATG
    TCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAATCTTGCTTTAGTAGGT
    ATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGATGAACAAACATCAACA
    ATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCA
    GTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACATGGTTCATCTATG
    ACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCATTAATTACTATTTTA
    TCCCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAATGGCATGAACAAAATT
    GATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTA
    TTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGTAAGAAA
    AAAGAAGTTGATTCTCGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1404

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS13030 [old locus tag: SAUSA300_2360 ]
  • symbol: SAUSA300_RS13030
  • description: drug:proton antiporter
  • length: 467
  • theoretical pI: 9.76001
  • theoretical MW: 50784.4
  • GRAVY: 0.775803

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 336.4)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 336.4)
    and 16 more
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 94.3)
    multidrug resistance protein (TIGR00880; HMM-score: 81.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 49.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 48.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 47.2)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 34.1)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 26.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 26.5)
    oxalate/formate antiporter (TIGR04259; HMM-score: 26.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids monocarboxylate transporter (TIGR00892; HMM-score: 23.3)
    Metabolism Transport and binding proteins Anions nitrite transporter (TIGR00886; HMM-score: 22.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 19.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 17.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids polyol permease (TIGR00897; HMM-score: 14.1)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 7)
    sodium-independent organic anion transporter (TIGR00805; HMM-score: 6.5)
  • TheSEED: see SAUSA300_2360
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 180.5)
    and 2 more
    MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 31.9)
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006867
    • TAT(Tat/SPI): 0.000203
    • LIPO(Sec/SPII): 0.010616
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]