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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2476 [new locus tag: SA_RS14170 ]
  • pan locus tag?: SAUPAN006440000
  • symbol: SA2476
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2476 [new locus tag: SA_RS14170 ]
  • symbol: SA2476
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2787227..2788834
  • length: 1608
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    GTGCTAGTAGTTGGTGCTTCAGGTAGTGGTAAATCGACTTTTGCAAATTGCATAAACGGA
    TTAATTCCATTTAAAACTAAAGGTAACATAACCGGGGAACTATATATAAATAATCAAGAT
    GCAACCGTTAGTTGTTTACATGATAGATCTAATGTTGTTGGTACAGTTTTACAAGATACA
    GATGGACAGTTCATAGGCTTAACAGCAGCTGAAGATATGGCCTTTTTATTAGAAAATAAT
    TGTGTTGAACAAGATGATATGAAGAAAAATGTAAGTTATTGGGCTGAAAAAGTTGGCATG
    ATAGAACATTTAAATCACCGACCGCAAGATTTATCTGGAGGTCAAAAACAACGCGTTTCA
    TTAGGTGGTATATTAATCCATCGTACGCCTATTTTAATATTGGATGAGCCGCTGGCCAAT
    TTAGATCCTGCGACAGGACATGAAACGCTGAGATTGTTAAACAATATTCATGAAGAAACA
    AAGTCAACGATGATTATTGTCGAACATCGATTAGAAGAATCATTAGATGATACGTTTGAT
    AGAGTTCTTCTTTTTAAAGATGGAAAAATCATCGCTAATACAACGCCTAGTGATTTGCTA
    AAATCATCTAAGCTTAAAGAAGCAGGCATACGTGAACCATTATACTGTACAGCGCTTAAA
    TATGCAGAAGTAGATGTCGAATCCATTGATAACTTAGCGAATTTACGTGACGTTTGTATG
    AGTGAACATGTTAAATTTAAAGTGAAAAAATGGATAGATGAAACATCTGCAAATAATGAT
    AATAAGTACAAATCTGAACCGCTATTAGAATTAAATGAGGTATGTGTTCAATATAGTGAC
    TATAGTAATAGTGTACTTAATAATGTTCAATTAAATGTTTATAGAAGAGAAATGCTTAGT
    ATAGTTGGTCACAATGGTGCCGGCAAATCAACACTTGCTAAAGCAATTTGTGGATTTTTA
    GATATAACAGGTAATATCCAATTTTGTAATAGGGGATTTAATCAATTGTCAATTAGCGAA
    CGATCTGAATTCGTAGGTTATGTGATGCAAAATCCAAATCATATGATTTCTGAAAAAATG
    ATTTACGATGAAGTAGCATTAGGGTTAAGAGCACGAGGTATGAAAGAATCAGATATAAAG
    ATACGAGTAGAGAATGTGCTCAAAATATGCGGACTTTATGCATTTCGGAATTGGCCAATT
    GCAGCTTTAAGCTATGGTCAAAAAAAGAGGGTCACTATAGCATCTGTTTTAGTCTTAAAT
    CCGGAAATAATCATATTGGATGAACCGACTGCTGGTCAAGATTTCTATCATTATAATGAG
    ATAATGTCATTTTTAATTGAGCTAAACAGACAGGGAAAGACGATTATTATGATTACGCAT
    GATATGCATTTATTGTCTGAGTATAGTTCAAGAACAGTTGTATTATCAAAAGGACAAGTC
    GTTGCTGATACCACGCCAGTATTGATATTAAATGATAAAAAAATCTGTGAGATTGCATCA
    TTGAGACAAACATCGCTATTTGAAATGGCCGAATATATAGGGATTAGCGAGCCACAGAAA
    TTAGTACAATTATTTATTAACCATGATAGGAAGGTGAGACGCCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1608

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2476 [new locus tag: SA_RS14170 ]
  • symbol: SA2476
  • description: hypothetical protein
  • length: 535
  • theoretical pI: 6.16625
  • theoretical MW: 60147.6
  • GRAVY: -0.193271

