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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1309 [new locus tag: NWMN_RS07370 ]
  • pan locus tag?: SAUPAN003811000
  • symbol: hipO
  • pan gene symbol?: dapI
  • synonym:
  • product: hippurate hydrolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1309 [new locus tag: NWMN_RS07370 ]
  • symbol: hipO
  • product: hippurate hydrolase
  • replicon: chromosome
  • strand: +
  • coordinates: 1441538..1442689
  • length: 1152
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATGAATTAGAATTTGTTACGAAACATCGCCGTCATTTACATCAACATCCTGAATTA
    AGCTTACATGAATTTGAAACAACTGCTTATATTAAAGCGTTTTTAGATAGTTTAAATATT
    AAATACGATTGCCCATTGGAAACTGGCGTCATTGCATACTTAGAAGGTAATGGCTCACAT
    ACGATAGCGTATAGAGCTGATATTGATGCGTTACCTATTTTAGAGGAAAATGATGTGCCT
    TATCGCAGTCAATCTGATCATGTGATGCATGCTTGTGGACATGATGGTCATACAACTGCA
    TTAATGCTTTTTGTACAACGTTGCAAAGACATGCAAGATGCAGGTCAATTACCGCAAAAT
    GTCGTTTTCATTTTCCAACCTGCAGAAGAAACTGGTGGCGGTGCAAATCGATTAATAAAA
    GCCGGTGCCTTTGATAAGTATCCAATTGAAGCGGTATTTGGTATTCATGTTAACCCATTT
    GCTGATGAAGGCATTGCAGTGATAAGAGATGAAGAAATTACGGCCAGCGCAACAGAGTAT
    CGCTTTTTCTTAACAGGCCTGTCAAGTCATGTTGCTGATAAAGAACAAGGTCATTCTTGT
    GGTGAAGCATTACAACATGTATTAACTCAAATATCACAAATTCAACAATTTCACCTTAAC
    GGTTTGAAACGAAATATTGTTCATATTGGTCATTTTAAAGCTGGTGAAGCGATTAACACT
    GTACCAAGTAATGGCTATTTAGAAGGTACTATTCGTACATATGATATTGATGATTTAACA
    ATCGTTAAAAATCAAATGCACAAGATAGCAGAAAGTGTCAAGCTTCTGTTTAATGTAGAT
    TGTGAAGTTAAATTTGCAGAAGGTTATCCCCCTACAATCAATAGTCCGAAATTACGTACT
    CAAATAGAGGACGCCTTAATAAAAGCTGATTTAAATGTCTATGACAAACCAACGCCATTC
    TTATTTGGGGAAGATTTTAGTTTTTATGGTCAACAACTAGCTCCAGCTTACTTTGTTTTT
    ATAGGAACACGAAATGAAGATAAAGGTTTTGTAACTGGTTTGCACACATCACATTTAAAT
    TTTGATGAAAAAGTGTTAATAAACGTGGTTAATTTTTACGAAAATTTATTAAATAATTAC
    AAAGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1152

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1309 [new locus tag: NWMN_RS07370 ]
  • symbol: HipO
  • description: hippurate hydrolase
  • length: 383
  • theoretical pI: 5.35281
  • theoretical MW: 43143.3
  • GRAVY: -0.252219

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 279.6)
    and 2 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 20.7)
    Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 20.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 117.3)
    and 2 more
    no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 22.7)
    DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 13.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002731
    • TAT(Tat/SPI): 0.000328
    • LIPO(Sec/SPII): 0.000364
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDCPLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALMLFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPFADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFHLNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLLFNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLAPAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: L-box (transcription termination) regulon, CodY (repression) regulon
    L-box(RNA)important in Lysine biosynthesis; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]