From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0663 [new locus tag: SACOL_RS03430 ]
  • pan locus tag?: SAUPAN002473000
  • symbol: argS
  • pan gene symbol?: argS
  • synonym:
  • product: arginyl-tRNA synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0663 [new locus tag: SACOL_RS03430 ]
  • symbol: argS
  • product: arginyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 690641..692302
  • length: 1662
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATATTATTGATCAAGTGAAACAAACATTAGTAGAAGAAATTGCAGCAAGTATTAAC
    AAAGCAGGATTAGCAGATGAGATTCCTGATATTAAAATTGAAGTTCCTAAAGATACAAAA
    AATGGAGATTATGCTACTAATATTGCGATGGTACTGACTAAGATTGCAAAGCGTAATCCT
    CGTGAAATTGCTCAAGCGATTGTTGATAACTTAGATACTGAAAAAGCACATGTAAAACAA
    ATTGACATTGCTGGTCCAGGATTCATTAATTTTTACTTAGATAATCAGTATTTAACAGCA
    ATTATTCCTGAAGCAATTGAAAAAGGTGATCAATTTGGACATGTAAATGAATCAAAAGGT
    CAAAATGTATTGCTTGAGTATGTTTCAGCTAACCCTACAGGAGATTTACATATTGGTCAT
    GCTAGAAATGCAGCAGTTGGTGATGCTTTAGCTAATATTTTAACTGCAGCTGGCTATAAT
    GTAACACGTGAATATTATATTAATGATGCTGGTAATCAAATTACTAACTTAGCGCGTTCG
    ATTGAAACACGTTTCTTTGAAGCTTTAGGTGACAATAGTTATTCAATGCCAGAAGATGGC
    TATAATGGAAAAGATATTATTGAAATAGGTAAAGATTTAGCAGAGAAACACCCTGAAATT
    AAAGATTATTCTGAAGAAGCACGTTTGAAAGAATTTAGAAAATTAGGCGTAGAATACGAA
    ATGGCTAAATTGAAAAATGATTTAGCAGAGTTCAATACGCATTTTGATAATTGGTTTAGT
    GAAACATCTTTATATGAAAAAGGAGAAATTCTTGAAGTTTTAGCAAAAATGAAAGAATTA
    GGTTATACGTATGAAGCTGATGGCGCTACATGGTTACGTACAACTGATTTTAAAGACGAC
    AAAGACAGAGTATTAATTAAAAATGACGGTACATATACGTATTTCTTACCAGATATTGCG
    TACCACTTCGATAAAGTAAAACGTGGTAATGACATTTTAATCGATTTATTTGGTGCTGAT
    CATCATGGTTATATTAATCGTTTGAAAGCATCTCTTGAAACGTTTGGTGTAGATAGTAAT
    CGTTTAGAAATTCAAATCATGCAAATGGTTCGTTTAATGGAAAATGGTAAAGAAGTGAAG
    ATGAGTAAACGTACTGGTAATGCGATTACATTAAGAGAAATTATGGACGAAGTTGGCGTT
    GACGCTGCACGTTATTTCTTAACTATGCGTAGTCCTGATAGTCACTTTGATTTTGATATG
    GAATTAGCGAAAGAGCAATCTCAAGACAATCCAGTTTACTATGCTCAATATGCACATGCG
    CGTATTTGTTCAATTTTAAAACAAGCGAAAGAGCAAGGTATTGAAGTGACTGCTGCGAAT
    GATTTTACAACGATTACTAATGAAAAAGCGATTGAATTGTTGAAAAAAGTAGCTGATTTC
    GAACCTACAATTGAAAGTGCTGCTGAGCATAGATCGGCACATAGAATTACTAATTATATT
    CAAGATTTAGCTTCTCATTTCCATAAATTCTATAATGCTGAAAAAGTGTTAACAGATGAT
    ATTGAAAAAACAAAAGCACATGTTGCTATGATTGAAGCGGTCAGAATTACATTGAAAAAT
    GCATTGGCAATGGTCGGTGTAAGCGCACCTGAATCAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1662

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0663 [new locus tag: SACOL_RS03430 ]
  • symbol: ArgS
  • description: arginyl-tRNA synthetase
  • length: 553
  • theoretical pI: 4.86205
  • theoretical MW: 62380.9
  • GRAVY: -0.425316

Function[edit | edit source]

  • reaction:
    EC 6.1.1.19?  ExPASy
    Arginine--tRNA ligase ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 511.7)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 42.6)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 41.6)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 37.1)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 33.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 31.4)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 22.7)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 20.7)
  • TheSEED  :
    • Arginyl-tRNA synthetase (EC 6.1.1.19)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Arg  Arginyl-tRNA synthetase (EC 6.1.1.19)
  • PFAM:
    DALR (CL0258) DALR_1; DALR anticodon binding domain (PF05746; HMM-score: 123.1)
    and 9 more
    no clan defined Arg_tRNA_synt_N; Arginyl tRNA synthetase N terminal domain (PF03485; HMM-score: 90.2)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 89.8)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 38.3)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 33.9)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 31.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 28)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 16.2)
    tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 15.7)
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006584
    • TAT(Tat/SPI): 0.000488
    • LIPO(Sec/SPII): 0.001017
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDYATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAIIPEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYNVTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHPEIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAKMKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILIDLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLREIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAKEQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFHKFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 1355 [4]
  • interaction partners:
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [5] (data from MRSA252)

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: 38.31 h [6]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]