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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
  • pan locus tag?:
  • symbol: NWMN_1769
  • pan gene symbol?:
  • synonym:
  • product: phage amidase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
  • symbol: NWMN_1769
  • product: phage amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1980874..1982328
  • length: 1455
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTAATGACAAAAAATCAAGCAGAAAAATGGTTTGACAATTCATTAGGGAAACAATTC
    AACCCAGATGGTTGGTATGGATTTCAGTGTTATGATTACGCCAATATGTTCTTTATGTTA
    GCGACAGGCGAAAGGCTGCAAGGTTTATATGCTTATAATATCCCGTTTGATAATAAAGCA
    AAGATTGAAAAATATGGTCAAATAATTAAAAACTATGACAGCTTTTTACCGCAAAAGTTG
    GATATTGTCGTTTTCCCGTCAAAGTATGGTGGCGGAGCTGGACACGTTGAAATTGTTGAG
    AGCGCAAATTTAAATACTTTCACATCATTTGGTCAAAACTGGAACGGTAAAGGTTGGACT
    AATGGCGTTGCGCAACCTGGTTGGGGTCCTGAAACTGTGACAAGACATGTTCATTATTAT
    GACAATCCAATGTATTTTATTAGGTTAAACTTCCCTAACAACTTAAGCGTTGGCAATAAA
    GCTAAAGGTATTATTAAGCAAGCGACTACAAAAAAAGAGGCAGTAATTAAACCTAAAAAA
    ATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTAGGAAACGGAACAAAC
    GAACGCGATTTTATACGTAAATATATAACGCCTAATATCGCTAAGTATTTAAGACATGCA
    GGACATGAAGTTGCATTATACGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACT
    GCATACGGTGTTAATGTAGGCAATAAAAAAGATTATGGCTTATATTGGGTTAAATCACAG
    GGGTATGACATTGTTCTAGAAATACATTTAGACGCAGCAGGAGAAAGCGCAAGTGGTGGG
    CATGTTATTATCTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTT
    ATTAAAAATAACTTAGGACAAATAAGAGGTGTGACACCTCGTAATGATTTACTAAATGTT
    AATGTATCAGCAGAAATAAATATAAATTATCGTTTATCTGAATTAGGTTTTATTACTAAT
    AAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGT
    GCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAA
    AACAAAAAAAATCCACCAGTGCCAGCAGGTTATACACTCGATAAGAATAATGTCCCTTAT
    AAAAAAGAACAAGGCAATTACACAGTAGCTAATGTTAAAGGTAATAATGTAAGAGACGGT
    TATTCAACTAATTCAAGAATTACAGGGGTATTACCCAACAACACAACAATTACGTATGAC
    GGTGCATATTGTATTAATGGTTATAGATGGATTACTTATATTGCTAATAGTGGACAACGT
    CGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAG
    TTTAGCACGATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1455

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1769 [new locus tag: NWMN_RS10115 ]
  • symbol: NWMN_1769
  • description: phage amidase
  • length: 484
  • theoretical pI: 9.70541
  • theoretical MW: 53800.2
  • GRAVY: -0.509711

Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
    Cellular processes Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 43.8)
  • TheSEED:
  • PFAM:
    Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 136.8)
    and 3 more
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 37.5)
    SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 35.6)
    SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 17.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022393
    • TAT(Tat/SPI): 0.000344
    • LIPO(Sec/SPII): 0.001173
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLMTKNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNKAKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNKKNPPVPAGYTLDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]