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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2117 [new locus tag: SACOL_RS11075 ]
  • pan locus tag?: SAUPAN005417000
  • symbol: fbaA
  • pan gene symbol?: fbaA
  • synonym:
  • product: fructose-bisphosphate aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2117 [new locus tag: SACOL_RS11075 ]
  • symbol: fbaA
  • product: fructose-bisphosphate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2177627..2178487
  • length: 861
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCCTTTAGTTTCAATGAAAGAAATGTTAATTGATGCAAAAGAAAATGGTTATGCGGTA
    GGTCAATACAATATTAATAACCTAGAATTCACTCAAGCAATTTTAGAAGCGTCACAAGAA
    GAAAATGCACCTGTAATTTTAGGTGTTTCTGAAGGTGCTGCTCGTTACATGAGCGGTTTC
    TACACAATTGTTAAAATGGTTGAAGGGTTAATGCATGACTTAAACATCACTATTCCTGTA
    GCAATCCATTTAGACCATGGTTCAAGCTTTGAAAAATGTAAAGAAGCTATCGATGCTGGT
    TTCACATCAGTAATGATCGATGCTTCACACAGCCCATTCGAAGAAAACGTAGCAACAACT
    AAAAAAGTTGTTGAATACGCTCATGAAAAAGGTGTTTCTGTAGAAGCTGAATTAGGTACT
    GTTGGTGGACAAGAAGATGATGTTGTAGCAGACGGCATCATTTATGCTGATCCTAAAGAA
    TGTCAAGAACTAGTTGAAAAAACTGGTATTGATGCATTAGCGCCAGCATTAGGTTCAGTT
    CATGGTCCATACAAAGGTGAACCAAAATTAGGATTTAAAGAAATGGAAGAAATCGGTTTA
    TCTACAGGTTTACCATTAGTATTACACGGTGGTACTGGTATCCCGACTAAAGATATCCAA
    AAAGCAATTCCATTTGGTACAGCTAAAATTAACGTAAACACTGAAAACCAAATCGCTTCA
    GCAAAAGCAGTTCGTGACGTTTTAAATAACGACAAAGAAGTTTACGATCCTCGTAAATAC
    TTAGGACCTGCACGTGAAGCCATCAAAGAAACAGTTAAAGGTAAAATTAAAGAGTTCGGT
    ACTTCTAACCGCGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    861

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2117 [new locus tag: SACOL_RS11075 ]
  • symbol: FbaA
  • description: fructose-bisphosphate aldolase
  • length: 286
  • theoretical pI: 4.74859
  • theoretical MW: 30835.9
  • GRAVY: -0.228322

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.13?  ExPASy
    Fructose-bisphosphate aldolase D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 427.6)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis ketose-bisphosphate aldolase (TIGR00167; HMM-score: 379.5)
    and 3 more
    class II aldolase, tagatose bisphosphate family (TIGR01858; EC 4.1.2.-; HMM-score: 289.7)
    fructose-bisphosphate aldolase, class II, Calvin cycle subtype (TIGR01521; EC 4.1.2.13; HMM-score: 193)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-bisphosphate aldolase, class II (TIGR01520; EC 4.1.2.13; HMM-score: 104.2)
  • TheSEED  :
    • Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
    and 1 more
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
  • PFAM:
    TIM_barrel (CL0036) F_bP_aldolase; Fructose-bisphosphate aldolase class-II (PF01116; HMM-score: 341.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006066
    • TAT(Tat/SPI): 0.000257
    • LIPO(Sec/SPII): 0.00049
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAPVILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFTSVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKECQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKDIQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKIKEFGTSNRAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4] [5]
  • quantitative data / protein copy number per cell: 9719 [6]
  • interaction partners:
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [7] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [7] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [7] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [7] (data from MRSA252)
    SACOL0742hypothetical protein  [7] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 57.69 h [8]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  7. ↑ 7.0 7.1 7.2 7.3 7.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  8. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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