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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 23-MAY-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030018 [new locus tag: SAUSA300_RS14825 ]
  • pan locus tag?:
  • symbol: traI
  • pan gene symbol?:
  • synonym:
  • product: transfer complex protein TraI (DNA topoisomerase III)

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_pUSA030018 [new locus tag: SAUSA300_RS14825 ]
  • symbol: traI
  • product: transfer complex protein TraI (DNA topoisomerase III)
  • replicon: pUSA03
  • strand: +
  • coordinates: 17414..19516
  • length: 2103
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAACACTTTAATTCTATGTGAAAAACCTTCACAAGCAATGGACTTAAGCACTGTTTTT
    GCAAAAAAGAAAAAGCAAAATGGATATATGGAAATTTCAGATGAACAATTAAATGTAAGT
    GGCTTTTTAACTTGGGCAGTTGGTCATTTAGTCGAATTGAAGGAACCACAAGAATATGAC
    GAAAAATATAAAAATTTTAGCACATATCCTATATTGTTAGAAAAAGACGATTTTCAATTT
    AAAGTATCTGACAAAACGAAAGATCAATTTAACAACATTAAAAAAATATTTAAAGAAAAC
    AAGATTGATGAAGTTATTATTGCTACTGACCCAGCTAGAGAAGGCGAAAATATAGCATAT
    AAAATTTTAAATCAATTGAAAGTAACAGATAAAGTTACTATAAAAAGATTGTGGCTTACT
    TCTAAAGTAGAAAGCAGTATAAGAAAGGCTTTTAAAAATATATTACCTAAAGAAAAAACT
    TATGGATTTTACAAAGAGGGCAGGGCTAGAGAATTAAGTGATTGGTTAGTAGGAATTAAC
    TTATCACGTCATTTCACTAAAATTTCACGAGAATTAGGAAATGACGGTGTTATTCATATC
    GGTAGAGTATCTAGTCCTACATTAAACATGGTTTACAACAGAGAGAATAACATTAAAGGT
    TTTAAAGGTAAAAAGTTTTATAAAGTTTCAGCGACTATTAACAAAGATGAACAAGAAGTG
    AAAACAGAGTTAAAAAACAAATTTGACAGTGAAGATGAATTGCACGAATTTTTGTTTGAA
    AATGATATTACAGATTTAACACAAAAAGGGTTAGTTACTGATATTGAAAAAGAAATAGGC
    TATACAATGCCACCTAAATTTTATGACTTATCAGCTTTACAAGAAGATATGAATGATAAA
    TATAAAATAAGTGCGAAACGAACATTAGAAATCGCACAGACATTATATGAGAAGAAATTA
    ATCACATATCCAAGAACTGATTCAAGATATATCACAGAAGATGAAAAAGAAATGTTATTA
    GAAAATATAGACTATTTAAAAGAAATAACAAAGATTAATTTGAATAATGAATTAACTAAT
    AACAGCTTGATTAATCCTTCTAAAATAGAAGATCATTACGCAATATTAATAACTGGAAAT
    GATTTTAACAAAGTAGATTTAAAAGAAGAAGAAATCAATGTTTATAAAAGTATATTACAA
    AATGTGGCCATGAATTTTATGGATAAAGAACAATATGAAACGACTACTATAGAAATCGCT
    GTTAAAAAGCTTATGTTTGAAGTGAAAGGTAAAATTATTCAAGATAACGGATTTAAAGCT
    TTGTTAAATAAACAAAAAACTTCCGAAGAAACAATTCCTAATTTTGAAAAAAATGAAGAA
    GTAGACATTGAATTAGATTTGTTAGAAAAAGAAACGACACCTCCAAAAAGATATACAGAA
    AAAACATTGTTAAAAGCAATGGCCAATCCTATAGAAACACTAGAAGATGAAGGCTTAAAG
    TCTACACTTAAAGAAGTTAAAGGTTTAGGCACACCAGCAACTAGAGCCGATATTATAGAA
    AACTTGAAGAAAAATAAATATATTCAAGTACAAAAAAACAAAATTTATATTACTAAAAAT
    GGTATATTAGCTTGTTTATTATTGGAAGGACATTTACTTTCAAAACCAGACTTAACAGGA
    CAATGGGAAAAATATTTGAATGGTATTAGTAAAGGCGAAAAAGATGATGATTCATTTATA
    AATACCATTAATGAAATGATTAAAAAAACAATTAATGAAGAAGTTAAAAATAAAGAAAGT
    ATACAAAAAGTGGCCAAAGAGAAAGTATCGACTAACAATATAGCAAAATGCCCTGCTTGC
    GATAATGGCTATTTAATAGATAGAAAAGGTTTTTATGGTTGTACAGAGTACAATAACGGT
    TGTGAATTTACTATTCCTAAAAAATTGTTAGAAAAAAGTATACCTCCAACTGTAGTAAAA
    GCATTATGTGAAAGTAAGACAACAAAAAAATTGAAAGGCTTTAAATCAAAAAAAAGTGGA
    AAGTCATTTGATTGTAAGTTGATATTAACAAAAGAAAACAAACTTCAATTTTCATTCGAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2103

