NCBI: 02-MAR-2017
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus N315
- locus tag: SA_RS09180 [old locus tag: SA1631 ]
- pan locus tag?: SAUPAN004567000
- symbol: SA_RS09180
- pan gene symbol?: splA
- synonym:
- product: serine protease SplA
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SA_RS09180 [old locus tag: SA1631 ]
- symbol: SA_RS09180
- product: serine protease SplA
- replicon: chromosome
- strand: -
- coordinates: 1864410..1865117
- length: 708
- essential: unknown
⊟Accession numbers[edit source | edit]
- Location: NC_002745 (1864410..1865117) NCBI
- MicrobesOnline:
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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661ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACATCTCTTGGT
TTCGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAGAATGTCAAA
GAAATTACCGATGCAACTAAGGCACCATACAATTCAGTGGTAGCATTTGCGGGTGGTACT
GGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAAAGTAATGAT
ATTTTTAAAAATAGAGTAGCAGCACATTATTCGAGTAAAGGTAAAGGCGGAGGAAACTAC
GACGTTAAAGACATTGTAGAATATCCTGGAAAAGAAGACCTTGCGATAGTTCATGTTCAT
GAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAATTTGCAGAA
GGAGCAAAAGCGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCACAAACAAAA
TATAAAATGTTTGAATCGACAGGAACGATTAACCATATCAGTGGAACGTTTATAGAATTT
GATGCGTATGCACAACCAGGTAATTCAGGATCTCCTGTATTGAATTCTAAACATGAACTG
ATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTCGGTGTTTAT
TTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SA_RS09180 [old locus tag: SA1631 ]
- symbol: SA_RS09180
- description: serine protease SplA
- length: 235
- theoretical pI: 9.61883
- theoretical MW: 25440.5
- GRAVY: -0.398723
⊟Function[edit source | edit]
- reaction: EC 3.4.21.-? ExPASy
- TIGRFAM: Protein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 28.4)Regulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 28.4)Protein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 25.2)Protein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 25.2)
- TheSEED:
- PFAM: Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 72.1)Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 59.2)Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.6)Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.6)Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 18.6)Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 13.5)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.73
- Internal Helices: 0
- LocateP:
- SignalP: Signal peptide length 36 aa
- Ymax: 0.424
- Ymax_pos: 36
- Cmax: 0.391
- Cmax_pos: 36
- Smax: 0.846
- Smax_pos: 2
- Smean: 0.645
- D: 0.51
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITDATKAPYNSVVAFAGGTGVVVGKNTIVTNKHIAKSNDIFKNRVAAHYSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFAEGAKAKDRISVIGYPKGAQTKYKMFESTGTINHISGTFIEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- SA_RS09180 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]