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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 23-MAY-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
  • pan locus tag?:
  • symbol: traK
  • pan gene symbol?:
  • synonym:
  • product: transfer complex protein TraK

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
  • symbol: traK
  • product: transfer complex protein TraK
  • replicon: pUSA03
  • strand: +
  • coordinates: 19993..21633
  • length: 1641
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACTACACTTTTAGGCGAATTAGAAAGTAAAGCAACTTCTATAAATAAAAAGAATCTA
    GTAATACAAGATTTTGATACTAAATGCCCTAATAGAAATATATTTGTGGTAGGAGGGCCA
    GGTTCCTATAAGTCAGCTGGGTATGTTATTCCTAATGTTATTGTTAAAAATCAACAATCA
    ATAGTTGTAACTGACCCTAGTGGCGAAGTTTATGAAAAAACATCTAATATTAAACGCATG
    CAAGGTTTTGATGTTAGAGTAGTAAATTTCAAAAACTTTTTAGCCTCTGATAGACAAAAC
    TTTTTTGATTATATTGATAAAGATTCTGATTGTTCAATAGTTGCTGAATTAATTATTAAA
    TCAGCTGGGGATTCTAAAATAAATAAAGACGTATGGTATAAAGCCTCTGTAGGATTATTA
    AATTCATTGCTTTTATACGCTAAATATGAATTTGAACCAGAAAAACGAACTATAGGAAAC
    ATTATTAAATTTATTCAAAATAACAAACCTAATCAAGATGAAGAAGGATCAGTTGAATTA
    GATAAACGCTTTAATGAATTATCTAAAGACCACCCAGCTAGAGAAAGTTATGAATTTGGT
    TTTGCAGTATCAGAAGGAAGAACAAGAGCAAGTATTATACTAACCTTTGTAGCCGATTTA
    CGAAATTACATTGATAACGAAATTAGAAGTTATACAAGCGATTCTGATTTTGATTTACGA
    GATGTAGGATTAAGAAAAACTATTATATATGTAATGTTGCCAGTTTTGGGGAACACTGTT
    CAATCACTTTCTAGTTTATTCTTCTCTCAAATGTTCCAACAGCTATATAGGTTAGGGGAC
    GAAAACGGTGCTAGACTTCCAGTCCCAGTAGATTTTTTACTAGACGAATGGCCAAATATC
    GGCGCAATACCAGACTATGAAGAAACTTTAGCAACTTGTCGTAAGTATGGTATTAGTATC
    ACAACAATTGTTCAATCTATATCACAAGCAGTAGATAAATATAATAAAGATAAAGCTAAC
    GCTATTATAGGTAACCACGCAGTTATTTTATGTTTAGGTAATGTGAATAATGATACAGCA
    AAATATATAAAAGAAGAATTAGGTAATGCTACTGTAGAGTTTGAAACAAGTAGTGAAGGT
    TCTTCTAGTGGTAAAGATAGCAGAAGTAAATCATCAAATAAAAATGTAAGCTACACTGGA
    AGGGCATTACTAAATGAAGATGAAATAAGTAATATGCAACAGGATAAAGCTATTCTTATA
    ACTAAAAATAGAAATCCTAAAATTATTAAAAAGTTAGCATATTTTAAAATGTTCCCTAAT
    TTAACAGAAACGTATATAGCACCTCAAAACGAATACAAACGTAAAAAAAACCAGTCTATG
    ATTGATAAGCATAATAGATTAAGAGAAGAATGGGATAAAAAACAAGAGAAAAATAAACAG
    CAAAAACTAGAAGAATATAAAGAAGAACAAAGACAGAAAGAGTTAGAGAAAGAACAACAA
    CCACAAGAAGAACAACAAAATGAGAAAGTAAAAGAAAGTAAAGACAAAAACGATCAAGAA
    GATAAGAAAGACGAGCAACAAAAAATTAATGATTCTAAGAAAGCATTCTACGAAAAATTA
    AAACAAAAACAAGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_pUSA030020 [new locus tag: SAUSA300_RS14835 ]
  • symbol: TraK
  • description: transfer complex protein TraK
  • length: 546
  • theoretical pI: 7.87684
  • theoretical MW: 62672.1
  • GRAVY: -0.789927

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Ti-type conjugative transfer system protein TraG (TIGR02767; HMM-score: 87)
    type IV conjugative transfer system coupling protein TraD (TIGR02759; HMM-score: 46.2)
    Genetic information processingMobile and extrachromosomal element functionsOtherconjugative coupling factor TraD, SXT/TOL subfamily (TIGR03743; HMM-score: 42.8)
  • TheSEED:  
    protein TrsK 
  • PFAM:
    P-loop_NTPase (CL0023) T4SS-DNA_transf; Type IV secretory system Conjugative DNA transfer (PF02534; HMM-score: 488.3)
    TraG-D_C; TraM recognition site of TraD and TraG (PF12696; HMM-score: 113.2)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 72.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 12
    • Cmax: 0.109
    • Cmax_pos: 23
    • Smax: 0.272
    • Smax_pos: 5
    • Smean: 0.217
    • D: 0.175
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEVYEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLLNSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRASIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGDENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVILCLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILITKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQRQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]