From AureoWiki
Jump to: navigation, search

NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08655 [old locus tag: SA1538 ]
  • pan locus tag?: SAUPAN004368000
  • symbol: SA_RS08655
  • pan gene symbol?: nifZ
  • synonym:
  • product: cysteine desulfurase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS08655 [old locus tag: SA1538 ]
  • symbol: SA_RS08655
  • product: cysteine desulfurase
  • replicon: chromosome
  • strand: -
  • coordinates: 1756869..1758017
  • length: 1149
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (1756869..1758017) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGTTTATTTTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTA
    GATACTTATTTAAAAGTAAATCAATCAATGTATTATAATCCGAATAGTCCGCATAAAGCT
    GGTTTGCAGGCAAATCAATTACTACAACAAGCAAAAGCCCAAATTAATGCAATGATTAAT
    TCAAAAACAAATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCT
    TTAAAAGGTATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTA
    GAGCATCCGTCCGTATTAGAGGTTGTAAGATATTTGGAAGCACACGAAGGATTTAAAGTT
    AAATATGTTGATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCAAAGAATTAGTG
    TCAGACAAAGTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAG
    CCTATTCCACAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGAT
    GCGGTTCAAGCATTCGGCAAAATTTCAATGGATCTCAATAACATAGATAGTATTAGTTTA
    AGTGGACACAAGTTTAATGGTTTAAAAGGACAAGGCGTCTTACTTGTAAATCACATTCAA
    AATGTTGAACCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTT
    AATTTGCCAAATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAA
    GCATTGAATGCATTTGTTACTGAGTTAAATAATGACGTCCGTCAATTTTTAAATAAATAT
    CATGGAGTTTATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAAATATTAGTTTT
    CCTGGCGTAAAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCT
    ACGACGAGTGCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGA
    TTATCAGACAAATCTATTGAAGGTAGTATAAGATTATCATTTGGGGCTACTACAACTAAA
    GAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAATTAAGGAGTTG
    CTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1149

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS08655 [old locus tag: SA1538 ]
  • symbol: SA_RS08655
  • description: cysteine desulfurase
  • length: 382
  • theoretical pI: 8.1916
  • theoretical MW: 42303.2
  • GRAVY: -0.113613

Function[edit source | edit]

  • TIGRFAM:
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 310.8)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 298.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationcysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 282.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 282.1)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 250.3)
    and 8 more
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 139.1)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 132.5)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01976; HMM-score: 86.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 76.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 44.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthertyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 29.3)
    MetabolismCentral intermediary metabolismPhosphorus compounds2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 24.9)
    putative modified peptide (TIGR04509; HMM-score: 12.4)
  • TheSEED:
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 235.6)
    and 3 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 23.1)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 16)
    EF_hand (CL0220) EF-hand_1; EF hand (PF00036; HMM-score: 13.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.168
    • Ymax_pos: 16
    • Cmax: 0.121
    • Cmax_pos: 54
    • Smax: 0.383
    • Smax_pos: 10
    • Smean: 0.227
    • D: 0.191
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_012840521 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS08655 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]