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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1675 [new locus tag: SA_RS09465 ]
  • pan locus tag?: SAUPAN004794000
  • symbol: SA1675
  • pan gene symbol?: glnP
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1675 [new locus tag: SA_RS09465 ]
  • symbol: SA1675
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1911446..1912771
  • length: 1326
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    GTGGGTCTTTCTGCAGATTATGCACCAATGGAATTTGAGCATACAGTTAATGGTAAGACT
    GAGTATGCAGGTGTAGATATTGATTTAGCTAAAAAAATTGCGAAAGATAATAATTTAAAA
    TTAAAAATCGTCAATATGTCATTTGATAGTTTGTTAGGAGCTCTTAAAACTGGAAAAATT
    GATATTATTATTTCCGGAATGACTTCAACGCCTGAACGTAAGAAGCAAGTTGATTTTTCA
    GATTCATATATGATGACTAAAAATATCATGCTTGTAAAGAAAGATAAAGTTAATGAATAT
    AAAGATATCAAAGACTTTAATAATAAAAAAGTAGGGGCACAAAAGGGAACTGAACAAGAA
    AAAATCGCTCAAACCGAAATTGAAAATGCATCTATTACTTCATTAAGCCGTTTACCAGAC
    GTTATATTAGCATTGAAAAGTGGAAAGGTTGAAGGTGCTGTAGTTGAAAAACCTGTAGCA
    GAAGCATATTTAAAACAAAATCCTAAATTAGGAATTTCCAATGTGAAATTTAATGAAGAA
    GAAAAAGATACAGTGATAGCTGTGCCAAAAGATTCACCAAAATTATTGTCACAAATTAAT
    AAAACGATTAAAGAGGTTAAAGATAAAGGATTAATCGATAAATATATGACTAATGCTGCA
    AATGCGATGAATGATGACAGTGGTTTTATTTCTAAGTATGGTAGTTTTTTCTTGAAAGGA
    ATTAAGATTACAATATTAATTTCACTTATCGGTGTTGCATTAGGTTCTATTTTAGGTGCA
    TTCGTTGCGTTAATGAAATTAAGTAAAATAAAAATTATTTCATGGATTGCTTCTATCTAT
    ATCGAAATATTAAGAGGAACACCAATGTTAGTTCAAGTATTTATCGTATTCTTTGGTATA
    ACTGCTGCATTAGGACTAGACATTTCAGCTCTTGTTTGTGGAACAATTGCATTAGTTATT
    AATTCCTCAGCTTACATTGCTGAAATTATTCGTGCAGGTATAAATGCTGTTGATAAAGGA
    CAAATGGAAGCCGCACGTAGTCTAGGTTTGAATTATAGACAAACGATGAAAAGTGTAATT
    ATGCCACAAGCAATTAAAAATATTTTACCAGCTTTAGGTAATGAATTTGTCACTTTAATT
    AAAGAATCATCTATTGTGTCAACAATTGGAGTTGGCGAAATTATGTTTAATGCACAAGTG
    GTTCAAGGTATTTCATTTGACCCATTCACACCATTACTAGTGGCAGCAGCATTATACTTT
    GTCTTAACATTTGTACTTACACGCATTATGAACATGATTGAAGGGAGATTGAATGCCAGT
    GATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1326

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1675 [new locus tag: SA_RS09465 ]
  • symbol: SA1675
  • description: hypothetical protein
  • length: 441
  • theoretical pI: 9.59061
  • theoretical MW: 48056.2
  • GRAVY: 0.217234

Function[edit | edit source]

  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 159)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 153.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines lysine-arginine-ornithine-binding periplasmic protein (TIGR01096; HMM-score: 128.2)
    and 12 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 98.7)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 46.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ectoine/hydroxyectoine ABC transporter solute-binding protein EhuB (TIGR02995; HMM-score: 43.6)
    extracellular substrate-binding orphan protein, GRRM family (TIGR04262; HMM-score: 38)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 30.1)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 23)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 23)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 18.7)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 18.3)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR02285; HMM-score: 14.8)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 12.6)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 12.2)
  • TheSEED  :
    • Arginine ABC transporter, permease protein
    • Arginine ABC transporter, substrate-binding protein ArgBP
  • PFAM:
    PBP (CL0177) SBP_bac_3; Bacterial extracellular solute-binding proteins, family 3 (PF00497; HMM-score: 186)
    and 4 more
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 85.4)
    PBP (CL0177) Lig_chan-Glu_bd; Ligated ion channel L-glutamate- and glycine-binding site (PF10613; HMM-score: 37.9)
    NMT1; NMT1/THI5 like (PF09084; HMM-score: 17)
    no clan defined Bravo_FIGEY; Bravo-like intracellular region (PF13882; HMM-score: 12.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00777
    • TAT(Tat/SPI): 0.000337
    • LIPO(Sec/SPII): 0.000666
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTGKIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKGTEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISNVKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISKYGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPMLVQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSLGLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISFDPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: ArgR* (repression) regulon, CodY (repression) regulon
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]