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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0302 [new locus tag: NWMN_RS01720 ]
  • pan locus tag?: SAUPAN000618000
  • symbol: NWMN_0302
  • pan gene symbol?:
  • synonym:
  • product: phage tape measure protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0302 [new locus tag: NWMN_RS01720 ]
  • symbol: NWMN_0302
  • product: phage tape measure protein
  • replicon: chromosome
  • strand: +
  • coordinates: 347122..350265
  • length: 3144
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    TTGGATACTCATTTTATGGCAAAGATTATGGCCAATATTAGAGATTTCCAAAGCAACGTA
    AGGAAAGCTCAACGATTAGCAAAGACGTCTGTACCAAACGAAATTGAAACAGATGTAAAA
    GCAGATATTTCAAGATTCCAAAGAGCTTTACAACGCGCTAAATCAATGGCTCAACGATGG
    CGAGAGCATTCTGTTAAATTATTCATGAAAACAGATGAGTATAAAGCGAATTTAGAACGC
    GCTAAAGCTCAAGTAGAGCGATTTAAACAACATAAAGTAGATTTGAAACTAAGTAACACT
    GAATTAATGGCCAAATATAATGCAACTAAAGCTACTGTCGAAGCTTGGAGAAAACATGTT
    GTTAAGTTGGATTTAGATGCAAACCCCGCTAAAATGGCGGTTAAAGGGTTTAAAGAAGAT
    TTAATAGATCTTAGCAGGCATAGTTTTGATATTGATTCCAGCAGATGGAAATTAGGAAAT
    AAATTCACAAAAGAATTCAATGAAGTCGAAGGAGCAGTTAAACGTTCTTTCGGAAGAATT
    GGTCAGATTATGAGAAAAGAAGTAAATGGAACAAGTGATATTTGGGGTAAACTTAACAAC
    TCATTGAAAGATTACGGCGAGAAAATGGACGCCTTAGCTACTAAAATCCGAACTTTCGGT
    ACTATCTTCGCGCAACAGGTCAAAGGCTTAATGATTGCTAGTATACAAGCATTGATACCA
    GTGATTGCCGGATTAGTACCTGCAATAATGGCAGTACTTAATGCGGTTGGTGTATTAGGT
    GGTGGCGTTTTAGGTTTAGTTGGCGCATTCTCTGTCGCAGGTCTTGGAGTTGTTGGCTTT
    GGTGCAATGGCTATTAGCGCTCTTAAAATGGTTGAAGATGGAACATTGGCAGTAACAAAA
    GAAGTTCAAAACTTTAGAGATGCGAGCGATCAGTTAAAAACTACATGGCGTGATATTGTT
    AAAGAGAATCAAGCAAGTATCTTTAATGCGATGTCAGCAGGTATCAGAGGTGTTACAAGT
    GCGATGTCTCAATTAAAACCATTCTTATCTGAAGTATCTATGCTGGTTGAAGCAAACGCA
    CGCAAGTTTGAGGATTGGGTTAAACATTCTGAAACAACTAAGAAAGCATTTGAAGCATTG
    AATAGCATAGGTGGCGCAATCTTCGGAGATTTATTGAACGCTGCAGGAAGATTTGGCGAC
    GGATTAATTAACATTTTCACTCAATTAATGCCGTTGTTCAAATTTGTGTCTCAAGGACTA
    CAGAACATGTCCATAGCTTTCCAAAATTGGGCTAATAGTGTGGCTGGTCAGAATGCTATT
    AAAGCGTTTATTGACTACACTACCACTAACTTACCTAAGATTGGTCAGATATTTGGCAAT
    GTGTTCGCTGGTATTGGTAATTTAATGATTGCTTTTGCTCAAAACAGTTCTAACATTTTT
    GACTGGTTAGTTAAATTAACTTCTCAATTTAGAGCATGGTCAGAACAAGTAGGACAATCA
    CAAGGATTTAAAGACTTTATCAGTTACGTTCAAGAGAATGGTCCTACTATTATGCAGTTA
    ATCGGTAATATCGTAAAAGCGTTAGTGGCATTTGGTACTGCAATGGCTCCTATAGCTAGT
    AAATTACTAGATTTCGTTACTAATTTAGCTGGATTTATCGCCAAACTATTCGAAGCACAC
    CCAGCAGTCGCTCAAATTATCGGTGTTATCGGTATTTTAGGTGGCGTATTTTGGGCTTTA
