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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01092
  • pan locus tag?: SAUPAN003374000
  • symbol: pheS
  • pan gene symbol?: pheS
  • synonym:
  • product: phenylalanyl-tRNA synthetase subunit alpha

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01092
  • symbol: pheS
  • product: phenylalanyl-tRNA synthetase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1051883..1052941
  • length: 1059
  • essential: yes [1] DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCTGAACAACAAACAATGTCAGAGTTAAAACAACAAGCGCTTGTAGATATTAATGAA
    GCAAATGATGAACGTGCACTGCAAGAAGTTAAAGTGAAATACTTAGGTAAAAAAGGGTCA
    GTTAGCGGACTAATGAAATTGATGAAGGATTTGCCGAATGAAGATAAACCTGCGTTTGGT
    CAAAAAGTGAATGAATTGCGTCAAACAATTCAAAATGAATTAGATGAAAGACAACAGATG
    TTAGTTAAAGAAAAATTAAATAAGCAATTGGCTGAAGAAACAATTGATGTATCATTACCA
    GGTCGTCATATTGAAATCGGTTCAAAGCATCCATTAACACGTACAATAGAAGAAATTGAA
    GACTTATTCTTAGGTTTAGGTTATGAAATTGTGAATGGATATGAAGTTGAACAAGATCAT
    TATAACTTCGAAATGCTGAATTTACCTAAATCACACCCTGCACGTGATATGCAAGATAGT
    TTCTATATTACGGATGAAATTTTATTACGTACGCATACATCACCAGTGCAGGCACGTACG
    ATGGAATCACGTCATGGTCAAGGTCCAGTTAAAATTATTTGCCCTGGTAAAGTGTATCGT
    CGTGACTCTGATGATGCGACACATAGTCATCAATTTACACAAATCGAAGGATTAGTTGTT
    GATAAAAACGTTAAAATGAGTGATTTGAAAGGTACTTTAGAATTGTTAGCTAAGAAATTA
    TTTGGTGCTGATCGTGAAATTCGTTTACGTCCAAGTTACTTCCCATTCACTGAACCTTCT
    GTAGAAGTTGATGTGTCATGTTTTAAATGTAAAGGAAAAGGTTGTAATGTGTGTAAACAC
    ACAGGATGGATTGAAATTTTAGGTGCTGGAATGGTACATCCTAATGTATTAGAAATGGCT
    GGTTTTGATTCTTCAGAGTACTCTGGATTTGCATTTGGTATGGGACCAGACCGTATTGCA
    ATGTTGAAATATGGTATAGAAGATATTCGTCATTTCTATACTAATGATGTGAGATTTTTA
    GATCAATTTAAAGCGGTAGAAGATAGAGGTGACATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1059

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01092
  • symbol: PheS
  • description: phenylalanyl-tRNA synthetase subunit alpha
  • length: 352
  • theoretical pI: 5.47515
  • theoretical MW: 40106.4
  • GRAVY: -0.540057

Function[edit | edit source]

  • reaction:
    EC 6.1.1.20?  ExPASy
    Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 380.5)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase (TIGR00469; EC 6.1.1.20; HMM-score: 124.3)
    Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 48.9)
    Metabolism Amino acid biosynthesis Serine family O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 48.9)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00471; EC 6.1.1.20; HMM-score: 18.9)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 15.6)
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 13.3)
    Genetic information processing Transcription Degradation of RNA ribonuclease, Rne/Rng family (TIGR00757; EC 3.1.4.-; HMM-score: 6.9)
  • TheSEED  :
    • Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)
    Protein Metabolism Protein biosynthesis tRNA aminoacylation, Phe  Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 361.7)
    and 3 more
    tRNA_bind_arm (CL0298) Phe_tRNA-synt_N; Aminoacyl tRNA synthetase class II, N-terminal domain (PF02912; HMM-score: 85)
    no clan defined ORF11CD3; ORF11CD3 domain (PF10549; HMM-score: 12.5)
    tRNA_synt_II (CL0040) tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 9.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003802
    • TAT(Tat/SPI): 0.003535
    • LIPO(Sec/SPII): 0.000434
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGLMKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGRHIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDSFYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGLVVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCNVCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYTNDVRFLDQFKAVEDRGDM

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: T-box(Phe) (transcription antitermination) regulon
    T-box(Phe)(5' cis-acting region)important in Amino acid metabolism; compare RegPrecise for N315 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. 5.0 5.1 5.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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