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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07650 [old locus tag: SA1350 ]
  • pan locus tag?: SAUPAN004074000
  • symbol: SA_RS07650
  • pan gene symbol?: recN
  • synonym:
  • product: DNA repair protein RecN

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS07650 [old locus tag: SA1350 ]
  • symbol: SA_RS07650
  • product: DNA repair protein RecN
  • replicon: chromosome
  • strand: -
  • coordinates: 1560370..1562049
  • length: 1680
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (1560370..1562049) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
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    481
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    1321
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    1621
    ATGTTACAAACCTTATCAATCAAGCAATTTGCTATTATTGAAGAATTAGAAATTCAATTT
    TCTGATGGTTTAACTGTTTTAAGTGGTGAAACGGGTTCAGGTAAGTCAATTATTATAGAT
    GCAATTGGACAATTAATTGGAATGAGAGCTTCATCTGATTTTGTAAGACATGGTGAAAAA
    AAGGCTGTCATCGAAGGTATATTTGATATTGACGAGAGTAAAGATGCTATACATATATTG
    AAAAATATGGATATTGATGTAGATGAAGATTTTTTATTAGTTAAACGGGAAATTTTTAGC
    TCTGGTAAAAGTCTTTGTAAAATTAATAATCAAACAGTTACTTTACAAGATTTACGAAAA
    GTTATGCAAGAATTATTAGATATTCATGGCCAACATGAAACGCAGTCTTTGTTGAAACAA
    AAATACCATTTAACATTATTGGATAATTACGCAGAATCACGTTATCAAGATTTGTTGGAT
    AAATATCACCAAACTTTTCAAAATTATAAAGCCAAAAAGCAAGAGTTAGAAGATTTAGAA
    TCAGCAGACCAAGCATTGCTGCAGCGTTTAGATTTAATGAAATTCCAATTAGAAGAATTG
    TCTGAAGCACATCTAAAAGAAGGCGAAATAGAGCAACTTGAAATAGATATTAAACGCATT
    CAAAACTCTGAAAAATTAAGCTTAGCGCTTAATAACGCACATATGACATTAACGGATGAA
    AATGCTATCACTGATCGTTTGTATGAACTAAGCAACCATTTGTTAACAATAAACGATATA
    GTTCCAAATAAATATGACAAATTAAAAGAAGATATTGATCAATTTTATTACATTTTAGAA
    GATGCAAAACATGAGTTATATGATGAAATGGCTAATACAGAATTCGACGAACAAGTGTTA
    AACGAGTATGAATCTCGTATGAATTTGCTGAATAATTTAAAACGTAAATATGGAAAAGAT
    ATTTCAGAATTAATCGCATATCAAGAAAAGCTTAATAATGAAATCAATAAAATTGAAAAC
    TATGAACAAAGTACATCGCAGTTACGAGAAGAAATAAATGCATTGTATAATCAAGTTATA
    GAGGTTGGACAAGCGTTATCAAAGCAGCGTCGTATAGTCGCTAGGGAGTTAAGAGACCAC
    ATTGTATCGGAAATTCAAAACTTACAAATGAAAGACGCAAATCTTGAGATTTCATTTAAA
    AAATTAGAAGAACCGAATATTGATGGAATCGAATTTGTAGAATTTTTAATCAGTCCAAAT
    AAAGGGGAACCATTAAAAAGTTTAAATAAAATTGCATCAGGTGGAGAACTTTCTAGAATT
    ATGTTAGCGTTAAAAAGTATTTTTGTTAAATCGAGAGGTCAAACTGCAATTTTATTCGAT
    GAGGTTGACTCAGGTGTATCTGGACAAGCCGCACAAAAAATGGCAGAGAAAATGCGAGAT
    ATAGCAGAATATATACAAGTTATTTGTATTTCTCACTTGCCACAAGTTGCATCGATGAGC
    GACCATCATTTATTAATTTCGAAGTCATCAAAAGATGATCGTACAACAACACAAGTTCAA
    GAGTTAATTGGCGATGATAAAGTAGATGAAATTGCACGAATGATTTCAGGTGCAAGTGTT
    ACTGATTTAACTCGAGAAAATGCAAGAGAAATGATACAACACAATCAAAGACGTAGATAA
    60
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    1680

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS07650 [old locus tag: SA1350 ]
  • symbol: SA_RS07650
  • description: DNA repair protein RecN
  • length: 559
  • theoretical pI: 4.73168
  • theoretical MW: 64265.4
  • GRAVY: -0.501431

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA repair protein RecN (TIGR00634; HMM-score: 545.9)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02169; HMM-score: 45.5)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02169; HMM-score: 45.5)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA replication and repair protein RecF (TIGR00611; HMM-score: 28.7)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 23.1)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 21.2)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 21.2)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 13.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesurea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 8.8)
    MetabolismTransport and binding proteinsCations and iron carrying compoundspotassium uptake protein (TIGR00794; HMM-score: 7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinMoaD family protein (TIGR01687; HMM-score: 6.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 4.1)
    thiazole/oxazole-forming peptide maturase, SagD family component (TIGR03604; HMM-score: 3.2)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 72.4)
    AAA_23; AAA domain (PF13476; HMM-score: 51.7)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 27.6)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 24.5)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 22.9)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.2)
    GH57_38_middle (CL0599) DUF1957; Domain of unknown function (DUF1957) (PF09210; HMM-score: 13.6)
    P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.1)
    AAA_22; AAA domain (PF13401; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SA_RS07730glycine dehydrogenase  [1] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [1] (data from MRSA252)
    SA_RS11010uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [1] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 24
    • Cmax: 0.126
    • Cmax_pos: 51
    • Smax: 0.139
    • Smax_pos: 18
    • Smean: 0.107
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446864954 NCBI
  • RefSeq: WP_000942210 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS07650 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]