NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• pan locus tag^?: SAUPAN005303000
• symbol: SA_RS10670
• pan gene symbol^?: ilvD
• synonym:
• product: dihydroxy-acid dehydratase

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• symbol: SA_RS10670
• product: dihydroxy-acid dehydratase
• replicon: chromosome
• strand: +
• coordinates: 2097592..2099280
• length: 1689
• essential: unknown

⊟Accession numbers

• Location: NC_002745 (2097592..2099280) NCBI
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  
  ATGCGAAGCGACATGATCAAAAAAGGAGATCACCAAGCACCAGCAAGAAGTCTTTTACAT
  GCCACGGGCGCGCTAAAAAGTCCAACTGATATGAACAAACCATTTGTAGCTATTTGTAAC
  TCTTATATTGATATTGTTCCTGGACATGTTCACTTGAGAGAGCTTGCAGATATAGCTAAA
  GAAGCAATTAGAGAAGCCGGTGCCATTCCATTTGAATTCAATACAATTGGTGTTGATGAT
  GGAATAGCTATGGGACATATCGGAATGCGATATTCTCTACCATCACGTGAAATTATTGCA
  GATGCAGCTGAAACTGTAATTAACGCTCATTGGTTTGACGGCGTATTTTACATTCCTAAT
  TGTGACAAGATTACACCCGGTATGATTTTAGCAGCCATGAGGACAAACGTACCAGCTATC
  TTTTGCTCTGGTGGACCAATGAAAGCTGGCTTATCTGCACATGGAAAAGCATTAACACTT
  TCATCAATGTTTGAAGCAGTCGGCGCATTTAAAGAAGGATCGATTTCTAAAGAAGAATTT
  TTAGATATGGAACAAAATGCCTGCCCTACTTGTGGTTCATGTGCTGGGATGTTTACTGCA
  AATTCAATGAACTGTTTGATGGAAGTTTTAGGTCTAGCATTACCATACAACGGTACTGCA
  CTTGCAGTCAGTGATCAGCGACGCGAAATGATTCGCCAAGCAGCTTTTAAATTAGTTGAA
  AATATTAAAAATGATTTAAAACCACGTGATATCGTTACTCGCGAAGCAATTGATGATGCA
  TTTGCACTTGATATGGCTATGGGTGGTTCAACAAACACGGTACTGCATACGTTAGCCATT
  GCCAATGAAGCTGGTATTGATTATGACTTAGAGCGCATTAATGCTATTGCCAAACGCACG
  CCATATTTATCAAAAATAGCACCTAGTTCATCGTATTCAATGCATGATGTGCATGAAGCT
  GGTGGCGTCCCAGCAATTATTAATGAATTGATGAAGAAAGATGGCACGTTACACCCAGAT
  AGAATCACAGTTACTGGCAAAACGTTACGTGAAAATAACGAAGGCAAAGAAATTAAGAAC
  TTTGATGTCATTCACCCTCTTGATGCACCATATGATGCACAAGGCGGTTTATCTATCTTA
  TTTGGTAATATCGCCCCTAAAGGCGCAGTTATTAAAGTTGGCGGCGTTGATCCATCTATC
  AAAACATTTACTGGGAAAGCAATTTGTTTCAATTCGCATGATGAAGCTGTTGAAGCAATA
  GACAATCGTACCGTTCGTGCAGGCCACGTCGTTGTCATTAGATATGAAGGACCTAAAGGT
  GGACCAGGTATGCCTGAAATGTTAGCACCTACTTCCTCTATTGTTGGTCGCGGCTTAGGT
  AAAGATGTTGCATTAATTACTGATGGGCGTTTTTCCGGTGCCACAAGAGGTATTGCAGTT
  GGTCATATTTCCCCTGAAGCTGCATCTGGTGGACCAATTGCCTTAATTGAAGATGGAGAT
  GAGATTACTATTGATTTAACAAATCGTACATTAAACGTAAACCAGCCTGAAGATGTTCTA
  GCGCGTCGCCGAGAATCTTTAACACCGTTTAAAGCGAAAGTAAAAACAGGTTATCTAGCT
  CGTTATACTGCCCTAGTAACTAGCGCAAATACAGGTGGCGTCATGCAAGTCCCTGAGAAT
  TTAATTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1689
  

⊟Protein

⊟General

• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• symbol: SA_RS10670
• description: dihydroxy-acid dehydratase
• length: 562
• theoretical pI: 6.24187
• theoretical MW: 60021.5
• GRAVY: -0.0540925

⊟Function

• reaction:
  EC 4.2.1.9?  ExPASy
  Dihydroxy-acid dehydratase2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H₂O
• TIGRFAM:
  MetabolismAmino acid biosynthesisPyruvate familydihydroxy-acid dehydratase (TIGR00110; EC 4.2.1.9; HMM-score: 784.1)
  MetabolismEnergy metabolismEntner-Doudoroffphosphogluconate dehydratase (TIGR01196; EC 4.2.1.12; HMM-score: 285.5)
  Unknown functionEnzymes of unknown specificityputative dehydratase, YjhG/YagF family (TIGR03432; HMM-score: 223.1)
• TheSEED:
• PFAM:
  no clan definedILVD_EDD; Dehydratase family (PF00920; HMM-score: 736.2)
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 12.5)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors: [4Fe-4S] cluster
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.159
  • Ymax_pos: 20
  • Cmax: 0.142
  • Cmax_pos: 53
  • Smax: 0.316
  • Smax_pos: 14
  • Smean: 0.239
  • D: 0.19
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447178524 NCBI
• RefSeq: WP_001255780 NCBI
• UniProt:

⊟Protein sequence

• MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYIDIVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADAAETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTLSSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNGTALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLHTLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKDGTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIKVGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTNRTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SA_RS10670 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.