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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0901 [new locus tag: SA_RS05110 ]
  • pan locus tag?: SAUPAN003251000
  • symbol: sspA
  • pan gene symbol?: sspA
  • synonym:
  • product: serine protease; V8 protease; glutamyl endopeptidase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0901 [new locus tag: SA_RS05110 ]
  • symbol: sspA
  • product: serine protease; V8 protease; glutamyl endopeptidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1021647..1022675
  • length: 1029
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGTTGCAACTTTGACAACAGCGACA
    CTTGTGAGTTCTCCAGCAGCAAACGCGTTATCATCAAAAGCTATGGACAATCATCCACAA
    CAAACGCAGTCAAGCAAACAGCAAACACCTAAGATTAAAAAAGGCGGTAACCTTAAACCA
    TTAGAACAACGTGAACACGCAAATGTTATATTACCGAATAACGATCGTCACCAAATCACA
    GATACAACGAATGGTCATTATGCACCCGTAACTTATATTCAAGTTGAAGCACCTACTGGT
    ACATTTATTGCTTCCGGTGTAGTTGTAGGTAAAGATACTCTTTTAACAAATAAACACGTC
    GTAGATGCTACGCACGGTGATCCTCATGCTTTAAAAGCATTCCCTTCTGCAATTAACCAA
    GACAATTATCCAAATGGTGGTTTCACTGCTGAACAAATCACTAAATATTCAGGCGAAGGT
    GATTTAGCAATAGTTAAATTCTCCCCTAATGAGCAAAACAAACATATTGGTGAAGTAGTT
    AAACCAGCAACAATGAGTAATAATGCTGAAACACAAGTTAACCAAAATATTACTGTAACA
    GGATATCCTGGTGATAAACCTGTAGCAACAATGTGGGAAAGTAAAGGAAAAATCACTTAC
    CTCAAAGGCGAAGCTATGCAATATGATTTAAGTACAACTGGTGGTAACTCAGGTTCACCT
    GTATTTAATGAAAAAAATGAAGTGATCGGAATTCATTGGGGCGGTGTACCAAATGAATTT
    AATGGTGCGGTATTTATTAATGAAAATGTACGCAACTTCTTAAAACAAAATATTGAAGAT
    ATCCATTTTGCCAACGATGACCAACCTAATAACCCAGATAATCCTGATAACCCTAACAAT
    CCTGATAACCCTAACAATCCTGATAACCCTAACAACCCAGATGAACCAAATAACCCTGAC
    AACCCTAACAACCCTGATAATCCAGACAATGGCGATAACAATAATTCAGACAACCCTGAC
    GCTGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0901 [new locus tag: SA_RS05110 ]
  • symbol: SspA
  • description: serine protease; V8 protease; glutamyl endopeptidase
  • length: 342
  • theoretical pI: 4.81891
  • theoretical MW: 36977.1
  • GRAVY: -0.876608

Function[edit | edit source]

  • reaction:
    EC 3.4.21.19?  ExPASy
    Glutamyl endopeptidase Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.7)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.7)
    and 2 more
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.2)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 13.2)
  • TheSEED  :
    • Glutamyl endopeptidase (EC 3.4.21.19), serine proteinase SspA
    Protein Metabolism Protein degradation EC 3.4.21.- Serine endopeptidase  Glutamyl endopeptidase precursor (EC 3.4.21.19), serine proteinase SspA
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 75)
    and 7 more
    Trypsin; Trypsin (PF00089; HMM-score: 50.6)
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 26.8)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 22.1)
    Peptidase_C3; 3C cysteine protease (picornain 3C) (PF00548; HMM-score: 18.3)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 17.1)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 16.1)
    Peptidase_CA (CL0125) Phytochelatin; Phytochelatin synthase (PF05023; HMM-score: 11.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: VSSPAANA
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.9677
    • TAT(Tat/SPI): 0.027189
    • LIPO(Sec/SPII): 0.0042
    • Cleavage Site: CS pos: 29-30. ANA-LS. Pr: 0.6942
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIKKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPDAA

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]