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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2160 [new locus tag: SA_RS12400 ]
  • pan locus tag?: SAUPAN005886000
  • symbol: SA2160
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2160 [new locus tag: SA_RS12400 ]
  • symbol: SA2160
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2427313..2428317
  • length: 1005
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGATAGAAACATTTAAAGCGTTTGTAATTGATAAAGATGAGAGCGGTAAAGTGACACCA
    ACTTTCAAACAATTATCGCCTACTGATTTACCTAAAGGAGATGTGCTGATTAAAGTACAT
    TACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGATCATAATGCAGTCGTAAAA
    TCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATTGTTGAATCCGAAGCACCA
    GGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGACCTAGGTGTTAGCCATTAT
    GGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATTATCAAGCTTCCTGATACT
    TTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTATACTGCCGGTTTAGCAATT
    GAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCTGTACTCGTTCGCGGTGCT
    TCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAACTTGGTTATAAAGTTATC
    GCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAACTTGGTGCCAAAGAAGTT
    ATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTCGCATCATCAACTTGGCAA
    GCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTTACAAAACGTTTAAATCAT
    AGTGGATCAATTGCAGTTATTGGTATGACTGCCGGCAATACTTATACTAATTCTGTATTC
    CCTCACATTTTAAGAGGCGTAAACATTTTAGGAATTGACTCAGTATTTACTGCTATGAAA
    TTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAAAGCCTGAAAATTTACATGAG
    ATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTTAACAAAGTAATTAATCAT
    GAAAATAAAGGCCGCATTGTTATCGATTTCGGTGTAGATAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2160 [new locus tag: SA_RS12400 ]
  • symbol: SA2160
  • description: hypothetical protein
  • length: 334
  • theoretical pI: 5.65303
  • theoretical MW: 36552.6
  • GRAVY: -0.127545

Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 452)
    and 8 more
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 95.8)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 57.8)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 38.5)
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.4)
    Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 30.9)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 26.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 25.2)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 12.8)
  • TheSEED  :
    • Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84)
    Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
    and 2 more
    Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 46.7)
    and 4 more
    GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 34.5)
    NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 16.6)
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 13.6)
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004128
    • TAT(Tat/SPI): 0.000424
    • LIPO(Sec/SPII): 0.000657
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]