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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
  • pan locus tag?: SAUPAN003428000
  • symbol: NWMN_1077
  • pan gene symbol?: ssl14
  • synonym:
  • product: superantigen-like protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
  • symbol: NWMN_1077
  • product: superantigen-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1180425..1181150
  • length: 726
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAAAGAACATCATGAATAAATTAGTTTTATCAACAGCATTGTTACTTTTAGAAACT
    ACATCAACACAACTTCCTAAAACACCAATCAGTTTTTCATCTGAAGCAAAAGCCTATAAT
    ATCAGTGAAAACGAGACTAATATCAATGAACTAATCAAATATTACACTCAGCCGCATTTT
    TCATTATCTGGAAAATGGTTATGGCAAAAGCCCAATGGTAGCATTCATGCAACATTGCAA
    ACGTGGGTTTGGTATAGTCATATTCAAGTGTTTGGATCCGAGAGTTGGGGAAACATTAAT
    CAGTTAAGAAATAAATACGTTGATATATTTGGAACTAAAGATGAGGACACAGTTGAAGGT
    TACTGGACTTATGATGAAACATTTACTGGTGGTGTTACGCCAGCAGCTACTTCATCTGAT
    AAGCCTTATAGACTATTTTTAAAATATAGTGATAAACAACAAACTATCATCGGTGGACAT
    GAATTTTACAAAGGAAATAAACCAGTATTAACTTTAAAAGAATTAGATTTCCGTATTCGT
    CAAACATTAATAAAAAATAAAAAGTTATATAACGGAGAATTTAATAAAGGTCAAATTAAG
    ATAACTGCTGATGGAAATAATTACACGATTGATTTAAGTAAAAAGTTAAAATTAACTGAC
    ACAAACCGTTATGTTAAAAATCCTCGTAATGCAGAAATTGAAGTCATACTCGAAAAATCT
    AACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    726

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1077 [new locus tag: NWMN_RS06105 ]
  • symbol: NWMN_1077
  • description: superantigen-like protein
  • length: 241
  • theoretical pI: 9.74236
  • theoretical MW: 27794.3
  • GRAVY: -0.641079

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 32.5)
    and 2 more
    no clan defined Dicty_CAD; Cell-cell adhesion domain (PF05720; HMM-score: 13.8)
    Lsr2; Lsr2 (PF11774; HMM-score: 13.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.01
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: PISFSSEA
  • SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.877821
    • TAT(Tat/SPI): 0.005262
    • LIPO(Sec/SPII): 0.073188
    • Cleavage Site: CS pos: 38-39. AKA-YN. Pr: 0.3634
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISENETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQLRNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGHEFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKLTDTNRYVKNPRNAEIEVILEKSN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Ilse Jongerius, Jörg Köhl, Manoj K Pandey, Maartje Ruyken, Kok P M van Kessel, Jos A G van Strijp, Suzan H M Rooijakkers
Staphylococcal complement evasion by various convertase-blocking molecules.
J Exp Med: 2007, 204(10);2461-71
[PubMed:17893203] [WorldCat.org] [DOI] (P p)