    ATGGCTCCGATTGCAGCTGTTAGCAGTGTGTTAAGTAATGTGTTTAGTATGACTTTATTG
    AATGTTGTCAAAAGAATACTGGATTTAACTAGAATAACTGGGGTGGTAAGTAAAGCGTTC
    GGTTTATTGACTGGTGCTTTCACAAGTATTTCTTGGCCAATATTAGCAGTAGTTGCAGTC
    ATTGGTGTATTCATTGGTATTCTTGTTTATTTATGGAAAACAAACGAGAATTTCAGAAAA
    ACAATAACAGAAGCTTGGAACGGTATTAAAACAGCAGTTTCCGGTGCGATTCAAGGTGTA
    GTAGATTGGTTAACTCAATTGTGGGGCAAAATTCAATCAACATTACAGTCAATCATGCCT
    ATATTACAAGTATTAGGACAAGTATTCATGCAAGTTTTAGGTGTTTTGGTAATAGGCATT
    ATTACAAATGTTATGAATATCATACAAGGTTTGTGGACGTTAATTACAATTGCGTTCCAA
    GCCATAGGAACAGTGATATCCGTAGCTGTCCAAATCATAGTAGGTTTATTCACTGCTTTA
    ATTCAGTTGCTTACTGGCGACTTCTCAGGTGCTTGGGAGACTATTAAAACTACGGTTACC
    AATGTACTTGATACGATTTGGCAATACATGCAATCAGTTTGGGAGTCAATTATCGGCTTT
    TTAACTGGCGTAATGAATCGAACGCTTTCAATGTTTGGTACAAGTTGGTCACAGATATGG
    AGTACAATCACTAATTTTGTTAGCAGTATTTGGAGCACTGTTACAAGTTGGTTCAGTCGT
    GTTGCTTGGAGTGTGGCTGAAAAAATGGGACAAGCATTAAACTTTATTATCACAAAAGGT
    TCTGAATGGGTTTCTAACATTTGGAATACAGTTACAAGTTTCGCGAGTAAAGTAGCTGAT
    GGGTTTAAAAGAGTTGTCTCAAATGTAGGTGACGGTATGAGTGATGCACTTGGTAAGATT
    AAAAGTTTCTTCAGTGATTTCTTAAATGCCGGAGCGGAATTAATCGGCAAAGTAGCTGAG
    GGTGTAGCCAATGCTGCGCACAAAGTAGTCAGCGCGGTAGGCGATGCGATTTCATCAGCT
    TGGGACTCTGTAACTTCATTCGTAAGTGGACACGGTGGAGGTAGTAGCTTAGGTAAAGGT
    TTAGCGGTATCACAAGCAAAAGTAATTGCTACAGACTTTGGCAGTGCCTTTAATAAAGAG
    CTATCCTCTACTTTGACAGATAGTATAGTAAATCCTGTAAGTACTTCTATAGACAGACAC
    ATGACTAGCGATGTTCAACATAGCTTAAAAGAAAATAATAGACCTATTGTGAATGTAACG
    ATTAGAAATGAGGGCGACCTTGATTTAATTAAATCACGCATTGATGACATGAACGCTATA
    GACGGAAGTTTCAACTTATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3144

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0302 [new locus tag: NWMN_RS01720 ]
  • symbol: NWMN_0302
  • description: phage tape measure protein
  • length: 1047
  • theoretical pI: 10.1899
  • theoretical MW: 113906
  • GRAVY: 0.253868

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 19.1)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 19.1)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 17.3)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 17.3)
    and 1 more
    ABC exporter membrane fusion protein, DevB family (TIGR02971; HMM-score: 9.4)
  • TheSEED:
  • PFAM:
    no clan defined Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 30.4)
    and 16 more
    Vps51 (CL0295) Sec8_exocyst; Sec8 exocyst complex component specific domain (PF04048; HMM-score: 17.1)
