NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• pan locus tag^?: SAUPAN005303000
• symbol: SA_RS10670
• pan gene symbol^?: ilvD
• synonym:
• product: dihydroxy-acid dehydratase

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• symbol: SA_RS10670
• product: dihydroxy-acid dehydratase
• replicon: chromosome
• strand: +
• coordinates: 2097592..2099280
• length: 1689
• essential: unknown

⊟Accession numbers

• Location: NC_002745 (2097592..2099280) NCBI
• BioCyc: G1G21-2156 BioCyc
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  
  ATGCGAAGCGACATGATCAAAAAAGGAGATCACCAAGCACCAGCAAGAAGTCTTTTACAT
  GCCACGGGCGCGCTAAAAAGTCCAACTGATATGAACAAACCATTTGTAGCTATTTGTAAC
  TCTTATATTGATATTGTTCCTGGACATGTTCACTTGAGAGAGCTTGCAGATATAGCTAAA
  GAAGCAATTAGAGAAGCCGGTGCCATTCCATTTGAATTCAATACAATTGGTGTTGATGAT
  GGAATAGCTATGGGACATATCGGAATGCGATATTCTCTACCATCACGTGAAATTATTGCA
  GATGCAGCTGAAACTGTAATTAACGCTCATTGGTTTGACGGCGTATTTTACATTCCTAAT
  TGTGACAAGATTACACCCGGTATGATTTTAGCAGCCATGAGGACAAACGTACCAGCTATC
  TTTTGCTCTGGTGGACCAATGAAAGCTGGCTTATCTGCACATGGAAAAGCATTAACACTT
  TCATCAATGTTTGAAGCAGTCGGCGCATTTAAAGAAGGATCGATTTCTAAAGAAGAATTT
  TTAGATATGGAACAAAATGCCTGCCCTACTTGTGGTTCATGTGCTGGGATGTTTACTGCA
  AATTCAATGAACTGTTTGATGGAAGTTTTAGGTCTAGCATTACCATACAACGGTACTGCA
  CTTGCAGTCAGTGATCAGCGACGCGAAATGATTCGCCAAGCAGCTTTTAAATTAGTTGAA
  AATATTAAAAATGATTTAAAACCACGTGATATCGTTACTCGCGAAGCAATTGATGATGCA
  TTTGCACTTGATATGGCTATGGGTGGTTCAACAAACACGGTACTGCATACGTTAGCCATT
  GCCAATGAAGCTGGTATTGATTATGACTTAGAGCGCATTAATGCTATTGCCAAACGCACG
  CCATATTTATCAAAAATAGCACCTAGTTCATCGTATTCAATGCATGATGTGCATGAAGCT
  GGTGGCGTCCCAGCAATTATTAATGAATTGATGAAGAAAGATGGCACGTTACACCCAGAT
  AGAATCACAGTTACTGGCAAAACGTTACGTGAAAATAACGAAGGCAAAGAAATTAAGAAC
  TTTGATGTCATTCACCCTCTTGATGCACCATATGATGCACAAGGCGGTTTATCTATCTTA
  TTTGGTAATATCGCCCCTAAAGGCGCAGTTATTAAAGTTGGCGGCGTTGATCCATCTATC
  AAAACATTTACTGGGAAAGCAATTTGTTTCAATTCGCATGATGAAGCTGTTGAAGCAATA
  GACAATCGTACCGTTCGTGCAGGCCACGTCGTTGTCATTAGATATGAAGGACCTAAAGGT
  GGACCAGGTATGCCTGAAATGTTAGCACCTACTTCCTCTATTGTTGGTCGCGGCTTAGGT
  AAAGATGTTGCATTAATTACTGATGGGCGTTTTTCCGGTGCCACAAGAGGTATTGCAGTT
  GGTCATATTTCCCCTGAAGCTGCATCTGGTGGACCAATTGCCTTAATTGAAGATGGAGAT
  GAGATTACTATTGATTTAACAAATCGTACATTAAACGTAAACCAGCCTGAAGATGTTCTA
  GCGCGTCGCCGAGAATCTTTAACACCGTTTAAAGCGAAAGTAAAAACAGGTTATCTAGCT
  CGTTATACTGCCCTAGTAACTAGCGCAAATACAGGTGGCGTCATGCAAGTCCCTGAGAAT
  TTAATTTAA
  60
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  240
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  360
  420
  480
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  840
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  1020
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  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1689
  

⊟Protein

⊟General

• locus tag: SA_RS10670 [old locus tag: SA1858 ]
• symbol: SA_RS10670
• description: dihydroxy-acid dehydratase
• length: 562
• theoretical pI: 6.24187
• theoretical MW: 60021.5
• GRAVY: -0.0540925

⊟Function

• reaction:
  EC 4.2.1.9?  ExPASy
  Dihydroxy-acid dehydratase2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H₂O
• TIGRFAM:
  MetabolismAmino acid biosynthesisPyruvate familydihydroxy-acid dehydratase (TIGR00110; EC 4.2.1.9; HMM-score: 784.1)
  and 2 more
  MetabolismEnergy metabolismEntner-Doudoroffphosphogluconate dehydratase (TIGR01196; EC 4.2.1.12; HMM-score: 285.5)
  Unknown functionEnzymes of unknown specificityputative dehydratase, YjhG/YagF family (TIGR03432; HMM-score: 223.1)
• TheSEED:
• PFAM:
  no clan definedILVD_EDD; Dehydratase family (PF00920; HMM-score: 736.2)
  and 1 more
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 12.5)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors: [4Fe-4S] cluster
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.159
  • Ymax_pos: 20
  • Cmax: 0.142
  • Cmax_pos: 53
  • Smax: 0.316
  • Smax_pos: 14
  • Smean: 0.239
  • D: 0.19
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 447178524 NCBI
• RefSeq: WP_001255780 NCBI
• UniProt:

⊟Protein sequence

• MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYIDIVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADAAETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTLSSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNGTALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLHTLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKDGTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIKVGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAPTSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTNRTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SA_RS10670 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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