Function[edit | edit source]

  • reaction:
    EC 3.6.3.-?  ExPASy
  • TIGRFAM:
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 358.2)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 321.2)
    and 82 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 234.8)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 228.4)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 204.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 197.8)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 194.4)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 191.3)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 191.3)
    Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 188.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 188.6)
    Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 185.6)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 185.2)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 184.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 183.8)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 181.8)
    Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 180.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 180.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 179.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 175.4)
    Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 175.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 173.6)
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 172.4)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 172.3)
    D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 171.3)
    gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 166.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 163.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 161.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 160.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 158.3)
    ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 156.8)
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 153.3)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 151.2)
    Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 151.2)
    Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 151.2)
    thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 151)
    Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 150.6)
    proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 149.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 145.4)
    lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 144.8)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 144.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 144.1)
    Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 136.6)
    Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 136.6)
    Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 133.6)
    Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 133.6)
    Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 131.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 129.9)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 129.9)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 129.4)
    Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 129.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 129.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 121.4)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 115.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 115.3)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 115)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 112.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 111.4)
    ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 107.6)
    Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 105.3)
    Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 101.9)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 101)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 99)
    Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 99)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 93.4)
    Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 92.8)
    Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 68.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 62.2)
    Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 53.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 52.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 51.9)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 19.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA replication and repair protein RecF (TIGR00611; HMM-score: 17.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uridine kinase (TIGR00235; EC 2.7.1.48; HMM-score: 15.3)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 13.4)
    Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 13.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 12.8)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage/plasmid primase, P4 family, C-terminal domain (TIGR01613; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 12.1)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 11.9)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 11.6)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 10.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcC (TIGR00618; HMM-score: 10.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 10.2)
  • TheSEED  :
    • Methionine-regulated ECF transporter, duplicated ATPase component of energizing module MtsB
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter
    and 1 more
    Membrane Transport Membrane Transport - no subcategory ECF class transporters  Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter
  • PFAM:
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 163.2)
    and 36 more
    no clan defined DUF3744; ATP-binding cassette cobalt transporter (PF12558; HMM-score: 72.4)
    P-loop_NTPase (CL0023) AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 51.6)
    SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 46.6)
    AAA_23; AAA domain (PF13476; HMM-score: 38.6)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 32.5)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 29.9)
    AAA_22; AAA domain (PF13401; HMM-score: 29.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 25.7)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 23.4)
    AAA_33; AAA domain (PF13671; HMM-score: 23.4)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 22)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 21.2)
    Mg_chelatase; Magnesium chelatase, subunit ChlI (PF01078; HMM-score: 20.6)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.7)
    AAA_18; AAA domain (PF13238; HMM-score: 19.5)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 18.3)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 17.8)
    NACHT; NACHT domain (PF05729; HMM-score: 17.5)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 17)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 17)
    DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 16)
    Sigma54_activat; Sigma-54 interaction domain (PF00158; HMM-score: 15.9)
    AAA_27; AAA domain (PF13514; HMM-score: 14.8)
    AAA_24; AAA domain (PF13479; HMM-score: 14.7)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 14.6)
    FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 14.2)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 14.1)
    Septin; Septin (PF00735; HMM-score: 13.6)
    SbcCD_C; Putative exonuclease SbcCD, C subunit (PF13558; HMM-score: 13.3)
    AAA_13; AAA domain (PF13166; HMM-score: 12.7)
    PRK; Phosphoribulokinase / Uridine kinase family (PF00485; HMM-score: 12.5)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 12.3)
    AAA_25; AAA domain (PF13481; HMM-score: 12.1)
    ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 11.5)
    AAA_30; AAA domain (PF13604; HMM-score: 11.3)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 8.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.067465
    • TAT(Tat/SPI): 0.001336
    • LIPO(Sec/SPII): 0.020965
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLVVGASGSGKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGLTAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGILIHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVLLFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMSEHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREMLSIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMISEKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIASVLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTVVLSKGQVVADTTPVLILNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRKVRRQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]