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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS05325 [old locus tag: SA0940 ]
  • pan locus tag?: SAUPAN003312000
  • symbol: SA_RS05325
  • pan gene symbol?: rnjA
  • synonym:
  • product: ribonuclease J 1

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS05325 [old locus tag: SA0940 ]
  • symbol: SA_RS05325
  • product: ribonuclease J 1
  • replicon: chromosome
  • strand: -
  • coordinates: 1066732..1068429
  • length: 1698
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (1066732..1068429) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAAACAATTACATCCAAATGAAGTAGGTGTATATGCACTTGGAGGTCTAGGTGAAATC
    GGTAAAAATACTTATGCAGTTGAGTATAAAGACGAAATTGTCATTATCGATGCAGGTATC
    AAATTCCCTGATGATAACTTATTAGGGATTGATTATGTTATACCTGACTACACATATCTA
    GTTCAAAACCAAGATAAAATTGTTGGCCTATTTATAACACATGGTCACGAAGACCATATA
    GGCGGTGTGCCCTTCCTATTAAAACAACTTAATATACCTATTTATGGTGGTCCTTTAGCA
    TTAGGTTTAATCCGTAATAAACTTGAAGAACATCATTTATTACGTACTGCTAAACTAAAT
    GAAATCAATGAGGACAGTGTGATTAAATCTAAGCACTTTACGATTTCTTTCTACTTAACT
    ACGCATAGTATTCCTGAAACTTATGGCGTCATCGTAGATACACCTGAAGGAAAAGTAGTT
    CATACCGGTGACTTTAAATTTGATTTTACACCTGTAGGCAAACCAGCAAACATTGCTAAA
    ATGGCTCAATTAGGCGAAGAAGGCGTTCTATGTTTACTTTCAGACTCAACAAATTCACTT
    GTGCCTGATTTTACTTTAAGCGAACGTGAAGTTGGTCAAAACGTAGATAAGATCTTCCGT
    AATTGTAAAGGTCGTATTATATTTGCTACCTTCGCTTCTAATATTTACCGAGTTCAACAA
    GCAGTTGAAGCTGCTATCAAAAATAACCGTAAAATTGTTACGTTCGGTCGTTCGATGGAA
    AACAATATTAAAATCGGTATGGAACTTGGTTATATTAAAGCACCACCTGAAACATTTATT
    GAACCTAATAAAATTAATACCGTACCGAAGCATGAGTTATTGATACTATGTACTGGTTCA
    CAAGGTGAACCAATGGCAGCATTATCTAGAATTGCTAATGGTACTCATAAGCAAATTAAA
    ATTATACCTGAAGATACCGTTGTATTTAGTTCATCACCTATCCCAGGTAATACAAAAAGT
    ATTAACAGAACTATTAATTCCTTGTATAAAGCTGGTGCAGATGTTATCCATAGCAAGATT
    TCTAACATCCATACTTCAGGGCATGGTTCTCAAGGTGATCAACAATTAATGCTTCGATTA
    ATCAAGCCGAAATATTTCTTACCTATTCATGGTGAATACCGTATGTTAAAAGCACATGGT
    GAGACTGGTGTTGAATGCGGCGTTGAAGAAGATAATGTCTTCATCTTTGATATTGGAGAT
    GTCTTAGCTTTAACACACGATTCAGCACGTAAAGCTGGTCGCATTCCATCTGGTAATGTA
    CTTGTTGATGGTAGTGGTATCGGTGATATCGGTAATGTTGTAATAAGAGACCGTAAGCTA
    TTATCTGAAGAAGGTTTAGTTATCGTTGTTGTTAGTATTGATTTTAATACAAATAAATTA
    CTTTCTGGTCCAGACATTATTTCTCGAGGATTTGTATATATGAGGGAATCAGGTCAATTA
    ATTTATGATGCACAACGCAAAATCAAAACTGATGTTATTAGTAAGTTAAATCAAAATAAA
    GATATTCAATGGCATCAGATTAAATCTTCTATCATTGAAACATTACAACCTTATTTATTT
    GAAAAAACAGCTAGAAAACCAATGATTTTACCAGTCATTATGAAGGTAAACGAACAAAAA
    GAATCAAACAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1698

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS05325 [old locus tag: SA0940 ]
  • symbol: SA_RS05325
  • description: ribonuclease J 1
  • length: 565
  • theoretical pI: 7.3306
  • theoretical MW: 62668.7
  • GRAVY: -0.169735

Function[edit source | edit]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingTranscriptionDegradation of RNAbeta-CASP ribonuclease, RNase J family (TIGR00649; EC 3.1.-.-; HMM-score: 688.9)
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 33.3)
    Genetic information processingTranscriptionRNA processingribonuclease Z (TIGR02651; EC 3.1.26.11; HMM-score: 25.1)
    Cellular processesCellular processesDetoxificationhydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 24)
    Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM-score: 12.4)
  • TheSEED:
  • PFAM:
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 62.8)
    RMMBL_DRMBL (CL0398) RMMBL; Zn-dependent metallo-hydrolase RNA specificity domain (PF07521; HMM-score: 40.6)
    Metallo-HOrase (CL0381) Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 37.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:
    SA_RS01275formate acetyltransferase  [1] (data from MRSA252)
    SA_RS017105'-nucleotidase, lipoprotein e(P4) family  [1] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [1] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [1] (data from MRSA252)
    SA_RS0292050S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA_RS0295030S ribosomal protein S7  [1] (data from MRSA252)
    SA_RS02955elongation factor G  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04420ABC transporter ATP-binding protein  [1] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [1] (data from MRSA252)
    SA_RS0631530S ribosomal protein S15  [1] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [1] (data from MRSA252)
    SA_RS0846050S ribosomal protein L20  [1] (data from MRSA252)
    SA_RS08470translation initiation factor IF-3  [1] (data from MRSA252)
    SA_RS08600universal stress protein  [1] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [1] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [1] (data from MRSA252)
    SA_RS10845DEAD/DEAH box family ATP-dependent RNA helicase  [1] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [1] (data from MRSA252)
    SA_RS1164030S ribosomal protein S11  [1] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [1] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [1] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [1] (data from MRSA252)
    SA_RS1173050S ribosomal protein L16  [1] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [1] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [1] (data from MRSA252)
    SA_RS1174530S ribosomal protein S19  [1] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [1] (data from MRSA252)
    SA_RS1176550S ribosomal protein L3  [1] (data from MRSA252)
    SA_RS13920arginine deiminase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.118
    • Ymax_pos: 27
    • Cmax: 0.155
    • Cmax_pos: 27
    • Smax: 0.131
    • Smax_pos: 1
    • Smean: 0.092
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446734119 NCBI
  • RefSeq: WP_000811375 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPDDNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALGLIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVVHTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKIFRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPPETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPIPGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGEYRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDIGNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKIKTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS05325 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]