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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS11890 [old locus tag: SA2067 ]
  • pan locus tag?: SAUPAN005733000
  • symbol: SA_RS11890
  • pan gene symbol?: mobB
  • synonym:
  • product: molybdopterin-guanine dinucleotide biosynthesis protein B

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS11890 [old locus tag: SA2067 ]
  • symbol: SA_RS11890
  • product: molybdopterin-guanine dinucleotide biosynthesis protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 2328999..2329484
  • length: 486
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2328999..2329484) NCBI
  • BioCyc: G1G21-2410 BioCyc
  • MicrobesOnline: see SA2067

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGATTTTACAAATTGTAGGTTACAAAAAGTCTGGTAAGACAACATTGATGAGGCATATT
    GTCTCTTTCTTAAAGACACATGGTTATACAGTTGCTACTATTAAACATCATGGGCATGCT
    AAGGAAGATATTCAATTACAGGATTCAGACGTCGATCACATGAAGCATTTTGAAGCGGGG
    GCAGATCAAAGTATTGTACAAGGTTTTCAATATCAGCAAACTGTAACACGTGTAGATAAT
    CAAAATCTTACTCAAATTATTGAGAAATCTGTTACAATTGACACCAATATCGTATTAGTT
    GAAGGCTTTAAAAATGCTGATTTTGAAAAAGTCGTAGTCTATCGAGATGAAGAAGAGTTG
    CAAGTATTACAACAATTGTCGAATGTTTGTTATAGCATTAATGTAAGGGAGCATGAAGAT
    TTTACAGCATTTGAGCAATGGTTATTAAATAAAATTAAAAATGATTGTGATACACAATTA
    ACATAG
    60
    120
    180
    240
    300
    360
    420
    480
    486

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS11890 [old locus tag: SA2067 ]
  • symbol: SA_RS11890
  • description: molybdopterin-guanine dinucleotide biosynthesis protein B
  • length: 161
  • theoretical pI: 5.80994
  • theoretical MW: 18597.9
  • GRAVY: -0.424845

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 131.7)
    and 8 more
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 17.4)
    DNA repair and recombination protein RadB (TIGR02237; HMM-score: 16.3)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 14.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine kinase (TIGR00235; EC 2.7.1.48; HMM-score: 14)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.7)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 12.4)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein B (TIGR02900; HMM-score: 11.4)
  • TheSEED: see SA2067
  • PFAM:
    P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 97.8)
    and 10 more
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 21.2)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 18.5)
    AAA_30; AAA domain (PF13604; HMM-score: 17.7)
    AAA_14; AAA domain (PF13173; HMM-score: 14.8)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.5)
    AAA_18; AAA domain (PF13238; HMM-score: 14)
    DUF2478; Protein of unknown function (DUF2478) (PF10649; HMM-score: 12.7)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.7)
    AAA_22; AAA domain (PF13401; HMM-score: 12.4)
    CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.044155
    • TAT(Tat/SPI): 0.001374
    • LIPO(Sec/SPII): 0.002381
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MILQIVGYKKSGKTTLMRHIVSFLKTHGYTVATIKHHGHAKEDIQLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEGFKNADFEKVVVYRDEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQLT

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]