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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01009
  • pan locus tag?: SAUPAN003278000
  • symbol: SAOUHSC_01009
  • pan gene symbol?: purK
  • synonym:
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01009
  • symbol: SAOUHSC_01009
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 979791..980915
  • length: 1125
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACTTCAATAAATTAAAGTTTGGTGCGACTATTGGCATTATTGGTGGTGGTCAGCTT
    GGAAAGATGATGGCACAATCAGCTCAAAAAATGGGTTATAAAGTGGTTGTATTGGATCCT
    TCTGAAGATTGTCCATGTAGATACGTTGCACACGAATTTATACAAGCCAAGTATGACGAT
    GAAAAGGCACTCAATCAATTAGGACAAAAATGTGATGTGATTACTTATGAATTTGAAAAC
    ATTTCAGCCCAACAATTAAAACTATTATGTGAAAAGTACAATATTCCGCAAGGTTACCAA
    GCTATACAGTTATTACAAGATCGCTTAACTGAAAAAGAAACATTAAAAAGTGCTGGTACC
    AAAGTTGTCCCGTTCATTTCAGTAAAAGAATCTACAGATATTGACAAAGCAATTGAAACA
    TTAGGATATCCTTTTATTGTAAAAACTAGATTTGGTGGCTACGATGGCAAAGGTCAAGTT
    TTAATTAACAACGAAAAAGACTTACAAGAAGGTTTTAAATTAATTGAAACTAGTGAATGC
    GTAGCTGAAAAATATTTGAATATCAAGAAAGAAGTATCTCTTACTGTTACAAGAGGAAAC
    AACAATCAAATCACTTTTTTCCCATTACAAGAAAATGAGCATAGAAATCAAATACTTTTC
    AAAACAATTGTTCCAGCGAGAATAGATAAAACAGCTGAGGCGAAAGAGCAAGTTAATAAA
    ATTATCCAATCGATTCATTTCATTGGAACATTTACAGTTGAATTTTTTATAGATAGTAAC
    AACCAATTGTATGTGAACGAGATAGCACCAAGGCCTCACAATTCCGGACATTATTCAATT
    GAAGCATGTGATTATTCACAATTTGATACTCATATTTTAGCAGTTACCGGACAATCATTA
    CCAAATTCAATTGAATTATTAAAGCCAGCAGTCATGATGAACTTACTAGGTAAAGATTTA
    GATTTATTGGAAAATGAATTTAATGAACATCCAGAGTGGCACTTACATATTTATGGTAAG
    TCTGAGCGTAAAGATAGCAGAAAAATGGGGCATATGACTGTACTAACGAATGATGTAAAC
    CAAACTGAACAAGATATGTACGCTAAATTTGAGGGGAGTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

Protein Data Bank: 3ORQ
Protein Data Bank: 3ORR

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01009
  • symbol: SAOUHSC_01009
  • description: 5-(carboxyamino)imidazole ribonucleotide synthase
  • length: 374
  • theoretical pI: 5.8331
  • theoretical MW: 42493.1
  • GRAVY: -0.437968

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.21?  ExPASy
    Phosphoribosylaminoimidazole carboxylase 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2
    EC 6.3.4.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide synthase ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 385.4)
    and 11 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 112.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 38.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 37.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 37.1)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 35.9)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 29.9)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 23.4)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 20.4)
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 18.8)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 18.4)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 11.9)
  • TheSEED  :
    • Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)
  • PFAM:
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 160.9)
    and 21 more
    CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 48.3)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 27.1)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 27)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 24.5)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 23.5)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 21.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 19.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.6)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 16.5)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 16.4)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 15.8)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 15)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.3)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 13.7)
    NADP_Rossmann (CL0063) NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 13.2)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 12.9)
    ATP-grasp (CL0179) ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 12.5)
    DUF1297; Domain of unknown function (DUF1297) (PF06973; HMM-score: 12.3)
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 12.2)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.243947
    • TAT(Tat/SPI): 0.024705
    • LIPO(Sec/SPII): 0.016745
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDCPCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAIQLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQVLINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQILFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSGHYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWHLHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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