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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02690 [old locus tag: SA0467 ]
  • pan locus tag?: SAUPAN002250000
  • symbol: SA_RS02690
  • pan gene symbol?: tilS
  • synonym:
  • product: tRNA(Ile)-lysidine synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02690 [old locus tag: SA0467 ]
  • symbol: SA_RS02690
  • product: tRNA(Ile)-lysidine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 538788..540083
  • length: 1296
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (538788..540083) NCBI
  • BioCyc: G1G21-522 BioCyc
  • MicrobesOnline: see SA0467

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCAGTTAAATAGTAATGGTTGGCATGTTGATGACCATATTGTTGTCGCTGTTTCTACA
    GGTATTGATAGTATGTGTTTATTGTATCAACTACTAAATGATTATAAAGATAGTTATAGA
    AAACTAACATGCTTACATGTCAATCATGGCGTTAGGTCAGCTTCAATTGAGGAAGCCAGA
    TTTTTAGAAGCATACTGCGAACGTCATCACATTGATTTACATATCAAAAAGTTAGATTTG
    TCGCATAGTCTCGACCGAAATAACAGCATTCAGAATGAAGCTCGAATTAAACGTTACGAA
    TGGTTTGATGAAATGATGAATGTATTAGAAGCGGATGTATTGCTAACGGCGCATCATTTG
    GACGATCAATTAGAAACTATTATGTATCGTATTTTTAATGGGAAATCAACGCGTAATAAA
    CTAGGATTTGATGAGTTATCGAAGCGAAAAGGTTATCAGATTTATCGACCACTTTTAGCT
    GTCTCTAAAAAAGAAATAAAACAATTCCAAGAGAGATATCATATTCCATATTTTGAAGAT
    GAATCTAATAAAGATAACAAATATATTAGAAATGATATTCGTAATAGAATTATTCCAGCT
    ATTGATGAAAATAATCAACTTAAAGTATCGCATTTATTAAAATTAAAACAATGGCATGAT
    GAACAATATGATATTTTGCAATATTCAGCTAAACAATTTATTCAAGAATTTGTGAAGTTT
    GATGAACAGTCAAAATATTTAGAGGTTTCTAGACAAGCTTTTAATAACTTACCAAACTCA
    TTAAAGATGGTTGTGTTGGACTGCCTATTATCAAAGTATTATGAGTTGTTTAATATTAGT
    GCTAAAACATACGAAGAGTGGTTTAAACAATTTAGTAGTAAGAAAGCACAATTCAGTATT
    AATCTCACGGATAAATGGATAATTCAAATCGCATATGGTAAATTAATAATAATGGCTAAA
    AATAATGGCGATACATATTTTAGAGTTCAAACAATTAAAAAGCCAGGTAATTATTTTTTT
    AACAAATATCGATTAGAGATACATTCTAATTTACCAAAATGTTTATTTCCGCTTACAGTG
    AGAACACGACAAAGTGGCGATACATTTAAACTGAATGGGCGCGATGGTTATAAGAAAGTG
    AATCGCCTGTTTATAGATTGTAAAGTGCCACAGTGGGTTCGGGATCAAATGCCAATCGTA
    TTGGATAAACAACAGCGCATTATTGCGGTAGGAGATTTATATCAACAACAAACAATAAAA
    AAATGGATTATAATTAGTAAAAATGGAGATGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1296

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS02690 [old locus tag: SA0467 ]
  • symbol: SA_RS02690
  • description: tRNA(Ile)-lysidine synthetase
  • length: 431
  • theoretical pI: 9.35158
  • theoretical MW: 51339.5
  • GRAVY: -0.592111

Function[edit | edit source]

  • reaction:
    EC 6.3.4.19?  ExPASy
    tRNA(Ile)-lysidine synthetase (tRNA(Ile2))-cytidine34 + L-lysine + ATP = (tRNA(Ile2))-lysidine34 + AMP + diphosphate + H2O
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 196.4)
    and 3 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase, C-terminal domain (TIGR02433; EC 6.-.-.-; HMM-score: 71.6)
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 13.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 12.7)
  • TheSEED: see SA0467
  • PFAM:
    HUP (CL0039) ATP_bind_3; PP-loop family (PF01171; HMM-score: 200)
    and 3 more
    PheT-TilS (CL0383) TilS_C; TilS substrate C-terminal domain (PF11734; HMM-score: 50.5)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 22.2)
    PAPS_reduct; Phosphoadenosine phosphosulfate reductase family (PF01507; HMM-score: 20.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002522
    • TAT(Tat/SPI): 0.00021
    • LIPO(Sec/SPII): 0.00101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTCLHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWFDEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLAVSKKEIKQFQERYHIPYFEDESNKDNKYIRNDIRNRIIPAIDENNQLKVSHLLKLKQWHDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYELFNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKKPGNYFFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQWVRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

Relevant publications[edit | edit source]