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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06840 [old locus tag: SA1203 ]
  • pan locus tag?: SAUPAN003766000
  • symbol: SA_RS06840
  • pan gene symbol?: trpF
  • synonym:
  • product: N-(5'-phosphoribosyl)anthranilate isomerase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06840 [old locus tag: SA1203 ]
  • symbol: SA_RS06840
  • product: N-(5'-phosphoribosyl)anthranilate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 1376322..1376954
  • length: 633
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1376322..1376954) NCBI
  • BioCyc: G1G21-1382 BioCyc
  • MicrobesOnline: see SA1203

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAATTGAAATTTTGTGGCTTTACATCAATAAAGGATGTTACAGCGGCCAGTCAATTA
    CCTATTGATGCGATAGGTTTCATCCATTATGAAAAAAGTAAAAGGCATCAAACAATTACC
    CAAATAAAAAAGTTAGCGTCTGCTGTTCCAAATCATATCGATAAAGTATGTGTCATGGTA
    AATCCTGATTTAACAACAATTGAACACGTATTAAGCAATACGTCAATTAACACAATACAG
    TTACACGGCACAGAATCTATTGATTTTATACAGGAAATTAAAAAGAAATATTCAAGCATT
    AAAATCACTAAAGCTTTAGCTGCAGATGAAAACATAATCCAAAACATAAATAAATATAAA
    GGGTTCGTAGATTTATTTATTATCGACACACCCTCAGTGTCGTATGGTGGTACCGGTCAA
    ACATATGACTGGACTATTTTGAAGCACATAAAAGACATACCTTATTTGATAGCAGGAGGC
    ATTAACTCTGAAAATATTCAAACAGTTAATCAACTTAAATTATCACATCAAGGTTATGAT
    CTTGCATCAGGTATAGAAGTAAATGGGCGAAAAGATATAGAAAAAATGACAGCAATTGTA
    AATATTGTGAAAGGAGATAGAGACAATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS06840 [old locus tag: SA1203 ]
  • symbol: SA_RS06840
  • description: N-(5'-phosphoribosyl)anthranilate isomerase
  • length: 210
  • theoretical pI: 8.22155
  • theoretical MW: 23388.7
  • GRAVY: -0.201429

Function[edit | edit source]

  • reaction:
    EC 5.3.1.24?  ExPASy
    Phosphoribosylanthranilate isomerase N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
  • TIGRFAM:
    His-Xaa-Ser system putative quinone modification maturase (TIGR03981; HMM-score: 12.3)
  • TheSEED: see SA1203
  • PFAM:
    TIM_barrel (CL0036) PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 130.8)
    and 3 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 14.6)
    no clan defined Mer2; Mer2 (PF09074; HMM-score: 12.9)
    DUF1995; Domain of unknown function (DUF1995) (PF09353; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011139
    • TAT(Tat/SPI): 0.000449
    • LIPO(Sec/SPII): 0.001166
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKKLASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKITKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGGINSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: T-box(Trp), CodY see SA1203

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]