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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1995 [new locus tag: SA_RS11485 ]
  • pan locus tag?: SAUPAN005589000
  • symbol: lacC
  • pan gene symbol?: lacC
  • synonym:
  • product: tagatose-6-phosphate kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1995 [new locus tag: SA_RS11485 ]
  • symbol: lacC
  • product: tagatose-6-phosphate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2267122..2268054
  • length: 933
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTTTAACTTTAACATTAAATCCATCCGTTGATATTTCATATCCATTAACAGCATTG
    AAATTAGATGATGTTAATCGTGTTCAAGAAGTAAGTAAAACAGCTGGTGGTAAAGGTTTA
    AATGTAACAAGAGTACTTGCGCAAGTAGGCGAGCCAGTCCTTGCGAGTGGTTTTATTGGC
    GGTGAATTAGGTCAATTTATTGCTAAAAAATTAGATCATGCCGACATCAAGCATGCATTT
    TATAACATTAAGGGTGAAACACGAAACTGCATTGCAATTTTACATGAAGGGCAACAAACA
    GAAATTTTAGAGCAGGGACCGGAGATTGATAATCAAGAGGCTGCAGGATTTATTAAACAT
    TTTGAACAATTATTAGAAAAAGTTGAAGCAGTTGCTATTTCAGGTAGCTTACCTAAAGGA
    TTAAATCAAGATTATTATGCACAAATTATTGAACGATGCCAAAACAAAGGTGTGCCTGTG
    ATATTGGACTGCTCGGGTGCAACTTTGCAAACAGTTTTGGAAAATCCATACAAACCAACA
    GTCATTAAACCAAACATTTCTGAACTATATCAATTGCTTAACCAGCCACTTGATGAAAGT
    TTAGAAAGTTTGAAACAGGCAGTGTCACAACCCTTATTCGAAGGGATTGAGTGGATTATT
    GTGTCTTTAGGTGCACAAGGTGCATTCGCTAAGCACAATCATACATTCTATAGAGTGAAT
    ATTCCGACAATAAACGTATTAAATCCGGTTGGTTCAGGTGATTCTACAGTTGCTGGAATT
    ACTTCAGCAATTCTCAATCATGAAAACGACCACGATTTACTAAAAAAAGCTAATACTTTA
    GGCATGCTGAATGCGCAAGAAGCACAAACAGGATATGTCAATTTAAATAATTATGATGAA
    TTATTCAATCAAATTGAAGTTTTAGAGGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1995 [new locus tag: SA_RS11485 ]
  • symbol: LacC
  • description: tagatose-6-phosphate kinase
  • length: 310
  • theoretical pI: 4.66537
  • theoretical MW: 33894.3
  • GRAVY: -0.106452

Function[edit | edit source]

  • reaction:
    EC 2.7.1.144?  ExPASy
    Tagatose-6-phosphate kinase ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 618.3)
    and 7 more
    hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 324.1)
    1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 279.9)
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 65.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 60.2)
    Metabolism Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 48.5)
    Unknown function General YjeF family C-terminal domain (TIGR00196; HMM-score: 40.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 11.9)
  • TheSEED  :
    • Tagatose-6-phosphate kinase (EC 2.7.1.144)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose-6-phosphate kinase (EC 2.7.1.144)
  • PFAM:
    Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 218.6)
    and 2 more
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 38.6)
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 20.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013235
    • TAT(Tat/SPI): 0.000863
    • LIPO(Sec/SPII): 0.000994
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTRVLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEILEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPVILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEWIIVSLGAQGAFAKHNHTFYRVNIPTINVLNPVGSGDSTVAGITSAILNHENDHDLLKKANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: LacR (repression) regulon
    LacR(TF)important in Lactose utilization; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]