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_pUSA030018 [new locus tag: SAUSA300_RS14825 ]
  • symbol: TraI
  • description: transfer complex protein TraI (DNA topoisomerase III)
  • length: 700
  • theoretical pI: 8.29197
  • theoretical MW: 80742.2
  • GRAVY: -0.659

Function[edit source | edit]

  • reaction:
    EC 5.99.1.2?  ExPASy
    DNA topoisomeraseATP-independent breakage of single-stranded DNA, followed by passage and rejoining
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase III (TIGR01056; EC 5.99.1.2; HMM-score: 1090.5)
    and 4 more
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase I (TIGR01057; EC 5.99.1.2; HMM-score: 255)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase I (TIGR01051; EC 5.99.1.2; HMM-score: 201.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairreverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 106.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairrecombination protein RecR (TIGR00615; HMM-score: 19.8)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceDNA processing cluster DNA topoisomerase III (EC 5.99.1.2) 
  • PFAM:
    no clan definedTopoisom_bac; DNA topoisomerase (PF01131; HMM-score: 332.8)
    and 6 more
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 59.1)
    Zn_Beta_Ribbon (CL0167) Toprim_Crpt; C-terminal repeat of topoisomerase (PF13342; HMM-score: 58.1)
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 24.1)
    Ribos_L15p_L18e (CL0588) Ribosomal_L27A; Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A (PF00828; HMM-score: 13.5)
    Zn_Beta_Ribbon (CL0167) zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger (PF01396; HMM-score: 12.3)
    6PGD_C (CL0106) Octopine_DH; NAD/NADP octopine/nopaline dehydrogenase, alpha-helical domain (PF02317; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.34
    • Ymax_pos: 22
    • Cmax: 0.365
    • Cmax_pos: 22
    • Smax: 0.446
    • Smax_pos: 12
    • Smean: 0.3
    • D: 0.324
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNTLILCEKPSQAMDLSTVFAKKKKQNGYMEISDEQLNVSGFLTWAVGHLVELKEPQEYDEKYKNFSTYPILLEKDDFQFKVSDKTKDQFNNIKKIFKENKIDEVIIATDPAREGENIAYKILNQLKVTDKVTIKRLWLTSKVESSIRKAFKNILPKEKTYGFYKEGRARELSDWLVGINLSRHFTKISRELGNDGVIHIGRVSSPTLNMVYNRENNIKGFKGKKFYKVSATINKDEQEVKTELKNKFDSEDELHEFLFENDITDLTQKGLVTDIEKEIGYTMPPKFYDLSALQEDMNDKYKISAKRTLEIAQTLYEKKLITYPRTDSRYITEDEKEMLLENIDYLKEITKINLNNELTNNSLINPSKIEDHYAILITGNDFNKVDLKEEEINVYKSILQNVAMNFMDKEQYETTTIEIAVKKLMFEVKGKIIQDNGFKALLNKQKTSEETIPNFEKNEEVDIELDLLEKETTPPKRYTEKTLLKAMANPIETLEDEGLKSTLKEVKGLGTPATRADIIENLKKNKYIQVQKNKIYITKNGILACLLLEGHLLSKPDLTGQWEKYLNGISKGEKDDDSFINTINEMIKKTINEEVKNKESIQKVAKEKVSTNNIAKCPACDNGYLIDRKGFYGCTEYNNGCEFTIPKKLLEKSIPPTVVKALCESKTTKKLKGFKSKKSGKSFDCKLILTKENKLQFSFD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]