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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0644 [new locus tag: NWMN_RS03660 ]
  • pan locus tag?: SAUPAN002559000
  • symbol: NWMN_0644
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0644 [new locus tag: NWMN_RS03660 ]
  • symbol: NWMN_0644
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 727475..728164
  • length: 690
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAAGTACTGCGCAATTAACAAAAGAAAATAATGTTAAATCATTAAGATTAAGCAAT
    ACTGATAGAGAAATCTTCGAGAATTATATGACTTATATGCGCTCTGACTTTCGCGTCAAC
    CCTCATGATACAGAGTTAATTATTAACCGTATACTCAAACAATTATTAAGCGCAGAACAA
    CATGGTTTATTAGCCTTAGATTTTTTCAATCACGACCCAAAAGCACATGCAATAAAAGAA
    CTAAAAGCGATGCCTAATGAAACATTTAAAAATATTTTTAAATATATCTATCAACACATC
    GTTCTATTAATTGGGATTGTTAGTTTTCTCAAAGGATTTTTAGGATTTTTCATGGAAAAA
    AATGGAAGTAATTTATATTTTGTTTCATTCCCGTTTTCAGTAGTCGTTGGATTCTTTATT
    GTCTTTTTATTTATATGGTTTAGTTTTAAAACAATACAACTGCAGTGTTTTAACAATTCA
    AATTGGATATGGATATTCACCTATTTAGCAATCATTCTACTAATTGTAGGATTTTTCTAT
    GTATTCTTTATTCCACAATCATTACTTGCCTTTGGACCATATATTCAGGTAAGCAATTGG
    GTATTTATTATATTTTCATTTATAGTTATGCCTATTGGATTACGCATTGAAAGAAATATT
    TCGAAAAAACATTCTCATACATTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0644 [new locus tag: NWMN_RS03660 ]
  • symbol: NWMN_0644
  • description: hypothetical protein
  • length: 229
  • theoretical pI: 9.95134
  • theoretical MW: 27033.7
  • GRAVY: 0.40917

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 33.5)
    and 3 more
    no clan defined UPF0767; UPF0767 family (PF15990; HMM-score: 12.5)
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 8.5)
    no clan defined DUF373; Domain of unknown function (DUF373) (PF04123; HMM-score: 5.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004599
    • TAT(Tat/SPI): 0.000169
    • LIPO(Sec/SPII): 0.000259
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDTELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVLLIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNSNWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIERNISKKHSHTFL

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]