    no clan defined Chlorosome_CsmC; Chlorosome envelope protein C (PF11098; HMM-score: 16.2)
    DUF4201; Domain of unknown function (DUF4201) (PF13870; HMM-score: 13.3)
    Prominin; Prominin (PF05478; HMM-score: 13.2)
    Ins_allergen_rp; Insect allergen related repeat, nitrile-specifier detoxification (PF06757; HMM-score: 13.2)
    Senescence; Senescence-associated protein (PF06911; HMM-score: 13.2)
    HAUS6_N; HAUS augmin-like complex subunit 6 N-terminus (PF14661; HMM-score: 12.8)
    DUF2884; Protein of unknown function (DUF2884) (PF11101; HMM-score: 12.2)
    P-loop_NTPase (CL0023) DUF3584; Protein of unknown function (DUF3584) (PF12128; HMM-score: 12.2)
    no clan defined PEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 11.1)
    TMP; TMP repeat (PF05017; HMM-score: 11)
    DUF4363; Domain of unknown function (DUF4363) (PF14276; HMM-score: 11)
    Tape_meas_lam_C; Lambda phage tail tape-measure protein (Tape_meas_lam_C) (PF09718; HMM-score: 10.4)
    Picorna_P3A; Picornaviridae P3A protein (PF06363; HMM-score: 9.1)
    Not3; Not1 N-terminal domain, CCR4-Not complex component (PF04065; HMM-score: 8.9)
    IL32; Interleukin 32 (PF15225; HMM-score: 8.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004385
    • TAT(Tat/SPI): 0.001163
    • LIPO(Sec/SPII): 0.001128
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDTHFMAKIMANIRDFQSNVRKAQRLAKTSVPNEIETDVKADISRFQRALQRAKSMAQRWREHSVKLFMKTDEYKANLERAKAQVERFKQHKVDLKLSNTELMAKYNATKATVEAWRKHVVKLDLDANPAKMAVKGFKEDLIDLSRHSFDIDSSRWKLGNKFTKEFNEVEGAVKRSFGRIGQIMRKEVNGTSDIWGKLNNSLKDYGEKMDALATKIRTFGTIFAQQVKGLMIASIQALIPVIAGLVPAIMAVLNAVGVLGGGVLGLVGAFSVAGLGVVGFGAMAISALKMVEDGTLAVTKEVQNFRDASDQLKTTWRDIVKENQASIFNAMSAGIRGVTSAMSQLKPFLSEVSMLVEANARKFEDWVKHSETTKKAFEALNSIGGAIFGDLLNAAGRFGDGLINIFTQLMPLFKFVSQGLQNMSIAFQNWANSVAGQNAIKAFIDYTTTNLPKIGQIFGNVFAGIGNLMIAFAQNSSNIFDWLVKLTSQFRAWSEQVGQSQGFKDFISYVQENGPTIMQLIGNIVKALVAFGTAMAPIASKLLDFVTNLAGFIAKLFEAHPAVAQIIGVIGILGGVFWALMAPIAAVSSVLSNVFSMTLLNVVKRILDLTRITGVVSKAFGLLTGAFTSISWPILAVVAVIGVFIGILVYLWKTNENFRKTITEAWNGIKTAVSGAIQGVVDWLTQLWGKIQSTLQSIMPILQVLGQVFMQVLGVLVIGIITNVMNIIQGLWTLITIAFQAIGTVISVAVQIIVGLFTALIQLLTGDFSGAWETIKTTVTNVLDTIWQYMQSVWESIIGFLTGVMNRTLSMFGTSWSQIWSTITNFVSSIWSTVTSWFSRVAWSVAEKMGQALNFIITKGSEWVSNIWNTVTSFASKVADGFKRVVSNVGDGMSDALGKIKSFFSDFLNAGAELIGKVAEGVANAAHKVVSAVGDAISSAWDSVTSFVSGHGGGSSLGKGLAVSQAKVIATDFGSAFNKELSSTLTDSIVNPVSTSIDRHMTSDVQHSLKENNRPIVNVTIRNEGDLDLIKSRIDDMNAIDGSFNLL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]