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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04580 [old locus tag: SA0803 ]
  • pan locus tag?: SAUPAN003041000
  • symbol: SA_RS04580
  • pan gene symbol?: pepZ
  • synonym:
  • product: cytosol aminopeptidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS04580 [old locus tag: SA0803 ]
  • symbol: SA_RS04580
  • product: cytosol aminopeptidase
  • replicon: chromosome
  • strand: +
  • coordinates: 907312..908787
  • length: 1476
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (907312..908787) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
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    1021
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    1141
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    1261
    1321
    1381
    1441
    ATGAATTTTAAATTAAATAACACACTAAGCAACGAAATAAATACATTGATTATTGGTATA
    CCAGAACATTTAAATCAGTTAGAGCGCATTAGTTTTAATCATATCGATATTACAGAATCA
    CTTGAAAGACTAAAACATCAACATATTATTGGTAGTAAAGTTGGGAAGATTTATACAACT
    GCATTTGATGTACAAGATCAAACATATCGTTTAATTACAGTTGGTTTAGGAAACTTAAAA
    GCACGTAGTTATCAAGATATGTTGAAAATATGGGGACATCTTTTCCAATACATAAAGTCA
    GAACATATTGAAGATACTTATTTACTTATGGATTCATTTATTTCAAAATATGATCAGTTA
    TCAGATGTATTAATGGCATGCGGTATTCAAAGTGAGCGTGCAACATATGAATTCGATCAT
    TATAAATCAAGTAAGAAGGCACCGTTTAAGACGAATTTAAACCTTATTAGTGAATCATTA
    ATTGAATTAGATTTCATTCATGAGGGTATCAGTATTGGCCAATCCATTAATTTGGCAAGA
    GACTTTAGTAATATGCCACCGAATGTATTAACACCACAAACATTTGCAGAAGATATTGTT
    AATCATTTTAAAAATACAAAGGTCAAAGTAGATGTTAAAGATTATGACACTTTAGTTTCT
    GAAGGATTCGGACTTTTACAAGCAGTAGGTAAAGGTAGTAAGCATAAACCGAGATTAGTA
    ACCATCACATATAATGGCAAAGACAAAGATGTAGCACCAATTGCCTTAGTTGGTAAAGGT
    ATAACGTATGATTCTGGTGGTTATAGTATTAAAACGAAGAATGGCATGGCTACAATGAAG
    TTTGACATGTGTGGCGCTGCGAATGTCGTTGGTATCATTGAAGCGGCTAGTCGTTTACAA
    CTGCCTGTAAATATTGTCGGAGTGCTTGCTTGTGCTGAAAATATGATAAATGAAGCATCA
    ATGAAGCCAGATGATGTATTTACAGCATTAAGTGGTGAAACTGTAGAAGTAATGAATACA
    GACGCTGAAGGTAGATTAGTCCTTGCAGATGCTGTGTATTATGCAAATCAATATCAGCCT
    AGTGTGATTATGGACTTTGCTACATTAACGGGTGCAGCCATCGTTGCACTAGGCGATGAT
    AAAGCTGCTGCATTTGAATCGAATAGTAAAGTGATATTAAACGATATATTACAAATAAGT
    TCTAAAGTCGATGAAATGGTATTTGAATTACCGATTACTGCAACCGAACGTGCAAGTATT
    AATCACAGTGATATCGCTGATTTAGTTAACCATACGAATGGACAAGGTAAAGCGCTATTT
    GCGGCAAGTTTTGTAACACATTTTAGTGGTCAAACACCTCACATTCATTTCGATATTGCA
    GGTCCAGCAACGACTAATAAAGCTTCATATAATGGTCCAAAAGGGCCAACAGGATTTATG
    ATTCCGACGATAGTACAATGGTTAAAACAACAATAA
    60
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    1476

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS04580 [old locus tag: SA0803 ]
  • symbol: SA_RS04580
  • description: cytosol aminopeptidase
  • length: 491
  • theoretical pI: 5.9906
  • theoretical MW: 54069.3
  • GRAVY: -0.104481

Function[edit source | edit]

  • reaction:
    Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low?
  • TIGRFAM:
  • TheSEED:
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M17; Cytosol aminopeptidase family, catalytic domain (PF00883; HMM-score: 356.3)
    MACRO (CL0223) Peptidase_M17_N; Cytosol aminopeptidase family, N-terminal domain (PF02789; HMM-score: 62.6)
    no clan definedFliS_cochap; Flagellar FLiS export co-chaperone, HP1076 (PF16522; HMM-score: 12.6)
    TerD; TerD domain (PF02342; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.135
    • Ymax_pos: 12
    • Cmax: 0.11
    • Cmax_pos: 30
    • Smax: 0.197
    • Smax_pos: 8
    • Smean: 0.178
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446932436 NCBI
  • RefSeq: WP_001009692 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERLKHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEHIEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESLIELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTLVSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDVAPIALVGKGITYDSGGYSIKTKNGMATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGETVEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVILNDILQISSKVDEMVFELPITATERASINHSDIADLVNHTNGQGKALFAASFVTHFSGQTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS04580 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]