NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08850 [old locus tag: SA1569 ]
• pan locus tag^?: SAUPAN004425000
• symbol: SA_RS08850
• pan gene symbol^?: trmB
• synonym:
• product: tRNA (guanosine(46)-N7)-methyltransferase TrmB

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08850 [old locus tag: SA1569 ]
• symbol: SA_RS08850
• product: tRNA (guanosine(46)-N7)-methyltransferase TrmB
• replicon: chromosome
• strand: -
• coordinates: 1799557..1800201
• length: 645
• essential: unknown

⊟Accession numbers

• Location: NC_002745 (1799557..1800201) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAGAGTTCGATACAAACCATGGGCTGAAGATTATTTAAAAGATCATCCTGAATTGGTT
  GACATGGAAGGTCAACATGCAGGGGAAATGACTGAATGGTTTGATAAAACGCAACCGATA
  CACATTGAAATTGGTTCGGGTATGGGGCAGTTTATTACAACATTAGCTGCTCAAAATCCT
  CATATTAACTATATTTCAATGGAACGTGAGAAAAGTATCGTTTATAAAGTATTGGATAAA
  GTTAAAGAGATGGGTTTAACAAATTTAAAGATTATTTGTAATGACGCCATTGAATTAAAT
  GAATATTTTAAAGACGGCGAAGTTTCGCGTATTTATTTGAACTTTTCAGATCCATGGCCA
  AAAAATCGTCATGCAAAACGACGTTTAACGTATCATACGTTTTTAGCGTTGTATCAACAA
  ATCTTAAATGATGAAGGAGATTTACATTTTAAAACTGATAACAGAGGACTATTTGCCTAC
  AGTTTAGAAAGTATGTCTCAATTTGGAATGTATTTTACAAAAATAAATCTAAATTTACAT
  CAAGAAGACGATGGCAGTAATATTTTAACAGAATATGAAAAGAAATTTTCGGATAAAGGG
  TCACGTATTTATCGTATGGAAGCGAAATTCCATTCACAAAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS08850 [old locus tag: SA1569 ]
• symbol: SA_RS08850
• description: tRNA (guanosine(46)-N7)-methyltransferase TrmB
• length: 214
• theoretical pI: 6.60791
• theoretical MW: 25290.5
• GRAVY: -0.706075

⊟Function

• reaction:
  EC 2.1.1.33?  ExPASy
  tRNA (guanine⁴⁶-N⁷)-methyltransferaseS-adenosyl-L-methionine + guanine⁴⁶ in tRNA = S-adenosyl-L-homocysteine + N⁷-methylguanine⁴⁶ in tRNA
• TIGRFAM:
  Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 184.1)
  Genetic information processingProtein synthesistRNA and rRNA base modificationribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 15.9)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinmalonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.7)
  Genetic information processingProtein fateProtein modification and repairprotein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 13.2)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.2)
• TheSEED:
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 169.6)
  DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 17.9)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 15.2)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 15)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.3)
  Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 12.2)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 12.1)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.099
  • Ymax_pos: 69
  • Cmax: 0.118
  • Cmax_pos: 57
  • Smax: 0.139
  • Smax_pos: 56
  • Smean: 0.072
  • D: 0.089
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447188905 NCBI
• RefSeq: WP_001266161 NCBI
• UniProt:

⊟Protein sequence

• MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SA_RS08850 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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