NCBI: 02-MAR-2017
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS08850 [old locus tag: SA1569 ]
- pan locus tag?: SAUPAN004425000
- symbol: SA_RS08850
- pan gene symbol?: trmB
- synonym:
- product: tRNA (guanosine(46)-N7)-methyltransferase TrmB
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SA_RS08850 [old locus tag: SA1569 ]
- symbol: SA_RS08850
- product: tRNA (guanosine(46)-N7)-methyltransferase TrmB
- replicon: chromosome
- strand: -
- coordinates: 1799557..1800201
- length: 645
- essential: unknown
⊟Accession numbers[edit source | edit]
- Location: NC_002745 (1799557..1800201) NCBI
- MicrobesOnline:
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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601ATGAGAGTTCGATACAAACCATGGGCTGAAGATTATTTAAAAGATCATCCTGAATTGGTT
GACATGGAAGGTCAACATGCAGGGGAAATGACTGAATGGTTTGATAAAACGCAACCGATA
CACATTGAAATTGGTTCGGGTATGGGGCAGTTTATTACAACATTAGCTGCTCAAAATCCT
CATATTAACTATATTTCAATGGAACGTGAGAAAAGTATCGTTTATAAAGTATTGGATAAA
GTTAAAGAGATGGGTTTAACAAATTTAAAGATTATTTGTAATGACGCCATTGAATTAAAT
GAATATTTTAAAGACGGCGAAGTTTCGCGTATTTATTTGAACTTTTCAGATCCATGGCCA
AAAAATCGTCATGCAAAACGACGTTTAACGTATCATACGTTTTTAGCGTTGTATCAACAA
ATCTTAAATGATGAAGGAGATTTACATTTTAAAACTGATAACAGAGGACTATTTGCCTAC
AGTTTAGAAAGTATGTCTCAATTTGGAATGTATTTTACAAAAATAAATCTAAATTTACAT
CAAGAAGACGATGGCAGTAATATTTTAACAGAATATGAAAAGAAATTTTCGGATAAAGGG
TCACGTATTTATCGTATGGAAGCGAAATTCCATTCACAAAAATAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SA_RS08850 [old locus tag: SA1569 ]
- symbol: SA_RS08850
- description: tRNA (guanosine(46)-N7)-methyltransferase TrmB
- length: 214
- theoretical pI: 6.60791
- theoretical MW: 25290.5
- GRAVY: -0.706075
⊟Function[edit source | edit]
- reaction: EC 2.1.1.33? ExPASytRNA (guanine46-N7)-methyltransferaseS-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
- TIGRFAM: Protein synthesistRNA and rRNA base modificationtRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 184.1)Protein synthesistRNA and rRNA base modificationribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 15.9)Biosynthesis of cofactors, prosthetic groups, and carriersBiotinmalonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.7)Protein fateProtein modification and repairprotein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 13.2)Biosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.2)
- TheSEED:
- PFAM: NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 169.6)DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 17.9)Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 15.2)PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 15)Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.3)Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 12.2)GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 12.1)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- Ymax: 0.099
- Ymax_pos: 69
- Cmax: 0.118
- Cmax_pos: 57
- Smax: 0.139
- Smax_pos: 56
- Smean: 0.072
- D: 0.089
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MRVRYKPWAEDYLKDHPELVDMEGQHAGEMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- SA_RS08850 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]